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Title: Use of Galerina marginata genes and proteins for peptide production

The present invention relates to compositions and methods comprising genes and peptides associated with cyclic peptides and cyclic peptide production in mushrooms. In particular, the present invention relates to using genes and proteins from Galerina species encoding peptides specifically relating to amatoxins in addition to proteins involved with processing cyclic peptide toxins. In a preferred embodiment, the present invention also relates to methods for making small peptides and small cyclic peptides including peptides similar to amanitin. Further, the present inventions relate to providing kits for making small peptides.
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Patent File Date: 2015 Nov 05
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United States

Works referenced in this record:

An Extended Physical Map of the TOX2 Locus of Cochliobolus carbonum Required for Biosynthesis of HC-Toxin
journal, February 2002
  • Ahn, Joong-Hoon; Cheng, Yi-Qiang; Walton, Jonathan D.
  • Fungal Genetics and Biology, Vol. 35, Issue 1, p. 31-38
  • DOI: 10.1006/fgbi.2001.1305

Highly Efficient Production of Laccase by the Basidiomycete Pycnoporus cinnabarinus
journal, November 2004
  • Alves, Alexandra M. C. R.; Record, Eric; Lomascolo, Anne
  • Applied and Environmental Microbiology, Vol. 70, Issue 11, p. 6379-6384
  • DOI: 10.1128/AEM.70.11.6379-6384.2004

Proteins of the ADF/Cofilin Family: Essential Regulators of Actin Dynamics
journal, November 1999

Chemical Variation in Amanita
journal, July 1981
  • Beutler, John A.; Der Marderosian, Ara H.
  • Journal of Natural Products, Vol. 44, Issue 4, p. 422-431
  • DOI: 10.1021/np50016a005

Suggested functions for prolyl oligopeptidase: A puzzling paradox
journal, February 2007
  • Brandt, Inger; Scharpe, Simon; Lambeir, Anne-Marie
  • Clinica Chimica Acta, Vol. 377, Issue 1-2, p. 50-61
  • DOI: 10.1016/j.cca.2006.09.001

Efficient oxidative folding of conotoxins and the radiation of venomous cone snails
journal, October 2003
  • Bulaj, Grzegorz; Buczek, Olga; Goodsell, Ian
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue Supplement 2, p. 14562-14568
  • DOI: 10.1073/pnas.2335845100

Structural basis of transcription: α-Amanitin-RNA polymerase II cocrystal at 2.8 Å resolution
journal, January 2002
  • Bushnell, David A.; Cramer, Patrick; Kornberg, Roger D.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 3, p. 1218-1222
  • DOI: 10.1073/pnas.251664698

Diagnostic Accuracy of Urinary Amanitin in Suspected Mushroom Poisoning: A Pilot Study
journal, January 2004
  • Butera, Raffaella; Locatelli, Carlo; Coccini, Teresa
  • Journal of Toxicology: Clinical Toxicology, Vol. 42, Issue 6, p. 901-912
  • DOI: 10.1081/clt-200035472

Seamless Proteins Tie Up Their Loose Ends
journal, March 2006

The Chemistry and Biology of Cyclotides
journal, July 2007

WebLogo: A Sequence Logo Generator
journal, May 2004
  • Crooks, Gavin E.; Hon, Gary; Chandonia, John-Marc
  • Genome Research, Vol. 14, Issue 6, p. 1188-1190
  • DOI: 10.1101/gr.849004

Proline specific peptidases
journal, December 1997
  • Cunningham, Damian F.; O'Connor, Brendan
  • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1343, Issue 2, p. 160-186
  • DOI: 10.1016/S0167-4838(97)00134-9

Two Enzymes Catalyze the Maturation of a Lasso Peptide in Escherichia coli
journal, July 2007
  • Duquesne, Sophie; Destoumieux-Garzon, Delphine; Zirah, Severine
  • Chemistry & Biology, Vol. 14, Issue 7, p. 793-803
  • DOI: 10.1016/j.chembiol.2007.06.004

