Title: Metabolic engineering of the shikimate pathway

Abstract

The present disclosure relates to engineered microorganisms that produce amino acids and amino acid intermediates. In particular, the disclosure relates to recombinant nucleic acids encoding operons that increase production of aromatic amino acids and the aromatic amino acid intermediate shikimate; microorganisms with increased production of aromatic amino acids and the aromatic amino acid intermediate shikimate; and methods related to the production of aromatic amino acids, the aromatic amino acid intermediate shikimate, and commodity chemicals derived therefrom.

Inventors:

Juminaga, Darmawi; Keasling, Jay D.

Issue Date:

2017-01-10

Research Org.:

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1338929

Patent Number(s):

9540652

Application Number:

14/342,335

Assignee:

The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/52 - Genes encoding for enzymes or proenzymes
C12N15/70 - Vectors or expression systems specially adapted for E. coli
C12N9/001 - {acting on the CH-CH group of donors
C12N9/88 - Lyases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P13/22 - Tryptophan
C12P13/225 - {Tyrosine
C12P13/227 - {Tryptophan}
C12P7/42 - Hydroxy-carboxylic acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y103/01012 - Prephenate dehydrogenase
C12Y401/02 - Aldehyde-lyases

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

Y02P20/52 - using catalysts, e.g. selective catalysts

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DOE Contract Number:  

AC02-05CH11231

Resource Type:

Patent

Resource Relation:

Patent File Date: 2012 Aug 31

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES