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Title: Microscopy imaging device with advanced imaging properties

Systems, methods and devices are implemented for microscope imaging solutions. One embodiment of the present disclosure is directed toward an epifluorescence microscope. The microscope includes an image capture circuit including an array of optical sensor. An optical arrangement is configured to direct excitation light of less than about 1 mW to a target object in a field of view of that is at least 0.5 mm.sup.2 and to direct epi-fluorescence emission caused by the excitation light to the array of optical sensors. The optical arrangement and array of optical sensors are each sufficiently close to the target object to provide at least 2.5 .mu.m resolution for an image of the field of view.
Inventors:
; ; ; ; ;
Issue Date:
OSTI Identifier:
1353096
Assignee:
The Board of Trustees of the Leland Stanford Junior University LLNL
Patent Number(s):
9,629,554
Application Number:
14/857,156
Contract Number:
A52-07NA27344
Resource Relation:
Patent File Date: 2015 Sep 17
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 47 OTHER INSTRUMENTATION

Other works cited in this record:

Blind Wiring Mach.
patent, March 1869

Broad band optical reduction system using matched multiple refractive element materials
patent, May 1993

In vivo video camera system
patent, February 1997

Apparatus for monitoring substances in organisms
patent, August 1998

Miniature scanning confocal microscope
patent, July 2000

Miniature microscope
patent, August 2000

Semiconductor based excitation illuminator for fluorescence and phosphorescence microscopy
patent, November 2000

Portable tissue spectroscopy apparatus and method
patent, November 2001

Electronic systems and component devices for macroscopic and microscopic molecular biological reactions, analyses and diagnostics
patent, August 2004

Encoded molecular sieve particle-based sensors
patent, September 2004

Capsule endoscope
patent, February 2005

Imaging system with an integrated source and detector array
patent, January 2006

Miniature microscope objective lens
patent, April 2006

Miniaturized microscope array digital slide scanner
patent, February 2007

EPI-illumination system for an array microscope
patent, March 2007

Method and apparatus for high resolution coherent optical imaging
patent, July 2007

Fluorescence correlation spectroscopy instrument and method of using the same
patent, September 2007

Laser crystallization apparatus and laser crystallization method
patent, November 2007

Micro-optical analysis system and approach therefor
patent, December 2007

Capsule endoscope and a capsule endoscope system
patent, January 2008

Compact fluorescence endoscopy video system
patent, March 2008

Wireless in-vivo information acquiring system and body-insertable device
patent, September 2008

Optical delivery systems and methods of providing adjustable beam diameter, spot size and/or spot shape
patent, October 2009

Active CMOS biosensor chip for fluorescent-based detection
patent, June 2010

Ingestible device platform for the colon
patent, June 2011

Ingestible pill for diagnosing a gastrointestinal tract
patent, October 2011

Device and method for wide-field and high resolution imaging of tissue
patent, October 2011

Method and system of using intrinsic-based photosensing with high-speed line scanning for characterization of biological thick tissue including muscle
patent, November 2011

Method and an apparatus for localization of single dye molecules in the fluorescent microscopy
patent, July 2012

Imaging chamber with window and micro-needle platform magnetically biased toward each other
patent, October 2012

Imaging apparatus and methods
patent, October 2012

Live being optical analysis system and approach
patent, July 2014

Vaccination by transcutaneous targeting
patent, February 2015

Microscopy imaging device with advanced imaging properties
patent, November 2015

A genetic screen identifies genes essential for development of myelinated axons in zebrafish
journal, October 2006
  • Pogoda, Hans-Martin; Sternheim, Nitzan; Lyons, David A.
  • Developmental Biology, Vol. 298, Issue 1, p. 118-131
  • DOI: 10.1016/j.ydbio.2006.06.021

Imaging performance of a miniature integrated microendoscope
journal, September 2008
  • Rogers, Jeremy D.; Landau, Sara; Tkaczyk, Tomasz S.
  • Journal of Biomedical Optics, Vol. 13, Issue 5, Article No. 054020
  • DOI: 10.1117/1.2978060

Lensfree holographic imaging for on-chip cytometry and diagnostics
journal, January 2009
  • Seo, Sungkyu; Su, Ting-Wei; Tseng, Derek K.
  • Lab Chip, Vol. 9, Issue 6, p. 777-787
  • DOI: 10.1039/B813943A

A Role for the Cerebellum in Learning Movement Coordination
journal, July 1998

Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators
journal, November 2009
  • Tian, Lin; Hires, S Andrew; Mao, Tianyi
  • Nature Methods, Vol. 6, Issue 12, p. 875-881
  • DOI: 10.1038/nmeth.1398

