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Title: DHAD variants and methods of screening

Abstract

Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.

Inventors:
;
Issue Date:
Research Org.:
Butamax Advanced Biofuels LLC Wilmington, DE (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1345383
Patent Number(s):
9580705
Application Number:
14/207,823
Assignee:
Butamax Advanced Biofuels LLC
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AR0000006
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Mar 13
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Kelly, Kristen J., and Ye, Rick W. DHAD variants and methods of screening. United States: N. p., 2017. Web.
Kelly, Kristen J., & Ye, Rick W. DHAD variants and methods of screening. United States.
Kelly, Kristen J., and Ye, Rick W. Tue . "DHAD variants and methods of screening". United States. https://www.osti.gov/servlets/purl/1345383.
@article{osti_1345383,
title = {DHAD variants and methods of screening},
author = {Kelly, Kristen J. and Ye, Rick W.},
abstractNote = {Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {2}
}

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