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Title: Compositions and methods for making selenocysteine containing polypeptides

Abstract

Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA.sup.Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA.sup.Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA.sup.Sec.

Inventors:
; ;
Issue Date:
Research Org.:
Yale Univ., New Haven, CT (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1328702
Patent Number(s):
9,464,288
Application Number:
14/131,382
Assignee:
Yale University (New Haven, CT)
DOE Contract Number:  
FG02-98ER20311
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Jul 11
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Soll, Dieter, Aldag, Caroline, and Hohn, Michael. Compositions and methods for making selenocysteine containing polypeptides. United States: N. p., 2016. Web.
Soll, Dieter, Aldag, Caroline, & Hohn, Michael. Compositions and methods for making selenocysteine containing polypeptides. United States.
Soll, Dieter, Aldag, Caroline, and Hohn, Michael. Tue . "Compositions and methods for making selenocysteine containing polypeptides". United States. https://www.osti.gov/servlets/purl/1328702.
@article{osti_1328702,
title = {Compositions and methods for making selenocysteine containing polypeptides},
author = {Soll, Dieter and Aldag, Caroline and Hohn, Michael},
abstractNote = {Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA.sup.Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA.sup.Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA.sup.Sec.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {10}
}

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