skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Formation of stable submicron peptide or protein particles by thin film freezing

Abstract

The present invention includes compositions and methods for preparing micron-sized or submicron-sized particles by dissolving a water soluble effective ingredient in one or more solvents; spraying or dripping droplets solvent such that the effective ingredient is exposed to a vapor-liquid interface of less than 50, 100, 150, 200, 250, 200, 400 or 500 cm.sup.-1 area/volume to, e.g., increase protein stability; and contacting the droplet with a freezing surface that has a temperature differential of at least 30.degree. C. between the droplet and the surface, wherein the surface freezes the droplet into a thin film with a thickness of less than 500 micrometers and a surface area to volume between 25 to 500 cm.sup.-1.

Inventors:
; ;
Issue Date:
Research Org.:
Board of Regents, The University of Texas System, Austin, TX (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1351888
Patent Number(s):
9622974
Application Number:
14/603,211
Assignee:
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS
Patent Classifications (CPCs):
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
DOE Contract Number:  
FG02-04ER15549
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Jan 22
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 36 MATERIALS SCIENCE

Citation Formats

Johnston, Keith P., Engstrom, Joshua, and Williams, III, Robert O. Formation of stable submicron peptide or protein particles by thin film freezing. United States: N. p., 2017. Web.
Johnston, Keith P., Engstrom, Joshua, & Williams, III, Robert O. Formation of stable submicron peptide or protein particles by thin film freezing. United States.
Johnston, Keith P., Engstrom, Joshua, and Williams, III, Robert O. Tue . "Formation of stable submicron peptide or protein particles by thin film freezing". United States. https://www.osti.gov/servlets/purl/1351888.
@article{osti_1351888,
title = {Formation of stable submicron peptide or protein particles by thin film freezing},
author = {Johnston, Keith P. and Engstrom, Joshua and Williams, III, Robert O.},
abstractNote = {The present invention includes compositions and methods for preparing micron-sized or submicron-sized particles by dissolving a water soluble effective ingredient in one or more solvents; spraying or dripping droplets solvent such that the effective ingredient is exposed to a vapor-liquid interface of less than 50, 100, 150, 200, 250, 200, 400 or 500 cm.sup.-1 area/volume to, e.g., increase protein stability; and contacting the droplet with a freezing surface that has a temperature differential of at least 30.degree. C. between the droplet and the surface, wherein the surface freezes the droplet into a thin film with a thickness of less than 500 micrometers and a surface area to volume between 25 to 500 cm.sup.-1.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {4}
}

Patent:

Save / Share:

Works referenced in this record:

Commercial challenges of protein drug delivery
journal, January 2005


Advances in ultrarapid freezing for the preservation of cellular ultrastructure
journal, January 1986


Role of calcium phosphate nanoclusters in the control of calcification
journal, April 2009


Concentrated Dispersions of Equilibrium Protein Nanoclusters That Reversibly Dissociate into Active Monomers
journal, January 2012


Reversible Self-Association of a Concentrated Monoclonal Antibody Solution Mediated by Fab–Fab Interaction That Impacts Solution Viscosity
journal, October 2008


Protein Inhalation Powders: Spray Drying vs Spray Freeze Drying
journal, January 1999


Novel ultra-rapid freezing particle engineering process for enhancement of dissolution rates of poorly water-soluble drugs
journal, January 2007


Solution-Based Particle Formation of Pharmaceutical Powders by Supercritical or Compressed Fluid Co2 and Cryogenic Spray-Freezing Technologies
journal, January 2001


Spray freezing into liquid versus spray-freeze drying: Influence of atomization on protein aggregation and biological activity
journal, January 2006


Coarse-Grained Strategy for Modeling Protein Stability in Concentrated Solutions
journal, October 2005


Unusual properties of aqueous solutions of l-proline: A molecular dynamics study
journal, November 2005


Anomalously Large Equilibrium Clusters of Colloids
journal, November 2001


Inhibition of protein aggregation in vitro and in vivo by a natural osmoprotectant
journal, August 2006


Enhancement of thermal stability and inhibition of protein aggregation by osmolytic effect of hydroxyproline
journal, January 2007


Theoretical analysis of excipient concentrations during the final ultrafiltration/diafiltration step of therapeutic antibody
journal, July 2009


Thermophysical Properties of Trehalose and Its Concentrated Aqueous Solutions
journal, January 1997


Phase Behavior of Small Attractive Colloidal Particles
journal, January 1996


Coarse-Grained Strategy for Modeling Protein Stability in Concentrated Solutions. II: Phase Behavior
journal, March 2006


Challenges in the development of high protein concentration formulations
journal, June 2004


Protein−solute interactions affect the outcome of ultrafiltration/diafiltration operations
journal, September 2004


Is Random Close Packing of Spheres Well Defined?
journal, March 2000


Molecular Mechanisms of Cryoprotection in Aqueous Proline:  Light Scattering and Molecular Dynamics Simulations
journal, April 2008


Enhancement of Protein Crystal Nucleation by Critical Density Fluctuations
journal, September 1997


The thermodynamic mechanism of protein stabilization by trehalose
journal, February 1997


Structure and thermodynamics of colloidal protein cluster formation: Comparison of square-well and simple dipolar models
journal, September 2009


Macromolecular Crowding and Confinement: Biochemical, Biophysical, and Potential Physiological Consequences
journal, June 2008