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Title: DHAD variants and methods of screening

Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
Inventors:
;
Issue Date:
OSTI Identifier:
1345383
Assignee:
Butamax Advanced Biofuels LLC ARPA-E
Patent Number(s):
9,580,705
Application Number:
14/207,823
Contract Number:
AR0000006
Resource Relation:
Patent File Date: 2014 Mar 13
Research Org:
Butamax Advanced Biofuels LLC Wilmington, DE (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES

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