Treatment of Amatoxin Poisoning: 20-Year Retrospective Analysis
journal, January 2002
  • Enjalbert, Francoise; Rapior, Sylvie; Nouguier-Soule, Janine
  • Journal of Toxicology: Clinical Toxicology, Vol. 40, Issue 6, p. 715-757
  • DOI: 10.1081/CLT-120014646

Molecular diversification in spider venoms: A web of combinatorial peptide libraries
journal, November 2006

Biosynthesis of Nonribosomal Peptides
journal, October 2004

Deficient activity of mammalian prolyl oligopeptidase on the immunoactive peptide digestion in coeliac disease
journal, January 2007
  • Garcia-Horsman, J. Arturo; Venäläinen, Jarkko I.; Lohi, Olli
  • Scandinavian Journal of Gastroenterology, Vol. 42, Issue 5, p. 562-571
  • DOI: 10.1080/00365520601019819

The sirodesmin biosynthetic gene cluster of the plant pathogenic fungus Leptosphaeria maculans
journal, July 2004

Prolyl endopeptidases
journal, December 2006

Gene expression shifts during perithecium development in Gibberella zeae (anamorph Fusarium graminearum), with particular emphasis on ion transport proteins
journal, November 2007
  • Hallen, Heather E.; Huebner, Marianne; Shiu, Shin-Han
  • Fungal Genetics and Biology, Vol. 44, Issue 11, p. 1146-1156
  • DOI: 10.1016/j.fgb.2007.04.007

Gene family encoding the major toxins of lethal Amanita mushrooms
journal, November 2007
  • Hallen, Heather E.; Luo, Hong; Scott-Craig, John S.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 48, p. 19097-19101
  • DOI: 10.1073/pnas.0707340104

Taxonomy and toxicity of Conocybe lactea and related species
journal, August 2003
  • Hallen, Heather E.; Watling, Roy; Adams, Gerard C.
  • Mycological Research, Vol. 107, Issue 8, p. 969-979
  • DOI: 10.1017/S0953756203008190

Fungal secondary metabolism — from biochemistry to genomics
journal, December 2005
  • Keller, Nancy P.; Turner, Geoffrey; Bennett, Joan W.
  • Nature Reviews Microbiology, Vol. 3, Issue 12, p. 937-947
  • DOI: 10.1038/nrmicro1286

Size and complexity of the nuclear genome of the ectomycorrhizal fungus Paxillus involutus
journal, August 2002
  • Le Quere, Antoine; Johansson, Tomas; Tunlid, Anders
  • Fungal Genetics and Biology, Vol. 36, Issue 3, p. 234-241
  • DOI: 10.1016/S1087-1845(02)00024-5

Molecular Characterization and Inhibition of Amanitin Uptake into Human Hepatocytes
journal, February 2006
  • Letschert, Katrin; Faulstich, Heinz; Keller, Daniela
  • Toxicological Sciences, Vol. 91, Issue 1, p. 140-149
  • DOI: 10.1093/toxsci/kfj141

Specific Inhibition of Nuclear RNA Polymerase II by α-Amanitin
journal, October 1970

Introns are necessary for mRNA accumulation in Schizophyllum commune
journal, May 1999

Regulation of inducible and tissue-specific gene expression
journal, June 1987
  • Maniatis, T.; Goodbourn, S.; Fischer, J. A.
  • Science, Vol. 236, Issue 4806, p. 1237-1245
  • DOI: 10.1126/science.3296191

Genome sequencing in microfabricated high-density picolitre reactors
journal, July 2005
  • Margulies, Marcel; Egholm, Michael; Altman, William E.
  • Nature, Vol. 437, Issue 7057, p. 376-380
  • DOI: 10.1038/nature03959

Tryptathionine bridges in peptide synthesis
journal, January 2007
  • May, Jonathan P.; Perrin, David M.
  • Biopolymers, Vol. 88, Issue 5, p. 714-724
  • DOI: 10.1002/bip.20807

Effective production of amanitins by two-step cultivation of the basidiomycete, Galerina fasciculata GF-060
journal, January 2000

Identification of peptide synthetase-encoding genes from filamentous fungi producing host-selective phytotoxins or analogs
journal, January 1995
  • Nikolskaya, Anastasia N.; Panaccione, Daniel G.; Walton, Jonathan D.
  • Gene, Vol. 165, Issue 2, p. 207-211
  • DOI: 10.1016/0378-1119(95)00555-K

Quorum Sensing in Staphylococci
journal, December 2008

Conus Peptides: Biodiversity-based Discovery and Exogenomics
journal, August 2006
  • Olivera, Baldomero M.
  • Journal of Biological Chemistry, Vol. 281, Issue 42, p. 31173-31177
  • DOI: 10.1074/jbc.R600020200

Multiple families of peptide synthetase genes from ergopeptine-producing fungi
journal, April 1996

Liver transplantation represents the optimal treatment for fulminant hepatic failure from amanita phalloides poisoning
journal, April 2006

The prolyl oligopeptidase family
journal, February 2002

cDNAs encoding [D-Ala2] deltorphin precursors from skin of Phyllomedusa bicolor also contain genetic information for three dermorphin-related opioid peptides
journal, June 1990
  • Richter, K.; Egger, R.; Negri, L.
  • Proceedings of the National Academy of Sciences, Vol. 87, Issue 12, p. 4836-4839
  • DOI: 10.1073/pnas.87.12.4836

Microcin J25 Has a Threaded Sidechain-to-Backbone Ring Structure and Not a Head-to-Tail Cyclized Backbone
journal, October 2003
  • Rosengren, K. Johan; Clark, Richard J.; Daly, Norelle L.
  • Journal of the American Chemical Society, Vol. 125, Issue 41, p. 12464-12474
  • DOI: 10.1021/ja0367703

Ab initio Gene Finding in Drosophila Genomic DNA
journal, April 2000
  • Salamov, Asaf A.; Solovyev, Victor V.
  • Genome Research, Vol. 10, Issue 4, p. 516-522
  • DOI: 10.1101/gr.10.4.516

An Asparaginyl Endopeptidase Mediates in Vivo Protein Backbone Cyclization
journal, August 2007
  • Saska, Ivana; Gillon, Amanda D.; Hatsugai, Noriyuki
  • Journal of Biological Chemistry, Vol. 282, Issue 40, p. 29721-29728
  • DOI: 10.1074/jbc.M705185200

Highly-efficient transformation of the homobasidiomycete Schizophyllum commune to phleomycin resistance
journal, August 1994
  • Schuren, Frank H. J.; Wessels, Joseph G. H.
  • Current Genetics, Vol. 26, Issue 2, p. 179-183
  • DOI: 10.1007/BF00313808

Identification and Analysis of Multivalent Proteolytically Resistant Peptides from Gluten:  Implications for Celiac Sprue
journal, October 2005
  • Shan, Lu; Qiao, Shuo-Wang; Arentz-Hansen, Helene
  • Journal of Proteome Research, Vol. 4, Issue 5, p. 1732-1741
  • DOI: 10.1021/pr050173t

Structural Basis for Gluten Intolerance in Celiac Sprue
journal, September 2002
  • Shan, Lu; Molberg, Oyvind; Parrot, Isabelle
  • Science, Vol. 297, Issue 5590, p. 2275-2279
  • DOI: 10.1126/science.1074129

The use of heparin as a simple cost-effective means of controlling background In nudeic acid hybridization procedures
journal, January 1984

Substrate- and pH-dependent contribution of oxyanion binding site to the catalysis of prolyl oligopeptidase, a paradigm of the serine oligopeptidase family
journal, January 2000
  • Szeltner, Zoltan; Renner, Veronika; Polgar, Laszlo
  • Protein Science, Vol. 9, Issue 2, p. 353-360
  • DOI: 10.1110/ps.9.2.353

The Noncatalytic β-Propeller Domain of Prolyl Oligopeptidase Enhances the Catalytic Capability of the Peptidase Domain
journal, March 2000
  • Szeltner, Zoltan; Renner, Veronika; Polgar, Laszlo
  • Journal of Biological Chemistry, Vol. 275, Issue 20, p. 15000-15005
  • DOI: 10.1074/jbc.M000942200

Circular proteins — no end in sight
journal, March 2002

Xerocomus chrysenteron lectin: identification of a new pesticidal protein
journal, June 2003
  • Trigueros, Veronique; Lougarre, Andree; Ali-Ahmed, Djamila
  • Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1621, Issue 3, p. 292-298
  • DOI: 10.1016/S0304-4165(03)00098-9

Characterization of the Final Two Genes of the Gibberellin Biosynthesis Gene Cluster of Gibberella fujikuroi
journal, May 2003
  • Tudzynski, Bettina; Mihlan, Martina; Rojas, Maria Cecilia
  • Journal of Biological Chemistry, Vol. 278, Issue 31, p. 28635-28643
  • DOI: 10.1074/jbc.M301927200

Gibberellin Biosynthetic Pathway in Gibberella fujikuroi: Evidence for a Gene Cluster
journal, December 1998
  • Tudzynski, Bettina; Hölter, Katja
  • Fungal Genetics and Biology, Vol. 25, Issue 3, p. 157-170
  • DOI: 10.1006/fgbi.1998.1095

Occurrence of Amanita Toxins in American Collections of Deadly Amanitas
journal, June 1966
  • Tyler, V. E.; Benedict, R. G.; Brady, L. R.
  • Journal of Pharmaceutical Sciences, Vol. 55, Issue 6, p. 590-593
  • DOI: 10.1002/jps.2600550612

The role of enhancers in the regulation of cell-type-specific transcriptional control
journal, July 1986
  • Voss, Stephan D.; Schlokat, Uwe; Gruss, Peter
  • Trends in Biochemical Sciences, Vol. 11, Issue 7, p. 287-289
  • DOI: 10.1016/0968-0004(86)90031-9

Horizontal Gene Transfer and the Evolution of Secondary Metabolite Gene Clusters in Fungi: An Hypothesis
journal, August 2000

Ribosomal biosynthesis of the cyclic peptide toxins of Amanita mushrooms
journal, January 2010
  • Walton, Jonathan D.; Hallen-Adams, Heather E.; Luo, Hong
  • Biopolymers, Vol. 94, Issue 5, p. 659-664
  • DOI: 10.1002/bip.21416

Molecular phylogenetic studies in the genus Amanita
journal, July 1998
  • Weiß, Michael; Yang, Zhu-Liang; Oberwinkler, Franz
  • Canadian Journal of Botany, Vol. 76, Issue 7, p. 1170-1179
  • DOI: 10.1139/b98-129

Characterization of the ferrichrome A biosynthetic gene cluster in the homobasidiomycete Omphalotus olearius
journal, August 2005

Loss of a prolyl oligopeptidase confers resistance to lithium by elevation of inositol (1,4,5) trisphosphate
journal, May 1999
  • Williams, R. S. B.; Eames, M.; Ryves, W. J.
  • The EMBO Journal, Vol. 18, Issue 10, p. 2734-2745
  • DOI: 10.1093/emboj/18.10.2734

Pharmacogenetics in model systems: Defining a common mechanism of action for mood stabilisers
journal, July 2005
  • Williams, Robin S.B.
  • Progress in Neuro-Psychopharmacology and Biological Psychiatry, Vol. 29, Issue 6, p. 1029-1037
  • DOI: 10.1016/j.pnpbp.2005.03.020

A Draft Sequence of the Rice Genome (Oryza sativa L. ssp. indica)
journal, April 2002

Production and characterization of Amanitin toxins from a pure culture of Amanita exitialis
journal, November 2005