Global to push GA events into
skip to main content

Title: Antibody profiling sensitivity through increased reporter antibody layering

A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.
Issue Date:
OSTI Identifier:
Battelle Energy Alliance, LLC INL
Patent Number(s):
Application Number:
Contract Number:
AC07-99ID13727; AC07-94ID13223; AC07-05ID14517
Resource Relation:
Patent File Date: 2013 Feb 20
Research Org:
Idaho National Lab. (INL), Idaho Falls, ID (United States)
Sponsoring Org:
Country of Publication:
United States

Other works cited in this record:

Marking Instrument
patent, August 1896

Assay employing a labeled Fab-fragment ligand complex
patent, November 1980

Phycobiliprotein fluorescent conjugates
patent, September 1985

Individual-specific antibody identification methods
patent, November 1989

Analytical test devices for competition assay for drugs of non-protein antigens using immunochromatographic techniques
patent, June 1990

Methods of identification employing antibody profiles
patent, December 1993

Modified avidin-biotin technique
patent, January 1994

Array of oligonucleotides on a solid substrate
patent, August 1995

Method of detecting and correcting a direction of image data and document image filing system employing the same
patent, November 1995

Anti-idiotypic monoclonal antibody that induces an immune response against the ganglioside GD3 and hybridoma producing said antibody
patent, June 1996

Method for detecting an analyte using an electrochemical luminescent transition metal label
patent, July 1996

Active programmable electronic devices for molecular biological analysis and diagnostics
patent, February 1997

Arrays of materials attached to a substrate
patent, April 1998

Patterning antibodies on a surface
patent, January 1999

Method of obtaining small conformationally rigid conopeptides
patent, March 1999

Monoclonal antibodies against human pancreatic islet cells
patent, March 1999

Miniaturized cell array methods and apparatus for cell-based screening
patent, August 2000

Method and apparatus for automated image analysis of biological specimens
patent, April 2003

Method and apparatus for acquiring offset checkshot survey data using tube-wave conversion
patent, July 2003

Method and system for extracting data from surface array deposited features
patent, July 2003

Aminediols for the treatment of Alzheimer's disease
patent, June 2005

System, method, and computer software product for grid alignment of multiple scanned images
patent, November 2005

Method, system, and computer code for finding spots defined in biological microarrays
patent, December 2005

Rapid classification of biological components
patent, January 2006

Systems and methods for automated analysis of cells and tissues
patent, May 2007

Rapid classification of biological components
patent, March 2010

Rapid classification of biological components
patent, March 2010

Antibody profiling sensitivity through increased reporter antibody layering
patent, April 2010

Micro-array analysis system and method thereof
patent, September 2011

Antibody profiling sensitivity through increased reporter antibody layering
patent, February 2013

Rapid classification of biological components
patent, October 2013

Pattern Recognition in Pulmonary Tuberculosis Defined by High Content Peptide Microarray Chip Analysis Representing 61 Proteins from M. tuberculosis
journal, December 2008
  • Gaseitsiwe, Simani; Valentini, Davide; Mahdavifar, Shahnaz
  • PLoS ONE, Vol. 3, Issue 12, Article No. e3840
  • DOI: 10.1371/journal.pone.0003840

Cloning of open reading frames and promoters from the Saccharomyces cerevisiae genome: construction of genomic libraries of random small fragments
journal, January 1986

Drugs of Abuse in Saliva: A Review
journal, January 1992
  • Schramm, W.; Smith, R. H.; Craig, P. A.
  • Journal of Analytical Toxicology, Vol. 16, Issue 1, p. 1-9
  • DOI: 10.1093/jat/16.1.1

Multilayer peroxidase-labeled antibody method: Comparison with labeled streptavidin-biotin method, avidin-biotin-peroxidase complex method, and peroxidase-antiperoxidase method
journal, January 1995
  • Shin, Masashi; Izumi, Shin-Ichi; Nakane, Paul K.
  • Journal of Clinical Laboratory Analysis, Vol. 9, Issue 6, p. 424-430
  • DOI: 10.1002/jcla.1860090615

Rapid Detection and Enumeration of Legionella pneumophila in Hot Water Systems by Solid-Phase Cytometry
journal, March 2004

Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions
journal, January 2001

Detection of analyte binding to microarrays using gold nanoparticle labels and a desktop scanner
journal, September 2003
  • Han, Anpan; Dufva, Martin; Belleville, Erik
  • Lab on a Chip, Vol. 3, Issue 4, p. 329-332
  • DOI: 10.1039/B310814G

Fiber-Optic Immunosensor for the Detection of Fumonisin B1
journal, April 1996
  • Thompson, Vicki S.; Maragos, Chris M.
  • Journal of Agricultural and Food Chemistry, Vol. 44, Issue 4, p. 1041-1046
  • DOI: 10.1021/jf950661t

Antibody profiling as an identification tool for forensic samples
conference, February 1999
  • Thompson, Vicki S.; Barrett, Karen B.; Davis, Tilton
  • Enabling Technologies for Law Enforcement and Security, vp.
  • DOI: 10.1117/12.334545

Difference gel electrophoresis. A single gel method for detecting changes in protein extracts
journal, January 1997
  • Ünlü, Mustafa; Morgan, Mary E.; Minden, Jonathan S.
  • Electrophoresis, Vol. 18, Issue 11, p. 2071-2077
  • DOI: 10.1002/elps.1150181133

Preliminary Investigations into Tris(2,2'-bipyridyl) Ruthenium (III) as a Chemiluminescent Reagent for the Detection of 3,6-Diacetylmorphine (Heroin) on Surfaces
journal, September 2007

Micromosaic Immunoassays
journal, November 2000
  • Bernard, André; Michel, Bruno; Delamarche, Emmanuel
  • Analytical Chemistry, Vol. 73, Issue 1, p. 8-12
  • DOI: 10.1021/ac0008845

Homogeneous enzyme immunoassay
journal, June 1992

Sensitivity of Two Enzyme-linked Immunosorbent Assay Tests in Relation to Western Blot in Detecting Human T-Cell Lymphotropic Virus Types I and II Infection among HIV-1 Infected Patients from São Paulo, Brazil
journal, March 1998
  • Caterino-de-Araujo, Adele; de los Santos-Fortuna, Elizabeth; Meleiro, Mônica Cristina Zandoná
  • Diagnostic Microbiology and Infectious Disease, Vol. 30, Issue 3, p. 173-182
  • DOI: 10.1016/S0732-8893(97)00236-8

Saliva Testing for Drugs of Abuse
journal, September 1993

Peptides on phage: a vast library of peptides for identifying ligands
journal, August 1990
  • Cwirla, S. E.; Peters, E. A.; Barrett, R. W.
  • Proceedings of the National Academy of Sciences, Vol. 87, Issue 16, p. 6378-6382
  • DOI: 10.1073/pnas.87.16.6378

Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale
journal, October 1997

Automatic multiparameter fluorescence imaging for determining lymphocyte phenotype and activation status in melanoma tissue sections
journal, September 1996

Advances in ELISA and other solid-phase immunoassays
journal, March 1986

Testing for drugs of abuse in saliva and sweat
journal, August 1998
  • Kidwell, David A.; Holland, Janel C.; Athanaselis, Sotiris
  • Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 713, Issue 1, p. 111-135
  • DOI: 10.1016/S0378-4347(97)00572-0

Electrogenerated Chemiluminescence: An Oxidative-Reduction Type ECL Reaction Sequence Using Tripropyl Amine
journal, January 1990
  • Leland, Jonathan K.; Powell, Michael J.
  • Journal of The Electrochemical Society, Vol. 137, Issue 10, p. 3127-3131
  • DOI: 10.1149/1.2086171

Similar records in DOepatents and OSTI.GOV collections: