Methods for increasing production of 3-methyl-2-butenol using fusion proteins
Abstract
The present invention relates to methods and compositions for increasing production of 3-methyl-2-butenol in a de novo synthetic pathway in a genetically modified host cell using isopentenyl disphosphate (IPP) as a substrate.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1353075
- Patent Number(s):
- 9631210
- Application Number:
- 14/345,147
- Assignee:
- The Regents of the University of California
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Sep 13
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Chou, Howard, and Keasling, Jay D. Methods for increasing production of 3-methyl-2-butenol using fusion proteins. United States: N. p., 2017.
Web.
Chou, Howard, & Keasling, Jay D. Methods for increasing production of 3-methyl-2-butenol using fusion proteins. United States.
Chou, Howard, and Keasling, Jay D. Tue .
"Methods for increasing production of 3-methyl-2-butenol using fusion proteins". United States. https://www.osti.gov/servlets/purl/1353075.
@article{osti_1353075,
title = {Methods for increasing production of 3-methyl-2-butenol using fusion proteins},
author = {Chou, Howard and Keasling, Jay D.},
abstractNote = {The present invention relates to methods and compositions for increasing production of 3-methyl-2-butenol in a de novo synthetic pathway in a genetically modified host cell using isopentenyl disphosphate (IPP) as a substrate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {4}
}
Works referenced in this record:
Structural genomics of enzymes involved in sterol/isoprenoid biosynthesis
journal, November 2001
- Bonanno, J. B.; Edo, C.; Eswar, N.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 23, p. 12896-12901
A Soluble Form of Phosphatase in Saccharomyces cerevisiae Capable of Converting Farnesyl Diphosphate Into E-E Farnesol
journal, January 2006
- Song, Linsheng
- Applied Biochemistry and Biotechnology, Vol. 128, Issue 2, p. 149-158
Identification of Cys139 and Glu207 As Catalytically Important Groups in the Active Site of Isopentenyl Diphosphate:Dimethylallyl Diphosphate Isomerase
journal, April 1994
- Street, Ian P.; Coffman, Hazel R.; Poulter, C. Dale
- Biochemistry, Vol. 33, Issue 14, p. 4212-4217
Catalytic Mechanism of Escherichia coli Isopentenyl Diphosphate Isomerase Involves Cys-67, Glu-116, and Tyr-104 as Suggested by Crystal Structures of Complexes with Transition State Analogues and Irreversible Inhibitors
journal, January 2003
- Wouters, J.; Oudjama, Y.; Barkley, Sam J.
- Journal of Biological Chemistry, Vol. 278, Issue 14, p. 11903-11908
Crystal Structures of Human IPP Isomerase: New Insights into the Catalytic Mechanism
journal, March 2007
- Zhang, Cheng; Liu, Lin; Xu, Hang
- Journal of Molecular Biology, Vol. 366, Issue 5, p. 1437-1446
The Crystal Structure of Human Isopentenyl Diphosphate Isomerase at 1.7 Å Resolution Reveals its Catalytic Mechanism in Isoprenoid Biosynthesis
journal, March 2007
- Zheng, Wei; Sun, Fei; Bartlam, Mark
- Journal of Molecular Biology, Vol. 366, Issue 5, p. 1447-1458
Stereochemical Studies on the Making and Unmaking of Isopentenyl Diphosphate in Different Biological Systems
journal, September 2004
- Laupitz, Ralf; Gräwert, Tobias; Rieder, Christoph
- Chemistry & Biodiversity, Vol. 1, Issue 9, p. 1367-1376