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Title: Metabolic engineering of the shikimate pathway

The present disclosure relates to engineered microorganisms that produce amino acids and amino acid intermediates. In particular, the disclosure relates to recombinant nucleic acids encoding operons that increase production of aromatic amino acids and the aromatic amino acid intermediate shikimate; microorganisms with increased production of aromatic amino acids and the aromatic amino acid intermediate shikimate; and methods related to the production of aromatic amino acids, the aromatic amino acid intermediate shikimate, and commodity chemicals derived therefrom.
Inventors:
;
Issue Date:
OSTI Identifier:
1338929
Assignee:
The Regents of the University of California (Oakland, CA) LBNL
Patent Number(s):
9,540,652
Application Number:
14/342,335
Contract Number:
AC02-05CH11231
Resource Relation:
Patent File Date: 2012 Aug 31
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

Towards bacterial strains overproducing l-tryptophan and other aromatics by metabolic engineering
journal, December 2005

Metabolic engineering for microbial production of shikimic acid
journal, October 2003
  • Krämer, Marco; Bongaerts, Johannes; Bovenberg, Roel
  • Metabolic Engineering, Vol. 5, Issue 4, p. 277-283
  • DOI: 10.1016/j.ymben.2003.09.001

Modulation of Phosphoenolpyruvate Synthase Expression Increases Shikimate Pathway Product Yields in E. coli
journal, December 2002
  • Yi, J.; Li, K.; Draths, K.M.
  • Biotechnology Progress, Vol. 18, Issue 6, p. 1141-1148
  • DOI: 10.1021/bp020101w

Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine
journal, January 2007
  • Báez-Viveros, José; Flores, Noemí; Juárez, Katy
  • Microbial Cell Factories, Vol. 6, Issue 1, Article No. 30
  • DOI: 10.1186/1475-2859-6-30

Phosphoenolpyruvate Availability and the Biosynthesis of Shikimic Acid
journal, June 2003
  • Chandran, S.S.; Yi, J.; Draths, K.M.
  • Biotechnology Progress, Vol. 19, Issue 3, p. 808-814
  • DOI: 10.1021/bp025769p

Deletion of the aroK gene is essential for high shikimic acid accumulation through the shikimate pathway in E. coli
journal, September 2012

Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system
journal, January 2010
  • Escalante, Adelfo; Calderón, Rocío; Valdivia, Araceli
  • Microbial Cell Factories, Vol. 9, Issue 1, Article No. 21
  • DOI: 10.1186/1475-2859-9-21

Production of aromatic compounds in bacteria
journal, December 2009

Shikimic acid production by a modified strain of E. coli (W3110.shik1) under phosphate-limited and carbon-limited conditions
journal, January 2005
  • Johansson, Louise; Lindskog, Anna; Silfversparre, Gustav
  • Biotechnology and Bioengineering, Vol. 92, Issue 5, p. 541-552
  • DOI: 10.1002/bit.20546

Modular Engineering of l-Tyrosine Production in Escherichia coli
journal, October 2011
  • Juminaga, Darmawi; Baidoo, Edward E. K.; Redding-Johanson, Alyssa M.
  • Applied and Environmental Microbiology, Vol. 78, Issue 1, p. 89-98
  • DOI: 10.1128/AEM.06017-11

Combinatorial pathway analysis for improved L-tyrosine production in Escherichia coli: Identification of enzymatic bottlenecks by systematic gene overexpression
journal, March 2008

L-Tyrosine production by deregulated strains of Escherichia coli
journal, January 2007
  • Lütke-Eversloh, Tina; Stephanopoulos, Gregory
  • Applied Microbiology and Biotechnology, Vol. 75, Issue 1, p. 103-110
  • DOI: 10.1007/s00253-006-0792-9

Pathway engineering for production of aromatics in Escherichia coli: Confirmation of stoichiometric analysis by independent modulation of AroG, TktA, and Pps activities
journal, May 1995
  • Patnaik, Ranjan; Spitzer, Richard G.; Liao, James C.
  • Biotechnology and Bioengineering, Vol. 46, Issue 4, p. 361-370
  • DOI: 10.1002/bit.260460409

Ensemble Modeling for Aromatic Production in Escherichia coli
journal, September 2009

Altered Glucose Transport and Shikimate Pathway Product Yields in E. coli
journal, October 2003
  • Yi, J.; Draths, K.M.; Li, K.
  • Biotechnology Progress, Vol. 19, Issue 5, p. 1450-1459
  • DOI: 10.1021/bp0340584