Confocal fluorescence microscope with dual-axis architecture and biaxial postobjective scanning
journal, January 2004
  • Wang, Thomas D.; Contag, Christopher H.; Mandella, Michael J.
  • Journal of Biomedical Optics, Vol. 9, Issue 4, p. 735-742
  • DOI: 10.1117/1.1760760

Advances in Light Microscopy for Neuroscience
journal, June 2009

The application of Fresnel zone plate based projection in optofluidic microscopy
journal, September 2008
  • Wu, Jigang; Cui, Xiquan; Lee, Lap Man
  • Optics Express, Vol. 16, Issue 20, p. 15595-15602
  • DOI: 10.1364/OE.16.015595

Integrated semiconductor optical sensors for cellular and neural imaging
journal, April 2007
  • Levi, Ofer; Lee, Thomas T.; Lee, Meredith M.
  • Applied Optics, Vol. 46, Issue 10, p. 1881-1889
  • DOI: 10.1364/AO.46.001881

Miniaturized integration of a fluorescence microscope
journal, September 2011
  • Ghosh, Kunal K.; Burns, Laurie D.; Cocker, Eric D.
  • Nature Methods, Vol. 8, Issue 10, p. 871-878
  • DOI: 10.1038/nmeth.1694

A Miniature Head-Mounted Two-Photon Microscope
journal, September 2001


conference, May 2009
  • Park, Joon Hyuk; Pieribone, Vincent; Kim, Dongsoo
  • 2009 IEEE International Symposium on Circuits and Systems
  • DOI: 10.1109/ISCAS.2009.5118291

Listening to Brain Microcircuits for Interfacing With External World—Progress in Wireless Implantable Microelectronic Neuroengineering Devices
journal, March 2010
  • Nurmikko, Arto V.; Donoghue, John P.; Hochberg, Leigh R.
  • Proceedings of the IEEE, Vol. 98, Issue 3, p. 375-388
  • DOI: 10.1109/JPROC.2009.2038949

Imaging Large-Scale Neural Activity with Cellular Resolution in Awake, Mobile Mice
journal, October 2007

In vivo brain imaging using a portable 29 g two-photon microscope based on a microelectromechanical systems scanning mirror
journal, August 2009
  • Piyawattanametha, Wibool; Cocker, Eric D.; Burns, Laurie D.
  • Optics Letters, Vol. 34, Issue 15, p. 2309-2311
  • DOI: 10.1364/OL.34.002309

Visually evoked activity in cortical cells imaged in freely moving animals
journal, November 2009
  • Sawinski, J.; Wallace, D. J.; Greenberg, D. S.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 46, p. 19557-19562
  • DOI: 10.1073/pnas.0903680106

Spatiotemporal Dynamics of Cortical Sensorimotor Integration in Behaving Mice
journal, December 2007

Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo
journal, January 2008
  • Engelbrecht, Christoph J.; Johnston, Richard S.; Seibel, Eric J.
  • Optics Express, Vol. 16, Issue 8, 5556
  • DOI: 10.1364/OE.16.005556

Climbing fiber microzones in cerebellar vermis and their projection to different groups of cells in the lateral vestibular nucleus
journal, August 1978
  • Andersson, G.; Oscarsson, O.
  • Experimental Brain Research, Vol. 32, Issue 4, p. 565-579
  • DOI: 10.1007/BF00239553

Complex spikes in Purkinje cells in the lateral vermis (b zone) of the cat cerebellum during locomotion.
journal, April 1987

Anatomical and physiological foundations of cerebellar information processing
journal, March 2005
  • Apps, Richard; Garwicz, Martin
  • Nature Reviews Neuroscience, Vol. 6, Issue 4, p. 297-311
  • DOI: 10.1038/nrn1646

Time-lapse imaging of disease progression in deep brain areas using fluorescence microendoscopy
journal, January 2011
  • Barretto, Robert P. J.; Ko, Tony H.; Jung, Juergen C.
  • Nature Medicine, Vol. 17, Issue 2, p. 223-228
  • DOI: 10.1038/nm.2292

Mobile Phone Based Clinical Microscopy for Global Health Applications
journal, July 2009


journal, January 2002
  • Brinkmann, Marlies; Lütkemeyer, Dirk; Gudermann, Frank
  • Cytotechnology, Vol. 38, Issue 1/3, p. 119-127
  • DOI: 10.1023/A:1021118501866

