Title: Methods for high yield production of terpenes

Abstract

Provided are enhanced high yield production systems for producing terpenes in plants via the expression of fusion proteins comprising various combinations of geranyl diphosphate synthase large and small subunits and limonene synthases. Also provided are engineered oilseed plants that accumulate monoterpene and sesquiterpene hydrocarbons in their seeds, as well as methods for producing such plants, providing a system for rapidly engineering oilseed crop production platforms for terpene-based biofuels.

Inventors:

Kutchan, Toni; Higashi, Yasuhiro; Feng, Xiaohong

Issue Date:

2017-01-03

Research Org.:

Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1338062

Patent Number(s):

9534227

Application Number:

13/893,105

Assignee:

Donald Danforth Plant Science Center (St. Louis, MO)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

A - HUMAN NECESSITIES A01 - AGRICULTURE A01H - NEW PLANTS OR PROCESSES FOR OBTAINING THEM

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/1085 - {transferring alkyl or aryl groups other than methyl groups

A - HUMAN NECESSITIES A01 - AGRICULTURE A01H - NEW PLANTS OR PROCESSES FOR OBTAINING THEM

A01H5/10 - Seeds

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K2319/08 - containing a chloroplats localisation signal

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07C - ACYCLIC OR CARBOCYCLIC COMPOUNDS

C07C2601/16 - the ring being unsaturated

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/8243 - {involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine}

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07C - ACYCLIC OR CARBOCYCLIC COMPOUNDS

C07C11/02 - Alkenes

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/88 - Lyases

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07C - ACYCLIC OR CARBOCYCLIC COMPOUNDS

C07C13/47 - with a bicyclo ring system containing ten carbon atoms
C07C13/20 - with a cyclohexene ring
C07C11/21 - Alkatrienes

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/8257 - {for the production of primary gene products, e.g. pharmaceutical products, interferon}

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07C - ACYCLIC OR CARBOCYCLIC COMPOUNDS

C07C15/02 - Monocyclic hydrocarbons

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DOE Contract Number:  

SC0001295

Resource Type:

Patent

Resource Relation:

Patent File Date: 2013 May 13

Country of Publication:

United States

Language:

English

Subject:

09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES