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Title: Compositions and methods for detecting single nucleotide polymorphisms

Abstract

Described herein are nucleic acid based probes and methods for discriminating and detecting single nucleotide variants in nucleic acid molecules (e.g., DNA). The methods include use of a pair of probes can be used to detect and identify polymorphisms, for example single nucleotide polymorphism in DNA. The pair of probes emit a different fluorescent wavelength of light depending on the association and alignment of the probes when hybridized to a target nucleic acid molecule. Each pair of probes is capable of discriminating at least two different nucleic acid molecules that differ by at least a single nucleotide difference. The methods can probes can be used, for example, for detection of DNA polymorphisms that are indicative of a particular disease or condition.

Inventors:
; ;
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1333288
Patent Number(s):
9,499,866
Application Number:
14/284,776
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
DOE Contract Number:  
AC52-06NA25396
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 May 22
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Yeh, Hsin-Chih, Werner, James, and Martinez, Jennifer S. Compositions and methods for detecting single nucleotide polymorphisms. United States: N. p., 2016. Web.
Yeh, Hsin-Chih, Werner, James, & Martinez, Jennifer S. Compositions and methods for detecting single nucleotide polymorphisms. United States.
Yeh, Hsin-Chih, Werner, James, and Martinez, Jennifer S. Tue . "Compositions and methods for detecting single nucleotide polymorphisms". United States. https://www.osti.gov/servlets/purl/1333288.
@article{osti_1333288,
title = {Compositions and methods for detecting single nucleotide polymorphisms},
author = {Yeh, Hsin-Chih and Werner, James and Martinez, Jennifer S.},
abstractNote = {Described herein are nucleic acid based probes and methods for discriminating and detecting single nucleotide variants in nucleic acid molecules (e.g., DNA). The methods include use of a pair of probes can be used to detect and identify polymorphisms, for example single nucleotide polymorphism in DNA. The pair of probes emit a different fluorescent wavelength of light depending on the association and alignment of the probes when hybridized to a target nucleic acid molecule. Each pair of probes is capable of discriminating at least two different nucleic acid molecules that differ by at least a single nucleotide difference. The methods can probes can be used, for example, for detection of DNA polymorphisms that are indicative of a particular disease or condition.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {11}
}

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