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Title: Long-range barcode labeling-sequencing

Abstract

Methods for sequencing single large DNA molecules by clonal multiple displacement amplification using barcoded primers. Sequences are binned based on barcode sequences and sequenced using a microdroplet-based method for sequencing large polynucleotide templates to enable assembly of haplotype-resolved complex genomes and metagenomes.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1329319
Patent Number(s):
9,469,874
Application Number:
13/653,343
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Oct 16
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chen, Feng, Zhang, Tao, Singh, Kanwar K., Pennacchio, Len A., Froula, Jeff L., and Eng, Kevin S. Long-range barcode labeling-sequencing. United States: N. p., 2016. Web.
Chen, Feng, Zhang, Tao, Singh, Kanwar K., Pennacchio, Len A., Froula, Jeff L., & Eng, Kevin S. Long-range barcode labeling-sequencing. United States.
Chen, Feng, Zhang, Tao, Singh, Kanwar K., Pennacchio, Len A., Froula, Jeff L., and Eng, Kevin S. Tue . "Long-range barcode labeling-sequencing". United States. https://www.osti.gov/servlets/purl/1329319.
@article{osti_1329319,
title = {Long-range barcode labeling-sequencing},
author = {Chen, Feng and Zhang, Tao and Singh, Kanwar K. and Pennacchio, Len A. and Froula, Jeff L. and Eng, Kevin S.},
abstractNote = {Methods for sequencing single large DNA molecules by clonal multiple displacement amplification using barcoded primers. Sequences are binned based on barcode sequences and sequenced using a microdroplet-based method for sequencing large polynucleotide templates to enable assembly of haplotype-resolved complex genomes and metagenomes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {10}
}

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Works referenced in this record:

Comprehensive human genome amplification using multiple displacement amplification
journal, April 2002

  • Dean, F. B.; Hosono, S.; Fang, L.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 8, p. 5261-5266
  • DOI: 10.1073/pnas.082089499