Global to push GA events into
skip to main content

Title: Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate

One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce lactate or acetate as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of lactate or acetate. Another aspect of the invention relates to a process for converting lignocellulosic biomass to lactate or acetate, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.
Inventors:
; ; ; ; ; ; ; ; ; ;
Issue Date:
OSTI Identifier:
1348376
Assignee:
Lallemand Hungary Liquidity Management LLC (Budapest, HU) GFO
Patent Number(s):
9,605,269
Application Number:
13/696,207
Contract Number:
FC36-08GO18103; FC36-07GO17057
Resource Relation:
Patent File Date: 2011 May 05
Research Org:
Lallemand Hungary Liquidity Management LLC, Budapest, HU (Hungary)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Metabolically engineered microbial cell with an altered metabolite production
patent, March 2006

L-arabinose fermenting yeast
patent, December 2010

Primary alcohol producing organisms
patent, July 2011

Xylose-fermenting yeast engineered to increase flux of the pentose phosphate pathway
patent, October 2011

Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids
patent, December 2011

Recombinant thermotolerant yeast with a substitute heat shock protein 104 promoter
patent, March 2014

Genetically transformed microorganisms with simultaneous enhancement of reduction potential and reductive enzyme activities for biomass fermentation
patent, June 2014

Alcohol dehydrogenases (ADH) useful for fermentive production of lower alkyl alcohols
patent, July 2014

Fermentative glycerol-free ethanol production
patent, August 2014

Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors
patent, February 2015

Elimination of Glycerol Production in Anaerobic Cultures of a Saccharomyces cerevisiae Strain Engineered To Use Acetic Acid as an Electron Acceptor
journal, November 2009
  • Medina, Victor; Almering, Marinka; van Maris, Antonius
  • Applied and Environmental Microbiology, Vol. 76, Issue 1, p. 190-195
  • DOI: 10.1128/AEM.01772-09

Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae
journal, January 2007
  • Almeida, Joao; Modig, Tobias; Petersson, Anneli
  • Journal of Chemical Technology & Biotechnology, Vol. 82, Issue 4, p. 340-349
  • DOI: 10.1002/jctb.1676

An improvement in Pichia stipitis fermentation of acid-hydrolysed hemicellulose achieved by overliming (calcium hydroxide treatment) and strain adaptation
journal, May 1996
  • Amartey, S.; Jeffries, T.
  • World Journal of Microbiology & Biotechnology, Vol. 12, Issue 3, p. 281-283
  • DOI: 10.1007/BF00360928

Comparative genome analysis of a Saccharomyces cerevisiae wine strain
journal, November 2008

Cloning of L-lactate dehydrogenase and elimination of lactic acid production via gene knockout in Thermoanaerobacterium saccharolyticum JW/SL-YS485
journal, March 2004

Minimization of glycerol synthesis in industrial ethanol yeast without influencing its fermentation performance
journal, January 2011

Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production
journal, November 2008
  • Guo, Zhong-peng; Zhang, Liang; Ding, Zhong-yang
  • Applied Microbiology and Biotechnology, Vol. 82, Issue 2, p. 287-292
  • DOI: 10.1007/s00253-008-1777-7

Enhanced ethanol production by fermentation of rice straw hydrolysate without detoxification using a newly adapted strain of Pichia stipitis
journal, September 2009

Reduced Oxidative Pentose Phosphate Pathway Flux in Recombinant Xylose-Utilizing Saccharomyces cerevisiae Strains Improves the Ethanol Yield from Xylose
journal, April 2002
  • Jeppsson, Marie; Johansson, Bjorn; Hahn-Hagerdal, Barbel
  • Applied and Environmental Microbiology, Vol. 68, Issue 4, p. 1604-1609
  • DOI: 10.1128/AEM.68.4.1604-1609.2002

The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiaestrains
journal, October 2003
  • Jeppsson, Marie; Johansson, Bjorn; Jensen, Peter Ruhdal
  • Yeast, Vol. 20, Issue 15, p. 1263-1272
  • DOI: 10.1002/yea.1043

The cytostat: A new way to study cell physiology in a precisely defined environment
journal, November 2006

Comparison of the xylose reductase-xylitol dehydrogenase and the xylose isomerase pathways for xylose fermentation by recombinant Saccharomyces cerevisiae
journal, January 2007
  • Karhumaa, Kaisa; Sanchez, Rosa; Hahn-Hagerdal, Bärbel
  • Microbial Cell Factories, Vol. 6, Issue 1, 5 p.
  • DOI: 10.1186/1475-2859-6-5

Minimal metabolic engineering of for efficient anaerobic xylose fermentation a proof of principle
journal, March 2004

Metabolic engineering of a xylose-isomerase-expressing strain for rapid anaerobic xylose fermentation
journal, February 2005

Evolutionary engineering of mixed-sugar utilization by a xylose-fermenting strain
journal, July 2005

Purification, characterization and functional analysis of an endo-arabinanase (AbnA) from Bacillus subtilis
journal, December 2004

Detoxification of woody hydrolyzates with activated carbon for bioconversion to ethanol by the thermophilic anaerobic bacterium Thermoanaerobacterium saccharolyticum
journal, January 2011

Effect of Increased Yeast Alcohol Acetyltransferase Activity on Flavor Profiles of Wine and Distillates
journal, February 2000
  • Lilly, M.; Lambrechts, M.; Pretorius, I.
  • Applied and Environmental Microbiology, Vol. 66, Issue 2, p. 744-753
  • DOI: 10.1128/AEM.66.2.744-753.2000

Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
  • Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
  • Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
  • DOI: 10.1128/MMBR.66.3.506-577.2002

High-Value Renewable Energy from Prairie Grasses
journal, May 2002
  • McLaughlin, S.; de la Torre Ugarte, D.; Garten, C.
  • Environmental Science & Technology, Vol. 36, Issue 10, p. 2122-2129
  • DOI: 10.1021/es010963d

Control of the Arabinose Regulon in Bacillus subtilis by AraR In Vivo: Crucial Roles of Operators, Cooperativity, and DNA Looping
journal, July 2001
  • Mota, Luis Jaime; Sarmento, Leonor Morais; de Sa-Nogueira, Isabel
  • Journal of Bacteriology, Vol. 183, Issue 14, p. 4190-4201
  • DOI: 10.1128/JB.183.14.4190-4201.2001

Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis
journal, August 1999

Codon usage tabulated from international DNA sequence databases: status for the year 2000
journal, January 2000
  • Nakamura, Yasukazu; Gojobori, Takashi; Ikemura, Toshimichi
  • Nucleic Acids Research, Vol. 28, Issue 1, p. 292-292
  • DOI: 10.1093/nar/28.1.292

The Yeast Glycerol 3-Phosphatases Gpp1p and Gpp2p Are Required for Glycerol Biosynthesis and Differentially Involved in the Cellular Responses to Osmotic, Anaerobic, and Oxidative Stress
journal, October 2000
  • Pahlman, Anna-Karin; Granath, Katarina; Ansell, Ricky
  • Journal of Biological Chemistry, Vol. 276, Issue 5, p. 3555-3563
  • DOI: 10.1074/jbc.M007164200

Quantitative evaluation of yeast's requirement for glycerol formation in very high ethanol performance fed-batch process
journal, January 2010
  • Pagliardini, Julien; Hubmann, Georg; Bideaux, Carine
  • Microbial Cell Factories, Vol. 9, Issue 1
  • DOI: 10.1186/1475-2859-9-36

The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression
journal, March 1997

Regulation of the L-arabinose operon of Escherichia coli
journal, December 2000

Selective removal of acetic acid from hardwood-spent sulfite liquor using a mutant yeast
journal, August 1996

Saccharomyces cerevisiae-Based Molecular Tool Kit for Manipulation of Genes from Gram-Negative Bacteria
journal, July 2006
  • Shanks, Robert; Caiazza, Nicky; Hinsa, Shannon
  • Applied and Environmental Microbiology, Vol. 72, Issue 7, p. 5027-5036
  • DOI: 10.1128/AEM.00682-06

Identification of the [FeFe]-Hydrogenase Responsible for Hydrogen Generation in Thermoanaerobacterium saccharolyticum and Demonstration of Increased Ethanol Yield via Hydrogenase Knockout
journal, July 2009
  • Shaw, A. Joe; Hogsett, David; Lynd, Lee
  • Journal of Bacteriology, Vol. 191, Issue 20, p. 6457-6464
  • DOI: 10.1128/JB.00497-09

Natural Competence in Thermoanaerobacter and Thermoanaerobacterium Species
journal, May 2010
  • Shaw, A.; Hogsett, D.; Lynd, L.
  • Applied and Environmental Microbiology, Vol. 76, Issue 14, p. 4713-4719
  • DOI: 10.1128/AEM.00402-10

Marker Removal System for Thermoanaerobacterium saccharolyticum and Development of a Markerless Ethanologen
journal, February 2011
  • Shaw, A. Joe; Covalla, Sean; Hogsett, David
  • Applied and Environmental Microbiology, Vol. 77, Issue 7, p. 2534-2536
  • DOI: 10.1128/AEM.01731-10

Improved ethanol production by glycerol-3-phosphate dehydrogenase mutants of Saccharomyces cerevisiae
journal, October 1998
  • Valadi, H.; Larsson, C.; Gustafsson, L.
  • Applied Microbiology and Biotechnology, Vol. 50, Issue 4, p. 434-439
  • DOI: 10.1007/s002530051317

The Two Acetyl-coenzyme A Synthetases of Saccharomyces cerevisiae Differ with Respect to Kinetic Properties and Transcriptional Regulation
journal, November 1996
  • van den Berg, Marco; de Jong-Gubbels, Patricia; Kortland, Christine
  • Journal of Biological Chemistry, Vol. 271, Issue 46, p. 28953-28959
  • DOI: 10.1074/jbc.271.46.28953

Cloning, Expression, and Characterization of Bacterial L-Arabinose 1-Dehydrogenase Involved in an Alternative Pathway of L-Arabinose Metabolism
journal, December 2005
  • Watanabe, Seiya; Kodak, Tsutomu; Makino, Keisuke
  • Journal of Biological Chemistry, Vol. 281, Issue 5, p. 2612-2623
  • DOI: 10.1074/jbc.M506477200

The Acetate Switch
journal, March 2005

Similar records in DOepatents and OSTI.GOV collections: