Title: Compositions and methods for increased ethanol titer from biomass

Abstract

The present application discloses the identification of novel I. orientalis ADH1, ADHa, and ADHb genes, and the production and characterization of genetically modified yeast cells in which these genes were altered. Provided herein are isolated I. orientalis ADH1, ADHa, and ADHb polynucleotides and polypeptides, genetically modified yeast cells that overexpress I. orientalis ADH1 and/or contain deletions or disruptions of ADHa and/or ADHb, and methods of using culturing these modified cells to produce ethanol.

Inventors:

Jessen, Holly J.; Yi, Jian

Issue Date:

2016-11-15

Research Org.:

Cargill, Incorporated, Wayzata, MN (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1332276

Patent Number(s):

9493793

Application Number:

13/988,899

Assignee:

Cargill, Incorporated (Wayzata, MN)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/0006 - {acting on CH-OH groups as donors

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/06 - Ethanol, i.e. non-beverage
C12P7/065 - {with microorganisms other than yeasts}
C12P7/10 - substrate containing cellulosic material

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

Y02P20/52 - using catalysts, e.g. selective catalysts

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DOE Contract Number:  

FC36-07GO17055

Resource Type:

Patent

Resource Relation:

Patent File Date: 2011 Nov 22

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS