skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: DHAD variants and methods of screening

Abstract

Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.

Inventors:
;
Issue Date:
Research Org.:
Butamax Advanced Biofuels LLC Wilmington, DE (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1345383
Patent Number(s):
9580705
Application Number:
14/207,823
Assignee:
Butamax Advanced Biofuels LLC
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
DOE Contract Number:  
AR0000006
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Mar 13
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Kelly, Kristen J., and Ye, Rick W. DHAD variants and methods of screening. United States: N. p., 2017. Web.
Kelly, Kristen J., & Ye, Rick W. DHAD variants and methods of screening. United States.
Kelly, Kristen J., and Ye, Rick W. Tue . "DHAD variants and methods of screening". United States. https://www.osti.gov/servlets/purl/1345383.
@article{osti_1345383,
title = {DHAD variants and methods of screening},
author = {Kelly, Kristen J. and Ye, Rick W.},
abstractNote = {Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {2}
}

Patent:

Save / Share:

Works referenced in this record:

Analysis of a 108-kb Region of the Saccharopolyspora spinosa Genome Covering the Obscurin Polyketide Synthase Locus
journal, April 2004


An Investigation of the Metabolism of Valine to Isobutyl Alcohol in Saccharomyces cerevisiae
journal, October 1998


New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation
journal, June 1998


Involvement of branched-chain amino acid aminotransferases in the production of fusel alcohols during fermentation in yeast
journal, April 2001


Studies on the Active Site of Dihydroxy-acid Dehydratase
journal, December 1993


Branched-chain amino acid biosynthesis genes in Lactococcus lactis subsp. lactis.
journal, October 1992


Technologies for butanol recovery integrated with fermentations
journal, March 1992


Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension
journal, April 1989


Catalytic Promiscuity in Dihydroxy-Acid Dehydratase from the Thermoacidophilic Archaeon Sulfolobus solfataricus
journal, March 2006


New thermosensitive plasmid for gram-positive bacteria.
journal, September 1992


High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening
journal, December 1993


Cloning of theILV2, ILV3 andILV5 genes of Saccharomyces cerevisiae
journal, January 1984


Plasmid vectors for Gram-positive bacteria switching from high to low copy number
journal, January 1996


Electron spin relaxation of iron-sulphur proteins studied by microwave power saturation
journal, December 1978


Sequences of versatile broad-host-range vectors of the RK2 family
journal, July 2003


Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001


CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
journal, January 1994


Functional Analysis of Three Plasmids from Lactobacillus plantarum
journal, March 2005


Heterologous protein production in yeast
book, January 1992


Industrial production of heterologous proteins by fed-batch cultures of the yeast Saccharomyces cereuisiae
journal, December 1994


Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005


Cloning of the dihydroxyacid dehydratase-encoding gene (ILV3) from Saccharomyces cerevisiae
journal, December 1993


Disentangling the perturbational effects of amino acid substitutions in the DNA-binding domain of p53
journal, February 1999


Iron Acquisition and Transcriptional Regulation
journal, October 2009


Iron−Sulfur Cluster Biosynthesis: Functional Characterization of the N- and C-Terminal Domains of Human NFU
journal, February 2009


Codon usage tabulated from international DNA sequence databases: status for the year 2000
journal, January 2000


Glutaredoxins Grx3 and Grx4 regulate nuclear localisation of Aft1 and the oxidative stress response in Saccharomyces cerevisiae
journal, November 2006


New integration vector using a cellulase gene as a screening marker for Lactobacillus
journal, July 2003


Transformation of Lactobacillus plantarum by electroporation with in vitro modified plasmid DNA
journal, December 2004