Title: Multiscale spectral nanoscopy

Abstract

A system and method for non-invasively tracking a particle in a sample is disclosed. The system includes a 2-photon or confocal laser scanning microscope (LSM) and a particle-holding device coupled to a stage with X-Y and Z position control. The system also includes a tracking module having a tracking excitation laser, X-Y and Z radiation-gathering components configured to detect deviations of the particle in an X-Y and Z directions. The system also includes a processor coupled to the X-Y and Z radiation gathering components, generate control signals configured to drive the stage X-Y and Z position controls to track the movement of the particle. The system may also include a synchronization module configured to generate LSM pixels stamped with stage position and a processing module configured to generate a 3D image showing the 3D trajectory of a particle using the LSM pixels stamped with stage position.

Inventors:

Yang, Haw; Welsher, Kevin

Issue Date:

Tue Nov 15 00:00:00 EST 2016

Research Org.:

Princeton Univ., NJ (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1332117

Patent Number(s):

9494518

Application Number:

14/415,779

Assignee:

THE TRUSTEES OF PRINCETON UNIVERSITY (Princeton, NJ)

Patent Classifications (CPCs):

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G - PHYSICS G01 - MEASURING G01Q - SCANNING-PROBE TECHNIQUES OR APPARATUS

Show more

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N15/1475 - {using image analysis for extracting features of the particle}
G01N21/6458 - {Fluorescence microscopy

G - PHYSICS G01 - MEASURING G01Q - SCANNING-PROBE TECHNIQUES OR APPARATUS

G01Q90/00 - Scanning-probe techniques or apparatus not otherwise provided for

G - PHYSICS G02 - OPTICS G02B - OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS

G02B21/0024 - {Confocal scanning microscopes

Show less

DOE Contract Number:  

SC0006838

Resource Type:

Patent

Resource Relation:

Patent File Date: 2013 Jul 18

Country of Publication:

United States

Language:

English

Subject:

47 OTHER INSTRUMENTATION; 42 ENGINEERING; 59 BASIC BIOLOGICAL SCIENCES