Global to push GA events into
skip to main content

Title: System and method for measuring particles in a sample stream of a flow cytometer using low-power laser source

A system and method for analyzing a particle in a sample stream of a flow cytometer or the like. The system has a light source, such as a laser pointer module, for generating a low powered light beam and a fluidics apparatus which is configured to transport particles in the sample stream at substantially low velocity through the light beam for interrogation. Detectors, such as photomultiplier tubes, are configured to detect optical signals generated in response to the light beam impinging the particles. Signal conditioning circuitry is connected to each of the detectors to condition each detector output into electronic signals for processing and is designed to have a limited frequency response to filter high frequency noise from the detector output signals.
Issue Date:
OSTI Identifier:
Los Alamos National Security, LLC (Los Alamos, NM) LANL
Patent Number(s):
Application Number:
Contract Number:
Resource Relation:
Patent File Date: 2014 May 16
Research Org:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org:
Country of Publication:
United States

Other works cited in this record:

Design of Electro-Mechanical Transducer Elements
patent, August 1959

Liquid Borne Particle Sensor
patent, June 1972

Material suspension within an acoustically excited resonant chamber
patent, May 1975

Apparatus and method for removing fine particles from a liquid medium by ultrasonic waves
patent, October 1977

Remote acoustic wave sensors
patent, May 1981

Method and apparatus for field flow fractionation
patent, August 1981

Antibody production from hybrid cell line
patent, September 1982

Human nonsecretory plasmacytoid cell line
patent, February 1984

Method for discriminating between unstained and absorbing dye stained cells
patent, January 1985

Apparatus and method for regulating sheath fluid flow in a hydrodynamically focused fluid flow system
patent, March 1985

Acoustic particle separation
patent, June 1985

Electron rich aromatics as cure promoters for radiation curable compositions
patent, June 1985

Screening method for red cell abnormality
patent, June 1986

Broadband radial vibrator transducer with multiple resonant frequencies
patent, August 1986

Particle separation
patent, June 1987

Manipulation of particles
patent, May 1988

Methods and apparatus for moving and separating materials exhibiting different physical properties
patent, July 1988

Controlled sample orientation and rotation in an acoustic levitator
patent, October 1988

Housing for a flow cytometry apparatus with particle unclogging feature
patent, December 1988

Biological sample mixing apparatus and method
patent, July 1989

Liquid/liquid fiber-optic fluorescence detector and absorbance analyzer
patent, September 1989

Manipulating particulate matter
patent, October 1989

Particle agglutination immunoassay apparatus
patent, April 1990

Acoustic positioning and orientation prediction
patent, October 1990

High sensitivity fluorescent single particle and single molecule detection apparatus and method
patent, December 1990

Method for analysis of subpopulations of cells
patent, January 1991

Acousto-optic modulator for optical fibers using Hertzian contact with a grooved transducer substrate
patent, February 1991

Method and apparatus for sorting particles with a moving catcher tube
patent, July 1991

Chemiluminescence-based static and flow cytometry
patent, July 1991

Sheathed particle flow controlled by differential pressure
patent, August 1991

Analyzing system using sheath flow of sample
patent, January 1992

Acoustically driven particle separation method and apparatus
patent, February 1992

Method and apparatus for the indentification of particles
patent, April 1992

Process for the separation of substances from a liquid and device for effecting such a process
patent, November 1992

Method and apparatus for separating particles
patent, July 1993

Particle analyzing apparatus having pressure control system
patent, September 1993

Bioluminescence bathyphotometer
patent, November 1993

Phthalocyanine and tetrabenztriazaporphyrin reagents
patent, September 1994

Apparatus for using fluorescently labeled ligands in studying interaction of a native ligand and its receptor
patent, December 1994

Control of flow cytometer having vacuum fluidics
patent, March 1995

High efficiency fluorescence flow cell for capillary liquid chromatography or capillary electrophoresis
patent, July 1995

Method and system for examining the composition of a fluid or solid sample using fluorescence and/or absorption spectroscopy
patent, February 1996

Method and apparatus for performing phase fluorescence lifetime measurements in flow cytometry
patent, April 1996

Intra-liquid particle classification apparatus using light scattering
patent, May 1996

Multilayered piezoelectric resonator for the separation of suspended particles
patent, June 1996

Particle analyzer with spatially split wavelength filter
patent, June 1996

Apparatus and method for volumetric capillary cytometry
patent, August 1996

Acoustic filter for separating and recycling suspended particles
patent, May 1997

Imaging flow cytometer nearly simultaneously capturing a plurality of images
patent, July 1997

Up-converting reporters for biological and other assays using laser excitation techniques
patent, October 1997

Method and apparatus for separating particulate from a flowing fluid
patent, November 1997

Flow cell apparatus
patent, November 1997

Multilayered piezoelectric resonator for the separation of suspended particles
patent, January 1998

Liquid flow cytometer
patent, April 1998

Apparatus for monitoring substances in organisms
patent, August 1998

High solid infrared absorbing compositions
patent, September 1998

Method of non-contact micromanipulation using ultrasound
patent, November 1998

Pulseless liquid supply system for flow cytometry
patent, June 1999

Multiplexed analysis of clinical specimens apparatus and methods
patent, November 1999

System for manipulating drops and bubbles using acoustic radiation pressure
patent, December 1999

Capillary electrophoresis apparatus
patent, April 2000

Non-contact micromanipulation method and apparatus
patent, May 2000

Synthetic polymer particles for use as standards and calibrators in flow cytometry
patent, June 2000

Method and apparatus for acoustically demixing aqueous solutions
patent, July 2000

Method and apparatus for determining the state of fouling/cleaning of membrane modules
patent, December 2000

Method for enumerating blood cells
patent, March 2001

Particle handling apparatus for handling particles in fluid by acoustic radiation pressure
patent, April 2001

Method and apparatus for acoustically driven media filtration
patent, April 2001

Method and apparatus for analyzing cells in a whole blood sample
patent, May 2001

Method and apparatus for flow cytometry
patent, June 2001

Method for using an all solid-state fluorometer in industrial water system applications
patent, July 2001

High throughput analysis of samples in flowing liquid
patent, October 2001

Particle manipulation
patent, December 2001

Aerodynamic beam generator for large particles
patent, February 2002

Dispersive near-IR Raman spectrometer
patent, April 2002

Method and device for determining particle size distribution and zeta potential in concentrated dispersions
patent, September 2002

Cylindrical acoustic levitator/concentrator
patent, October 2002

Measuring the velocity of small moving objects such as cells
patent, March 2003

Optical detection system for flow cytometry
patent, April 2003

Actuators for microfluidics without moving parts
patent, May 2003

Focusing of microparticles in microfluidic systems
patent, July 2003

Low acceleration method of flow cytometry
patent, August 2003

Cylindrical acoustic levitator/concentrator having non-circular cross-section
patent, November 2003

Ice making machine
patent, November 2003

Ultrasonic flowmeter and manufacturing method thereof
patent, December 2003

Fluorescence detection instrument with reflective transfer legs for color decimation
patent, January 2004

Flow cytometer
patent, March 2004

Method and apparatus for the generation of ultrasonic energy fields within circular structures containing a liquid
patent, May 2004

Method for energy barrier equalization of magnetic recording media and media obtained thereby
patent, August 2004

Sensors and methods for high-sensitivity optical particle counting and sizing
patent, September 2004

Method and apparatus for acoustically driven media filtration
patent, September 2004

Apparatus and method employing incoherent light emitting semiconductor devices as particle detection light sources in a flow cytometer
patent, November 2004

Method and apparatus for detecting microparticles in fluid samples
patent, November 2004

Laser diode-excited biological particle detection system
patent, December 2004

Apparatuses and methods for field flow fractionation of particles using acoustic and other forces
patent, April 2005

Method of manufacturing a multi-layer semiconductor nanoparticle, and a multi-layer semiconductor nanoparticle manufactured by the method
patent, June 2005

Device and method for separation
patent, August 2005

Methods, compositions, and automated systems for separating rare cells from fluid samples
patent, September 2005

Nucleic acid sequencing by raman monitoring of molecular deconstruction
patent, January 2006

Ultrasonically cleaned membrane filtration system
patent, March 2006

Method and apparatus for manipulation of cells and cell-like structures focused electric fields in microfludic systems and use thereof
patent, March 2006

Ultrasonic monitor of material composition and particle size
patent, May 2006

Bioanalytical measuring method using oxidases and lanthanoid-ligand complexes
patent, May 2006

Methods for manipulating moieties in microfluidic systems
patent, July 2006

Fluorescent dyes, energy transfer couples and methods
patent, August 2006

Method and apparatus for separating particles by size
patent, September 2006

Flow cytometer for differentiating small particles in suspension
patent, September 2006

High resolution imaging fountain flow cytometry
patent, January 2007

Methods, compositions, and automated systems for separating rare cells from fluid samples
patent, January 2007

Method of using actuators for microfluidics without moving parts
patent, August 2007

Optical detection system for flow cytometry
patent, August 2007

Imaging and analyzing parameters of small moving objects such as cells
patent, January 2008

Methods for sampling a liquid flow
patent, February 2008

Ultrasonic analyte concentration and application in flow cytometry
patent, March 2008

Method and systems for dynamic range expansion
patent, April 2008

Apparatus for directing particles in a fluid
patent, May 2008

Flow cytometry system with bubble detection
patent, July 2008

Apparatus for sterilizing a liquid with focused acoustic standing waves
patent, October 2008

Flow cytometer
patent, January 2009

Compact efficient and robust ultraviolet solid-state laser sources based on nonlinear frequency conversion in periodically poled materials
patent, August 2009

System for analyzing particles using multiple flow cytometry units
patent, July 2010

Flow cytometer and flow cytometry
patent, August 2011

Particle imaging systems and methods using acoustic radiation pressure
patent, March 2012

Inorganic phosphors as new luminescent labels for immunocytochemistry and time-resolved microscopy
journal, January 1990
  • Beverloo, H. B.; van Schadewijk, A.; van Gelderen-Boele, S.
  • Cytometry, Vol. 11, Issue 7, p. 784-792
  • DOI: 10.1002/cyto.990110704

Microchip-Based Cell Lysis and DNA Extraction from Sperm Cells for Application to Forensic Analysis
journal, March 2006

Resolution of dimly fluorescent particles: A practical measure of fluorescence sensitivity
journal, October 1998

Impedance spectroscopy flow cytometry: On-chip label-free cell differentiation
journal, January 2005
  • Cheung, Karen; Gawad, Shady; Renaud, Philippe
  • Cytometry Part A, Vol. 65A, Issue 2, p. 124-132
  • DOI: 10.1002/cyto.a.20141

Time-resolved flow cytometry for the measurement of lanthanide chelate fluorescence: II. Instrument design and experimental results
journal, July 1994
  • Condrau, Marc A.; Schwendener, Reto A.; Zimmermann, Marcel
  • Cytometry, Vol. 16, Issue 3, p. 195-205
  • DOI: 10.1002/cyto.990160303

A Novel Ultrasonic Resonance Field Device for the Retention of Animal Cells
journal, July 1994
  • Doblhoff-Dier, O.; Gaida, Th.; Katinger, H.
  • Biotechnology Progress, Vol. 10, Issue 4, p. 428-432
  • DOI: 10.1021/bp00028a600

Acoustic Radiation Pressure on a Rigid Sphere in a Viscous Fluid
journal, December 1994
  • Doinikov, A. A.
  • Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 447, Issue 1931, p. 447-466
  • DOI: 10.1098/rspa.1994.0150

Major signal increase in fluorescence microscopy through dark-state relaxation
journal, December 2006
  • Donnert, Gerald; Eggeling, Christian; Hell, Stefan W.
  • Nature Methods, Vol. 4, Issue 1, p. 81-86
  • DOI: 10.1038/nmeth986

Classification of Spectroscopically Encoded Resins by Raman Mapping and Infrared Hyperspectral Imaging
journal, March 2006
  • Fenniri, Hicham; Terreau, Owen; Chun, Sangki
  • Journal of Combinatorial Chemistry, Vol. 8, Issue 2, p. 192-198
  • DOI: 10.1021/cc050128i

Selective Retention of Viable Cells in Ultrasonic Resonance Field Devices
journal, February 1996
  • Gaida, Th.; Doblhoff-Dier, O.; Strutzenberger, K.
  • Biotechnology Progress, Vol. 12, Issue 1, p. 73-76
  • DOI: 10.1021/bp950040k

Quantum Dot-Encoded Mesoporous Beads with High Brightness and Uniformity:  Rapid Readout Using Flow Cytometry
journal, April 2004
  • Gao, Xiaohu; Nie, Shuming
  • Analytical Chemistry, Vol. 76, Issue 8, p. 2406-2410
  • DOI: 10.1021/ac0354600

Single particle high resolution spectral analysis flow cytometry
journal, January 2006
  • Goddard, Gregory; Martin, John C.; Naivar, Mark
  • Cytometry Part A, Vol. 69A, Issue 8, p. 842-851
  • DOI: 10.1002/cyto.a.20320

Ultrasonic particle-concentration for sheathless focusing of particles for analysis in a flow cytometer
journal, January 2006
  • Goddard, Gregory; Martin, John C.; Graves, Steven W.
  • Cytometry Part A, Vol. 69A, Issue 2, p. 66-74
  • DOI: 10.1002/cyto.a.20205

Single-collector experiments and modeling of acoustically aided mesh filtration
journal, January 2005
  • Grossner, Michael T.; Feke, Donald L.; Belovich, Joanne M.
  • AIChE Journal, Vol. 51, Issue 6, p. 1590-1598
  • DOI: 10.1002/aic.10423

Positioning, displacement, and localization of cells using ultrasonic forces
journal, January 2005
  • Haake, Albrecht; Neild, Adrian; Radziwill, Gerald
  • Biotechnology and Bioengineering, Vol. 92, Issue 1, p. 8-14
  • DOI: 10.1002/bit.20540

Zeptomole detection sensitivity of prostate-specific antigen in a rapid microtitre plate assay using time-resolved fluorescence
journal, January 2000

Micromanipulation using a focused ultrasonic standing wave field
journal, January 2000

Manipulation of Fluid Objects with Acoustic Radiation Pressure
journal, January 2006
  • Marston, Philip L.; Thiessen, David B.
  • Annals of the New York Academy of Sciences, Vol. 1027, Issue 1, p. 414-434
  • DOI: 10.1196/annals.1324.034

Acoustic control of suspended particles in micro fluidic chips
journal, January 2004
  • Nilsson, Andreas; Petersson, Filip; Jönsson, Henrik
  • Lab Chip, Vol. 4, Issue 2, p. 131-135
  • DOI: 10.1039/B313493H

Carrier Medium Exchange through Ultrasonic Particle Switching in Microfluidic Channels
journal, March 2005
  • Petersson, Filip; Nilsson, Andreas; Jönsson, Henrik
  • Analytical Chemistry, Vol. 77, Issue 5, p. 1216-1221
  • DOI: 10.1021/ac048394q

Evaluation of SDF-1/CXCR4-induced Ca2+ signaling by fluorometric imaging plate reader (FLIPR) and flow cytometry
journal, December 2002
  • Princen, Katrien; Hatse, Sigrid; Vermeire, Kurt
  • Cytometry, Vol. 51A, Issue 1, p. 35-45
  • DOI: 10.1002/cyto.a.10008

Continuous Flow Microfluidic Device for Rapid Erythrocyte Lysis
journal, November 2004
  • Sethu, Palaniappan; Anahtar, Melis; Moldawer, Lyle L.
  • Analytical Chemistry, Vol. 76, Issue 21, p. 6247-6253
  • DOI: 10.1021/ac049429p

Individual Escherichia coli cells studied from light scattering with the scanning flow cytometer
journal, January 2000

New types of microspheres and microsphere-related materials for medical diagnostics
journal, October 2002
  • Slomkowski, Stanislaw; Basinska, Teresa; Miksa, Beata
  • Polymers for Advanced Technologies, Vol. 13, Issue 10-12, p. 905-918
  • DOI: 10.1002/pat.283

Dual-laser, differential fluorescence correction method for reducing cellular background autofluorescence
journal, November 1986

Data Analysis for a Dual-Channel Virus Counter
journal, April 2005
  • Stoffel, Carrie L.; Rowlen, Kathy L.
  • Analytical Chemistry, Vol. 77, Issue 7, p. 2243-2246
  • DOI: 10.1021/ac048626l

Diazo Coupling Method for Covalent Attachment of Proteins to Solid Substrates
journal, March 2006
  • Wu, Yang; Buranda, Tione; Metzenberg, Robert L.
  • Bioconjugate Chemistry, Vol. 17, Issue 2, p. 359-365
  • DOI: 10.1021/bc050278m

Flow cytometry to evaluate Theileria sergenti parasitemia using the fluorescent nucleic acid stain, SYTO16
journal, January 2000

Similar records in DOepatents and OSTI.GOV collections: