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Title: Multiscale spectral nanoscopy

A system and method for non-invasively tracking a particle in a sample is disclosed. The system includes a 2-photon or confocal laser scanning microscope (LSM) and a particle-holding device coupled to a stage with X-Y and Z position control. The system also includes a tracking module having a tracking excitation laser, X-Y and Z radiation-gathering components configured to detect deviations of the particle in an X-Y and Z directions. The system also includes a processor coupled to the X-Y and Z radiation gathering components, generate control signals configured to drive the stage X-Y and Z position controls to track the movement of the particle. The system may also include a synchronization module configured to generate LSM pixels stamped with stage position and a processing module configured to generate a 3D image showing the 3D trajectory of a particle using the LSM pixels stamped with stage position.
Inventors:
;
Issue Date:
OSTI Identifier:
1332117
Assignee:
THE TRUSTEES OF PRINCETON UNIVERSITY (Princeton, NJ) CHO
Patent Number(s):
9,494,518
Application Number:
14/415,779
Contract Number:
SC0006838
Resource Relation:
Patent File Date: 2013 Jul 18
Research Org:
THE TRUSTEES OF PRINCETON UNIVERSITY, Princeton, NJ (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 42 ENGINEERING; 59 BASIC BIOLOGICAL SCIENCES

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