Title: Compositions and methods for detecting single nucleotide polymorphisms

Abstract

Described herein are nucleic acid based probes and methods for discriminating and detecting single nucleotide variants in nucleic acid molecules (e.g., DNA). The methods include use of a pair of probes can be used to detect and identify polymorphisms, for example single nucleotide polymorphism in DNA. The pair of probes emit a different fluorescent wavelength of light depending on the association and alignment of the probes when hybridized to a target nucleic acid molecule. Each pair of probes is capable of discriminating at least two different nucleic acid molecules that differ by at least a single nucleotide difference. The methods can probes can be used, for example, for detection of DNA polymorphisms that are indicative of a particular disease or condition.

Inventors:

Yeh, Hsin-Chih; Werner, James; Martinez, Jennifer S.

Issue Date:

2016-11-22

Research Org.:

Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1333288

Patent Number(s):

9499866

Application Number:

14/284,776

Assignee:

Los Alamos National Security, LLC (Los Alamos, NM)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6827 - for detection of mutation or polymorphism
C12Q1/6881 - for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
C12Q1/6883 - for diseases caused by alterations of genetic material
C12Q2563/107 - fluorescence
C12Q2563/137 - Metal/ion, e.g. metal label
C12Q2563/155 - Particles of a defined size, e.g. nanoparticles
C12Q2600/156 - Polymorphic or mutational markers

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DOE Contract Number:  

AC52-06NA25396

Resource Type:

Patent

Resource Relation:

Patent File Date: 2014 May 22

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES