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Title: Detection of protease and protease activity using a single nanocrescent SERS probe

This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.
Inventors:
; ; ;
Issue Date:
OSTI Identifier:
1351818
Assignee:
The Regents of the University of California (Oakland, CA) LBNL
Patent Number(s):
9,145,575
Application Number:
13/752,069
Contract Number:
AC02-05CH11231
Resource Relation:
Patent File Date: 2013 Jan 28
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; 60 APPLIED LIFE SCIENCES

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