Compositions and methods for making selenocysteine containing polypeptides

Soll, Dieter; Aldag, Caroline; Hohn, Michael

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Title: Compositions and methods for making selenocysteine containing polypeptides

Abstract

Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA.sup.Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA.sup.Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA.sup.Sec.

Inventors:: Soll, Dieter; Aldag, Caroline; Hohn, Michael

Issue Date:: Tue Oct 11 00:00:00 EDT 2016

Research Org.:: Yale Univ., New Haven, CT (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1328702

Patent Number(s):: 9464288

Application Number:: 14/131,382

Assignee:: Yale University (New Haven, CT)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K14/47 - from mammals

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/11 - DNA or RNA fragments
C12N15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N2310/531 - Stem-loop
C12N9/0004 - {Oxidoreductases
C12N9/0008 - {acting on the aldehyde or oxo group of donors
C12N9/10 - Transferases
C12N9/1007 - {Methyltransferases
C12N9/14 - Hydrolases
C12N9/93 - {Ligases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P21/00 - Preparation of peptides or proteins
C12P21/02 - having a known sequence of two or more amino acids, e.g. glutathione

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y209/01001 - L-Seryl-tRNA
C12Y306/05003 - Protein-synthesizing GTPase
C12Y601/01011 - Serine--tRNA ligase

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DOE Contract Number:: FG02-98ER20311

Resource Type:: Patent

Resource Relation:: Patent File Date: 2012 Jul 11

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

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                    Soll, Dieter, Aldag, Caroline, and Hohn, Michael. Compositions and methods for making selenocysteine containing polypeptides.  United States: N. p., 2016. 
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                    Soll, Dieter, Aldag, Caroline, & Hohn, Michael. Compositions and methods for making selenocysteine containing polypeptides.  United States.

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                    Soll, Dieter, Aldag, Caroline, and Hohn, Michael. Tue .  
        "Compositions and methods for making selenocysteine containing polypeptides".  United States.  https://www.osti.gov/servlets/purl/1328702.

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@article{osti_1328702,

  title        = {Compositions and methods for making selenocysteine containing polypeptides},

  author       = {Soll, Dieter and Aldag, Caroline and Hohn, Michael},

  abstractNote = {Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA.sup.Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA.sup.Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA.sup.Sec.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Oct 11 00:00:00 EDT 2016},

  month        = {Tue Oct 11 00:00:00 EDT 2016}

}

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Works referenced in this record:

Positional control of selenium insertion in polypeptides for X-ray crystallography
patent, December 1997

Leonard, Jack L.; Newburger, Peter
US Patent Document 5,700,660
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5700660

Selected polynucleotide and polypeptide sequences of the methanogenic archaeon, methanococcus jannashii
patent, January 2003

Bult, Carol J.; White, Owen; Smith, Hamilton O.
US Patent Document 6,503,729
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6503729

Site specific incorporation of phosphoserine into polypeptides using phosphoseryl-tRNA synthetase
patent, May 2010

Soll, Dieter; Rinehart, Jesse
US Patent Document 7,723,069
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7723069

Site-specific incorporation of phosphoserine into proteins in Escherichia coli
patent, July 2015

Park, Hee-Sung; Soll, Dieter
US Patent Document 9,090,928
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9090928

In vivo incorporation of unnatural amino acids
patent-application, May 2003

Schultz, Peter; Wang, Lei; Anderson, John Christopher
US Patent Application 10/126927; 20030082575
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030082575