Global to push GA events into
skip to main content

Title: Method for chemical amplification based on fluid partitioning in an immiscible liquid

A system for nucleic acid amplification of a sample comprises partitioning the sample into partitioned sections and performing PCR on the partitioned sections of the sample. Another embodiment of the invention provides a system for nucleic acid amplification and detection of a sample comprising partitioning the sample into partitioned sections, performing PCR on the partitioned sections of the sample, and detecting and analyzing the partitioned sections of the sample.
; ;
Issue Date:
OSTI Identifier:
Lawrence Livermore National Security, LLC LLNL
Patent Number(s):
Application Number:
Contract Number:
Resource Relation:
Patent File Date: 2015 Apr 30
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
Country of Publication:
United States

Other works cited in this record:

Apparatus for Dispensing Liquid Sample
patent, April 1971

Analysis system
patent, August 1981

Methods for isolating mutant microoganisms using microcapsules coated with indicator material
patent, January 1989

Capillary flow device
patent, August 1990

Apparatus for repeated automatic execution of a thermal cycle for treatment of samples
patent, January 1993

Device and method for the automated cycling of solutions between two or more temperatures
patent, December 1993

Microfluidic structure and process for its manufacture
patent, December 1994

Cell fixative composition and method of staining cells without destroying the cell surface
patent, June 1995

Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
patent, December 1996

Mesoscale polynucleotide amplification devices
patent, December 1996

Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
patent, February 1997

Inline thermo-cycler
patent, April 1998

Nucleic acid amplification reaction apparatus
patent, October 1998

Microfluidic systems incorporating varied channel dimensions
patent, December 1998

Integrated nucleic acid diagnostic device
patent, January 1999

Apparatus and method for performing electrodynamic focusing on a microchip
patent, January 1999

X-ray compass for determining device orientation
patent, June 1999

System for real time detection of nucleic acid amplification products
patent, July 1999

Apparatus for packaging a chip
patent, August 1999

Fluorescence monitoring device with textured optical tube and method for reducing background fluorescence
patent, October 1999

Microscale devices and reactions in microscale devices
patent, May 2000

Device for multiple analyte detection
patent, October 2000

Moving microdroplets
patent, October 2000

Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
patent, November 2000

Micromachined magnetohydrodynamic actuators and sensors
patent, November 2000

Controlled fluid transport microfabricated polymeric substrates
patent, December 2000

High throughput screening for novel enzymes
patent, January 2001

Optical coherence domain reflectometry guidewire
patent, January 2001

Magnetic tumble stirring method, devices and machines for mixing in vessels
patent, January 2001

Continuous manufacturing method for microspheres and apparatus
patent, January 2001

Method and apparatus for analysis and sorting of polynucleotides based on size
patent, April 2001

Microchannel apparatus and method of producing emulsions making use thereof
patent, August 2001

Microfluidic devices for electrophoretic analysis of materials
patent, January 2002

Methods for analysis and sorting of polynucleotides
patent, February 2002

Magnetic levitation stirring devices and machines for mixing in vessels
patent, March 2002

Catheter guided by optical coherence domain reflectometry
patent, May 2002

Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
patent, May 2002

Microfluidic systems and methods of genotyping
patent, June 2002

High-throughput screening assay systems in microscale fluidic devices
patent, August 2002

Digital amplification
patent, August 2002

Optical fiber head for providing lateral viewing
patent, October 2002

Pre-disposed assay components in microfluidic devices and methods
patent, November 2002

Multiplexed microfluidic devices, systems, and methods
patent, December 2002

Bend test for a wire harness and device for such a test
patent, December 2002

Microfluidic devices for the controlled manipulation of small volumes
patent, February 2003

Microfabricated cell sorter for chemical and biological materials
patent, April 2003

Device and method for the detection of an analyte utilizing mesoscale flow systems
patent, April 2003

Cell flow apparatus and method for real-time measurements of patient cellular responses
patent, May 2003

Methods and systems for fluid control in microfluidic devices
patent, June 2003

Coupling to microstructures for a laboratory microchip
patent, August 2003

Multi-channel microfluidic system design with balanced fluid flow distribution
patent, October 2003

Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
patent, December 2003

Method and systems for laser treatment of presbyopia using offset imaging
patent, December 2003

Method for conducting PCR protected from evaporation
patent, December 2003

Microfluidic devices and methods for performing temperature mediated reactions
patent, December 2003

Integrated active flux microfluidic devices and methods
patent, July 2004

Electrostatic actuators for microfluidics and methods for using same
patent, August 2004

Methods for detecting and sorting polynucleotides based on size
patent, December 2004

Microfluidic system and methods of use
patent, May 2005

Portable pathogen detection system
patent, June 2005

Nucleic acid amplification utilizing microfluidic devices
patent, November 2005

Methods for detecting nucleic acids indicative of cancer
patent, November 2005

Methods and systems for control of microfluidic devices
patent, March 2006

Chemical amplification based on fluid partitioning
patent, May 2006

Device for displacement of small liquid volumes along a micro-catenary line by electrostatic forces
patent, May 2006

Dual resonance energy transfer nucleic acid probes
patent, July 2006

High throughput screening assay systems in microscale fluidic devices
patent, August 2006

Device for parallel and synchronous injection for sequential injection of different reagents
patent, August 2006

Microfluidic device and methods of using same
patent, October 2006

Device and method for pressure-driven plug transport and reaction
patent, October 2006

Variable flexure-based fluid filter
patent, March 2007

Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids
patent, March 2007

patent, April 2007

Microfluidic devices for the controlled manipulation of small volumes
patent, July 2007

Double ended sequencing
patent, July 2007

In Vitro sorting method
patent, August 2007

Process for producing emulsion and microcapsules and apparatus therefor
patent, September 2007

Hydrophobic coating compositions, articles coated with said compositions, and processes for manufacturing same
patent, September 2007

Methods and systems for processing microfluidic samples of particle containing fluids
patent, September 2007

Crystal growth devices and systems, and methods for using same
patent, October 2007

Microfabricated crossflow devices and methods
patent, November 2007

Microfluidic particle-analysis systems
patent, December 2007

Methods of amplifying and sequencing nucleic acids
patent, January 2008

Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm
patent, May 2008

Miniaturized assembly and method of filling assembly
patent, December 2008

Microfluidic devices for controlled viscous shearing and formation of amphiphilic vesicles
patent, September 2009

Emulsion compositions
patent, November 2009

Assay apparatus and method using microfluidic arrays
patent, March 2010

Nucleic acid amplification using microfluidic devices
patent, November 2010

Bead emulsion nucleic acid amplification
patent, November 2010

Nucleic acid amplification with continuous flow emulsion
patent, April 2011

Method for detecting the presence of a single target nucleic acid in a sample
patent, July 2011

Methods of detecting amplified product
patent, November 2011

Method for detecting the presence of a single target nucleic acid in a sample
patent, September 2012

Method for detecting the presence of a single target nucleic acid in a sample
patent, October 2012

Thermocapillary valve for droplet production and sorting
journal, April 2007
  • Baroud, Charles N.; Delville, Jean-Pierre; Gallaire, Francois
  • Physical Review E, Vol. 75, Issue 4, Article No. 046302
  • DOI: 10.1103/PhysRevE.75.046302

On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets
journal, March 2008
  • Beer, N. Reginald; Wheeler, Elizabeth K.; Lee-Houghton, Lorenna
  • Analytical Chemistry, Vol. 80, Issue 6, p. 1854-1858
  • DOI: 10.1021/ac800048k

On-Chip, Real-Time, Single-Copy Polymerase Chain Reaction in Picoliter Droplets
journal, November 2007
  • Beer, N. Reginald; Hindson, Benjamin J.; Wheeler, Elizabeth K.
  • Analytical Chemistry, Vol. 79, Issue 22, p. 8471-8475
  • DOI: 10.1021/ac701809w

A microfluidic droplet generator based on a piezoelectric actuator
journal, January 2009
  • Bransky, Avishay; Korin, Natanel; Khoury, Maria
  • Lab Chip, Vol. 9, Issue 4, p. 516-520
  • DOI: 10.1039/B814810D

Droplet-Based Microfluidics for Emulsion and Solvent Evaporation Synthesis of Monodisperse Mesoporous Silica Microspheres
journal, February 2008
  • Carroll, Nick J.; Rathod, Shailendra B.; Derbins, Erin
  • Langmuir, Vol. 24, Issue 3, p. 658-661
  • DOI: 10.1021/la7032516

Droplet fusion by alternating current (AC) field electrocoalescence in microchannels
journal, October 2005
  • Chabert, Max; Dorfman, Kevin D.; Viovy, Jean-Louis
  • ELECTROPHORESIS, Vol. 26, Issue 19, p. 3706-3715
  • DOI: 10.1002/elps.200500109

Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms
journal, May 2008
  • Clausell-Tormos, Jenifer; Lieber, Diana; Baret, Jean-Christophe
  • Chemistry & Biology, Vol. 15, Issue 5, p. 427-437
  • DOI: 10.1016/j.chembiol.2008.04.004

Digital quantification of mutant DNA in cancer patients
journal, January 2007

Look before You Leap: Active Surveillance for Multidrug-Resistant Organisms
journal, April 2007
  • Weinstein, Robert A.; Diekema, Daniel J.; Edmond, Michael B.
  • Clinical Infectious Diseases, Vol. 44, Issue 8, p. 1101-1107
  • DOI: 10.1086/512820

Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations
journal, July 2003
  • Dressman, D.; Yan, H.; Traverso, G.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 15, p. 8817-8822
  • DOI: 10.1073/pnas.1133470100

Highly parallel genomic assays
journal, August 2006
  • Fan, Jian-Bing; Chee, Mark S.; Gunderson, Kevin L.
  • Nature Reviews Genetics, Vol. 7, Issue 8, p. 632-644
  • DOI: 10.1038/nrg1901

Coupling Microdroplet Microreactors with Mass Spectrometry: Reading the Contents of Single Droplets Online
journal, May 2009
  • Fidalgo, Luis M.; Whyte, Graeme; Ruotolo, Brandon T.
  • Angewandte Chemie International Edition, Vol. 48, Issue 20, p. 3665-3668
  • DOI: 10.1002/anie.200806103

Nanoliter scale PCR with TaqMan detection
journal, May 1997
  • Kalinina, Olga; Lebedeva, Irina; Brown, James
  • Nucleic Acids Research, Vol. 25, Issue 10, p. 1999-2004
  • DOI: 10.1093/nar/25.10.1999

PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets
journal, September 2005
  • Kojima, Takaaki; Takei, Yoshiaki; Ohtsuka, Miharu
  • Nucleic Acids Research, Vol. 33, Issue 17, p. e150-e150
  • DOI: 10.1093/nar/gni143

Chemical Amplification: Continuous-Flow PCR on a Chip
journal, May 1998

High-Throughput Single Copy DNA Amplification and Cell Analysis in Engineered Nanoliter Droplets
journal, May 2008
  • Kumaresan, Palani; Yang, Chaoyong James; Cronier, Samantha A.
  • Analytical Chemistry, Vol. 80, Issue 10, p. 3522-3529
  • DOI: 10.1021/ac800327d

Droplet Formation Utilizing Controllable Moving-Wall Structures for Double-Emulsion Applications
journal, June 2008
  • Lin, Yen-Heng; Lee, Chun-Hong; Lee, Gwo-Bin
  • Journal of Microelectromechanical Systems, Vol. 17, Issue 3, p. 573-581
  • DOI: 10.1109/JMEMS.2008.924273

Electric Control of Droplets in Microfluidic Devices
journal, April 2006
  • Link, Darren R.; Grasland-Mongrain, Erwan; Duri, Agnes
  • Angewandte Chemie International Edition, Vol. 45, Issue 16, p. 2556-2560
  • DOI: 10.1002/anie.200503540

Droplet-based synthetic method using microflow focusing and droplet fusion
journal, September 2006
  • Liu, Kan; Ding, Huijiang; Chen, Yong
  • Microfluidics and Nanofluidics, Vol. 3, Issue 2, p. 239-243
  • DOI: 10.1007/s10404-006-0121-8

Digital PCR for the molecular detection of fetal chromosomal aneuploidy
journal, July 2007
  • Lo, Y. M. D.; Lun, F. M. F.; Chan, K. C. A.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 32, p. 13116-13121
  • DOI: 10.1073/pnas.0705765104

Development of A Microchamber Array for Picoliter PCR
journal, March 2001
  • Nagai, Hidenori; Murakami, Yuji; Morita, Yasutaka
  • Analytical Chemistry, Vol. 73, Issue 5, p. 1043-1047
  • DOI: 10.1021/ac000648u

Mathematics of quantitative kinetic PCR and the application of standard curves
journal, August 2003
  • Rutledge, R. G.; Cote, C.
  • Nucleic Acids Research, Vol. 31, Issue 16, p. 93e-93
  • DOI: 10.1093/nar/gng093

Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications
journal, January 2004
  • Rutledge, R. G.
  • Nucleic Acids Research, Vol. 32, Issue 22, p. e178-e178
  • DOI: 10.1093/nar/gnh177

Amplification of complex gene libraries by emulsion PCR
journal, July 2006
  • Williams, Richard; Peisajovich, Sergio G.; Miller, Oliver J.
  • Nature Methods, Vol. 3, Issue 7, p. 545-550
  • DOI: 10.1038/nmeth896

Behavioral Modeling and Performance Evaluation of Microelectrofluidics-Based PCR Systems Using SystemC
journal, June 2004
  • Zhang, Tianhao; Chakrabarty, K.; Fair, R. B.
  • IEEE Transactions on Computer-Aided Design of Integrated Circuits and Systems, Vol. 23, Issue 6, p. 843-858
  • DOI: 10.1109/TCAD.2004.828115

Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends
journal, June 2007
  • Zhang, Chunsun; Xing, Da
  • Nucleic Acids Research, Vol. 35, Issue 13, p. 4223-4237
  • DOI: 10.1093/nar/gkm389

Microparticle Concentration and Separation by Traveling-Wave Dielectrophoresis (twDEP) for Digital Microfluidics
journal, December 2007
  • Zhao, Yuejun; Yi, Ui-Chong; Cho, Sung Kwon
  • Journal of Microelectromechanical Systems, Vol. 16, Issue 6, p. 1472-1481
  • DOI: 10.1109/JMEMS.2007.906763

Heat integration in micro-fluidic devices
book, January 2006

Similar records in DOepatents and OSTI.GOV collections: