skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Compositions and methods for detecting single nucleotide polymorphisms

Abstract

Described herein are nucleic acid based probes and methods for discriminating and detecting single nucleotide variants in nucleic acid molecules (e.g., DNA). The methods include use of a pair of probes can be used to detect and identify polymorphisms, for example single nucleotide polymorphism in DNA. The pair of probes emit a different fluorescent wavelength of light depending on the association and alignment of the probes when hybridized to a target nucleic acid molecule. Each pair of probes is capable of discriminating at least two different nucleic acid molecules that differ by at least a single nucleotide difference. The methods can probes can be used, for example, for detection of DNA polymorphisms that are indicative of a particular disease or condition.

Inventors:
; ;
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1333288
Patent Number(s):
9499866
Application Number:
14/284,776
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC52-06NA25396
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 May 22
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Yeh, Hsin-Chih, Werner, James, and Martinez, Jennifer S. Compositions and methods for detecting single nucleotide polymorphisms. United States: N. p., 2016. Web.
Yeh, Hsin-Chih, Werner, James, & Martinez, Jennifer S. Compositions and methods for detecting single nucleotide polymorphisms. United States.
Yeh, Hsin-Chih, Werner, James, and Martinez, Jennifer S. Tue . "Compositions and methods for detecting single nucleotide polymorphisms". United States. https://www.osti.gov/servlets/purl/1333288.
@article{osti_1333288,
title = {Compositions and methods for detecting single nucleotide polymorphisms},
author = {Yeh, Hsin-Chih and Werner, James and Martinez, Jennifer S.},
abstractNote = {Described herein are nucleic acid based probes and methods for discriminating and detecting single nucleotide variants in nucleic acid molecules (e.g., DNA). The methods include use of a pair of probes can be used to detect and identify polymorphisms, for example single nucleotide polymorphism in DNA. The pair of probes emit a different fluorescent wavelength of light depending on the association and alignment of the probes when hybridized to a target nucleic acid molecule. Each pair of probes is capable of discriminating at least two different nucleic acid molecules that differ by at least a single nucleotide difference. The methods can probes can be used, for example, for detection of DNA polymorphisms that are indicative of a particular disease or condition.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {11}
}

Patent:

Save / Share:

Works referenced in this record:

Silver(I) Complexes with DNA and RNA Studied by Fourier Transform Infrared Spectroscopy and Capillary Electrophoresis
journal, September 2001


Short-Range Spectroscopic Ruler Based on a Single-Molecule Optical Switch
journal, March 2005


Mutation detection by electrocatalysis at DNA-modified electrodes
journal, October 2000


DNA Nanoswitch as a Biosensor
journal, June 2007


Rapid genotyping of single nucleotide polymorphisms using novel minor groove binding DNA oligonucleotides (MGB probes)
journal, April 2002


Fluorescent silver nanoclusters
journal, January 2011


Drivers of biodiagnostic development
journal, November 2009


Highly Sequence-Dependent Formation of Fluorescent Silver Nanoclusters in Hybridized DNA Duplexes for Single Nucleotide Mutation Identification
journal, January 2010


Sequence-Dependent Fluorescence of DNA-Hosted Silver Nanoclusters
journal, January 2008


Photoinduced Electron Transfer between Fluorescent Dyes and Guanosine Residues in DNA-Hairpins
journal, August 2003


Single-nucleotide polymorphism detection with "wire-like" DNA probes that display quasi "on-off" digital action
journal, August 2005


Binary Malachite Green Aptamer for Fluorescent Detection of Nucleic Acids
journal, September 2005


Molecular Beacons: Spectral Genotyping of Human Alleles
journal, February 1998


Pathogenesis of Ovarian Cancer: Lessons From Morphology and Molecular Biology and Their Clinical Implications
journal, January 2008


M ETHODS FOR G ENOTYPING S INGLE N UCLEOTIDE P OLYMORPHISMS
journal, September 2001


Strongly enhanced field-dependent single-molecule electroluminescence
journal, July 2002


Using Molecular Beacons to Detect Single-Nucleotide Polymorphisms with Real-Time PCR
journal, December 2001


Ag K-Edge EXAFS Analysis of DNA-Templated Fluorescent Silver Nanoclusters: Insight into the Structural Origins of Emission Tuning by DNA Sequence Variations
journal, August 2011


Pyrene-Labeled Base-Discriminating Fluorescent DNA Probes for Homogeneous SNP Typing
journal, April 2004


Design of base-discriminating fluorescent nucleosides
journal, October 2005


UV Excitation of DNA Stabilized Ag Cluster Fluorescence via the DNA Bases
journal, November 2011


Hairpins with Poly-C Loops Stabilize Four Types of Fluorescent Ag n :DNA
journal, February 2009


DNA Sensing by Amplifying the Number of Near-Infrared Emitting, Oligonucleotide-Encapsulated Silver Clusters
journal, August 2011


DNA-Templated Ag Nanocluster Formation
journal, April 2004


Ag Nanocluster Formation Using a Cytosine Oligonucleotide Template
journal, January 2007


Silver nanocluster aptamers: in situ generation of intrinsically fluorescent recognition ligands for protein detection
journal, January 2011


A complementary palette of fluorescent silver nanoclusters
journal, January 2010


A molecular ruler based on plasmon coupling of single gold and silver nanoparticles
journal, May 2005


The properties of small Ag clusters bound to DNA bases
journal, May 2010


Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors
journal, January 2006


The Label-Free Unambiguous Detection and Symbolic Display of Single Nucleotide Polymorphisms on DNA Origami
journal, February 2011


Single-nucleotide mutation rate increases close to insertions/deletions in eukaryotes
journal, July 2008


Strongly emissive individual DNA-encapsulated Ag nanoclusters as single-molecule fluorophores
journal, May 2007


K-ras mutations in the duodenal fluid of patients with pancreatic carcinoma
journal, January 1998


Fluorescence Detection of Single-Nucleotide Polymorphisms with a Single, Self-Complementary, Triple-Stem DNA Probe
journal, June 2009


Tunable Blinking Kinetics of Cy5 for Precise DNA Quantification and Single-Nucleotide Difference Detection
journal, July 2008


A DNA−Silver Nanocluster Probe That Fluoresces upon Hybridization
journal, August 2010


A Beacon of Light
journal, June 2011


A Fluorescence Light-Up Ag Nanocluster Probe That Discriminates Single-Nucleotide Variants by Emission Color
journal, July 2012


Nanometal Surface Energy Transfer in Optical Rulers, Breaking the FRET Barrier
journal, March 2005


Single-nucleotide polymorphism genotyping on optical thin-film biosensor chips
journal, September 2003