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Title: Miniature acoustic wave lysis system and uses thereof

The present invention relates to an acoustic lysis system including a disposable cartridge that can be reversibly coupled to a platform having a small, high-frequency piezoelectric transducer array. In particular, the system releases viable DNA, RNA, and proteins from human or bacterial cells, without chemicals or additional processing, to enable high-speed sample preparation for clinical point-of-care medical diagnostics and use with nano/microfluidic cartridges. Also described herein are methods of making and using the system of the invention.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1334665
Assignee:
Sandia Corporation (Albuquerque, NM) SNL-A
Patent Number(s):
9,512,421
Application Number:
14/318,364
Contract Number:
AC04-94AL85000
Resource Relation:
Patent File Date: 2014 Jun 27
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Other works cited in this record:

Nucleic acid purification using microfabricated silicon structures
journal, October 2003

Extensively drug-resistant tuberculosis as a cause of death in patients co-infected with tuberculosis and HIV in a rural area of South Africa
journal, November 2006

Reversible capture of genomic DNA by a Nafion-coated electrode
journal, September 2008
  • Lee, Myoyong; Lee, Jung-Nam; Kim, Jintae
  • Analytical Biochemistry, Vol. 380, Issue 2, p. 335-337
  • DOI: 10.1016/j.ab.2008.06.007

Nano/Microfluidics for diagnosis of infectious diseases in developing countries
journal, March 2010
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  • Advanced Drug Delivery Reviews, Vol. 62, Issue 4-5, p. 449-457
  • DOI: 10.1016/j.addr.2009.11.016

Microfluidic sonicator for real-time disruption of eukaryotic cells and bacterial spores for DNA analysis
journal, September 2005
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  • DOI: 10.1016/j.ultrasmedbio.2005.05.005

Rapid culture-based methods for drug-resistance detection in Mycobacterium tuberculosis
journal, October 2008
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  • DOI: 10.1016/j.mimet.2008.06.015

Shaping acoustic fields as a toolset for microfluidic manipulations in diagnostic technologies
journal, September 2012
  • Reboud, J.; Bourquin, Y.; Wilson, R.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 38, p. 15162-15167
  • DOI: 10.1073/pnas.1206055109

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