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Title: Detection of protease and protease activity using a single nanocrescent SERS probe

Abstract

This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.

Inventors:
; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1351818
Patent Number(s):
9145575
Application Number:
13/752,069
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Jan 28
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; 60 APPLIED LIFE SCIENCES

Citation Formats

Liu, Gang L., Ellman, Jonathan A., Lee, Luke P., and Chen, Fanqing Frank. Detection of protease and protease activity using a single nanocrescent SERS probe. United States: N. p., 2015. Web.
Liu, Gang L., Ellman, Jonathan A., Lee, Luke P., & Chen, Fanqing Frank. Detection of protease and protease activity using a single nanocrescent SERS probe. United States.
Liu, Gang L., Ellman, Jonathan A., Lee, Luke P., and Chen, Fanqing Frank. Tue . "Detection of protease and protease activity using a single nanocrescent SERS probe". United States. https://www.osti.gov/servlets/purl/1351818.
@article{osti_1351818,
title = {Detection of protease and protease activity using a single nanocrescent SERS probe},
author = {Liu, Gang L. and Ellman, Jonathan A. and Lee, Luke P. and Chen, Fanqing Frank},
abstractNote = {This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {9}
}

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