Temporal Structure of Receptor Neuron Input to the Olfactory Bulb Imaged in Behaving Rats
journal, February 2009
  • Carey, Ryan M.; Verhagen, Justus V.; Wesson, Daniel W.
  • Journal of Neurophysiology, Vol. 101, Issue 2, p. 1073-1088
  • DOI: 10.1152/jn.90902.2008

Lensless high-resolution on-chip optofluidic microscopes for Caenorhabditis elegans and cell imaging
journal, July 2008
  • Cui, X.; Lee, L. M.; Heng, X.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 31, p. 10670-10675
  • DOI: 10.1073/pnas.0804612105

CMOS image sensors
journal, May 2005
  • El Gamal, A.; Eltoukhy, H.
  • IEEE Circuits and Devices Magazine, Vol. 21, Issue 3, p. 6-20
  • DOI: 10.1109/MCD.2005.1438751

Reliable Coding Emerges from Coactivation of Climbing Fibers in Microbands of Cerebellar Purkinje Neurons
journal, August 2009

In vivo brain imaging using a portable 39 gram two-photon fluorescence microendoscope
journal, September 2005
  • Flusberg, Benjamin A.; Jung, Juergen C.; Cocker, Eric D.
  • Optics Letters, Vol. 30, Issue 17, p. 2272-2274
  • DOI: 10.1364/OL.30.002272

The isochronic band hypothesis and climbing fibre regulation of motricity: an experimental study: Global patterns of climbing fibre activity
journal, July 2009

Optofluidic microscopy—a method for implementing a high resolution optical microscope on a chip
journal, January 2006
  • Heng, Xin; Erickson, David; Baugh, L. Ryan
  • Lab Chip, Vol. 6, Issue 10, p. 1274-1276
  • DOI: 10.1039/B604676B

Functional organization of climbing fibre projection to the cerebellar anterior lobe of the rat
journal, January 2000

In Vivo Mammalian Brain Imaging Using One- and Two-Photon Fluorescence Microendoscopy
journal, November 2004
  • Jung, Juergen C.; Mehta, Amit D.; Aksay, Emre
  • Journal of Neurophysiology, Vol. 92, Issue 5, p. 3121-3133
  • DOI: 10.1152/jn.00234.2004

Inhibitory Cerebello-Olivary Projections and Blocking Effect in Classical Conditioning
journal, January 1998

Somatotopic studies on the vermal cortex of the cerebellar anterior lobe of unanaesthetized cats
journal, April 1977
  • Leicht, R.; Schmidt, R.F.
  • Experimental Brain Research, Vol. 27, Issue 5, p. 479-490
  • DOI: 10.1007/BF00239037

A genetically encoded calcium indicator for chronic in vivo two-photon imaging
journal, August 2008
  • Mank, Marco; Santos, Alexandre Ferrão; Direnberger, Stephan
  • Nature Methods, Vol. 5, Issue 9, p. 805-811
  • DOI: 10.1038/nmeth.1243

Automated Analysis of Cellular Signals from Large-Scale Calcium Imaging Data
journal, September 2009

Dendritic encoding of sensory stimuli controlled by deep cortical interneurons
journal, January 2009
  • Murayama, Masanori; Pérez-Garci, Enrique; Nevian, Thomas
  • Nature, Vol. 457, Issue 7233, p. 1137-1141
  • DOI: 10.1038/nature07663

Motor Behavior Activates Bergmann Glial Networks
journal, May 2009

Identification and Clustering of Event Patterns From In Vivo Multiphoton Optical Recordings of Neuronal Ensembles
journal, July 2008
  • Ozden, Ilker; Lee, H. Megan; Sullivan, Megan R.
  • Journal of Neurophysiology, Vol. 100, Issue 1, p. 495-503
  • DOI: 10.1152/jn.01310.2007

High-throughput fluorescence microscopy for systems biology
journal, July 2006
  • Pepperkok, Rainer; Ellenberg, Jan
  • Nature Reviews Molecular Cell Biology, Vol. 7, Issue 9, p. 690-696
  • DOI: 10.1038/nrm1979

In Vivo Multiphoton Microscopy of Deep Brain Tissue
journal, April 2004
  • Levene, Michael J.; Dombeck, Daniel A.; Kasischke, Karl A.
  • Journal of Neurophysiology, Vol. 91, Issue 4, p. 1908-1912
  • DOI: 10.1152/jn.01007.2003

A focusing image probe for assessing neural activity in vivo
journal, September 1999
  • Rector, David M; Rogers, Robert F; George, John S
  • Journal of Neuroscience Methods, Vol. 91, Issue 1-2, p. 135-145
  • DOI: 10.1016/S0165-0270(99)00088-6

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