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Title: Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound

Abstract

The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phoshpate and/or ribulose 5-phospate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.

Inventors:
; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1354809
Patent Number(s):
9637766
Application Number:
15/186,202
Assignee:
The Regents of the University of California
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 Jun 17
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Kirby, James, Fortman, Jeffrey L., Nishimoto, Minobu, and Keasling, Jay D. Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound. United States: N. p., 2017. Web.
Kirby, James, Fortman, Jeffrey L., Nishimoto, Minobu, & Keasling, Jay D. Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound. United States.
Kirby, James, Fortman, Jeffrey L., Nishimoto, Minobu, and Keasling, Jay D. Tue . "Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound". United States. https://www.osti.gov/servlets/purl/1354809.
@article{osti_1354809,
title = {Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound},
author = {Kirby, James and Fortman, Jeffrey L. and Nishimoto, Minobu and Keasling, Jay D.},
abstractNote = {The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phoshpate and/or ribulose 5-phospate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {5}
}

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Works referenced in this record:

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journal, June 2004


Computational Analysis of the Evolution of 1-Deoxy-D-xylulose-5-phosphate Reductoisomerase, an Important Enzyme in Plant Terpene Biosynthesis
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Mutations in Escherichia coli aceE and ribB Genes Allow Survival of Strains Defective in the First Step of the Isoprenoid Biosynthesis Pathway
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A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia coli strains defective in 1-deoxy- d -xylulose 5-phosphate synthase
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