System and method for measuring particles in a sample stream of a flow cytometer using low-power laser source
Abstract
A system and method for analyzing a particle in a sample stream of a flow cytometer or the like. The system has a light source, such as a laser pointer module, for generating a low powered light beam and a fluidics apparatus which is configured to transport particles in the sample stream at substantially low velocity through the light beam for interrogation. Detectors, such as photomultiplier tubes, are configured to detect optical signals generated in response to the light beam impinging the particles. Signal conditioning circuitry is connected to each of the detectors to condition each detector output into electronic signals for processing and is designed to have a limited frequency response to filter high frequency noise from the detector output signals.
- Inventors:
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1332118
- Patent Number(s):
- 9494509
- Application Number:
- 14/280,053
- Assignee:
- Los Alamos National Security, LLC (Los Alamos, NM)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC52-06NA25396
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2014 May 16
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Graves, Steven W., and Habbersett, Robert C. System and method for measuring particles in a sample stream of a flow cytometer using low-power laser source. United States: N. p., 2016.
Web.
Graves, Steven W., & Habbersett, Robert C. System and method for measuring particles in a sample stream of a flow cytometer using low-power laser source. United States.
Graves, Steven W., and Habbersett, Robert C. Tue .
"System and method for measuring particles in a sample stream of a flow cytometer using low-power laser source". United States. https://www.osti.gov/servlets/purl/1332118.
@article{osti_1332118,
title = {System and method for measuring particles in a sample stream of a flow cytometer using low-power laser source},
author = {Graves, Steven W. and Habbersett, Robert C.},
abstractNote = {A system and method for analyzing a particle in a sample stream of a flow cytometer or the like. The system has a light source, such as a laser pointer module, for generating a low powered light beam and a fluidics apparatus which is configured to transport particles in the sample stream at substantially low velocity through the light beam for interrogation. Detectors, such as photomultiplier tubes, are configured to detect optical signals generated in response to the light beam impinging the particles. Signal conditioning circuitry is connected to each of the detectors to condition each detector output into electronic signals for processing and is designed to have a limited frequency response to filter high frequency noise from the detector output signals.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {11}
}
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