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Title: DHAD variants and methods of screening

Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
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OSTI Identifier:
Butamax Advanced Biofuels LLC ARPA-E
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Resource Relation:
Patent File Date: 2014 Mar 13
Research Org:
Butamax Advanced Biofuels LLC Wilmington, DE (United States)
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Country of Publication:
United States

Works referenced in this record:

Analysis of a 108-kb Region of the Saccharopolyspora spinosa Genome Covering the Obscurin Polyketide Synthase Locus
journal, April 2004

An Investigation of the Metabolism of Valine to Isobutyl Alcohol in Saccharomyces cerevisiae
journal, October 1998
  • Dickinson, J. Richard; Harrison, Scott J.; Hewlins, Michael J. E.
  • Journal of Biological Chemistry, Vol. 273, Issue 40, p. 25751-25756
  • DOI: 10.1074/jbc.273.40.25751

New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation
journal, June 1998
  • Dürre, P.
  • Applied Microbiology and Biotechnology, Vol. 49, Issue 6, p. 639-648
  • DOI: 10.1007/s002530051226

Involvement of branched-chain amino acid aminotransferases in the production of fusel alcohols during fermentation in yeast
journal, April 2001
  • Eden, A.; Van Nedervelde, L.; Drukker, M.
  • Applied Microbiology and Biotechnology, Vol. 55, Issue 3, p. 296-300
  • DOI: 10.1007/s002530000506

Studies on the Active Site of Dihydroxy-acid Dehydratase
journal, December 1993

Branched-chain amino acid biosynthesis genes in Lactococcus lactis subsp. lactis.
journal, October 1992

Technologies for butanol recovery integrated with fermentations
journal, March 1992
  • Groot, W. J.; van der Lans, R. G. J. M.; Luyben, K. Ch. A. M.
  • Process Biochemistry, Vol. 27, Issue 2, p. 61-75
  • DOI: 10.1016/0032-9592(92)80012-R

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension
journal, April 1989

Catalytic Promiscuity in Dihydroxy-Acid Dehydratase from the Thermoacidophilic Archaeon Sulfolobus solfataricus
journal, March 2006
  • Kim, Seonghun; Lee, Sun Bok
  • The Journal of Biochemistry, Vol. 139, Issue 3, p. 591-596
  • DOI: 10.1093/jb/mvj057

New thermosensitive plasmid for gram-positive bacteria.
journal, September 1992

High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening
journal, December 1993

Cloning of theILV2, ILV3 andILV5 genes of Saccharomyces cerevisiae
journal, January 1984
  • Polaina, Julio
  • Carlsberg Research Communications, Vol. 49, Issue 6, p. 577-584
  • DOI: 10.1007/BF02908686

Plasmid vectors for Gram-positive bacteria switching from high to low copy number
journal, January 1996

A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum
journal, April 2006

Electron spin relaxation of iron-sulphur proteins studied by microwave power saturation
journal, December 1978
  • Rupp, H.; Rao, K. K.; Hall, D. O.
  • Biochimica et Biophysica Acta (BBA) - Protein Structure, Vol. 537, Issue 2, p. 255-269
  • DOI: 10.1016/0005-2795(78)90509-3

Sequences of versatile broad-host-range vectors of the RK2 family
journal, July 2003

Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
journal, January 1994
  • Thompson, Julie D.; Higgins, Desmond G.; Gibson, Toby J.
  • Nucleic Acids Research, Vol. 22, Issue 22, p. 4673-4680
  • DOI: 10.1093/nar/22.22.4673

Functional Analysis of Three Plasmids from Lactobacillus plantarum
journal, March 2005

Heterologous protein production in yeast
book, January 1992

Industrial production of heterologous proteins by fed-batch cultures of the yeast Saccharomyces cereuisiae
journal, December 1994

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005
  • Chica, Roberto A.; Doucet, Nicolas; Pelletier, Joelle N.
  • Current Opinion in Biotechnology, Vol. 16, Issue 4, p. 378-384
  • DOI: 10.1016/j.copbio.2005.06.004

Cloning of the dihydroxyacid dehydratase-encoding gene (ILV3) from Saccharomyces cerevisiae
journal, December 1993

Disentangling the perturbational effects of amino acid substitutions in the DNA-binding domain of p53
journal, February 1999
  • Wacey, A. I.; Cooper, D. N.; Liney, D.
  • Human Genetics, Vol. 104, Issue 1, p. 15-22
  • DOI: 10.1007/s004390050904

Iron Acquisition and Transcriptional Regulation
journal, October 2009
  • Kaplan, Craig D.; Kaplan, Jerry
  • Chemical Reviews, Vol. 109, Issue 10, p. 4536-4552
  • DOI: 10.1021/cr9001676

Iron−Sulfur Cluster Biosynthesis: Functional Characterization of the N- and C-Terminal Domains of Human NFU
journal, February 2009
  • Liu, Yushi; Qi, Wenbin; Cowan, J. A.
  • Biochemistry, Vol. 48, Issue 5, p. 973-980
  • DOI: 10.1021/bi801645z

Codon usage tabulated from international DNA sequence databases: status for the year 2000
journal, January 2000
  • Nakamura, Yasukazu; Gojobori, Takashi; Ikemura, Toshimichi
  • Nucleic Acids Research, Vol. 28, Issue 1, p. 292-292
  • DOI: 10.1093/nar/28.1.292

Glutaredoxins Grx3 and Grx4 regulate nuclear localisation of Aft1 and the oxidative stress response in Saccharomyces cerevisiae
journal, November 2006
  • Pujol-Carrion, Nuria; Belli, Gemma; Herrero, Enrique
  • Journal of Cell Science, Vol. 119, Issue 21, p. 4554-4564
  • DOI: 10.1242/jcs.03229

New integration vector using a cellulase gene as a screening marker for Lactobacillus
journal, July 2003

Transformation of Lactobacillus plantarum by electroporation with in vitro modified plasmid DNA
journal, December 2004
  • Teresa Alegre, M.; Carmen Rodriguez, M.; Mesas, Juan M.
  • FEMS Microbiology Letters, Vol. 241, Issue 1, p. 73-77
  • DOI: 10.1016/j.femsle.2004.10.006