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Title: Compositions and methods for making selenocysteine containing polypeptides

Abstract

Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA.sup.Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA.sup.Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA.sup.Sec.

Inventors:
; ;
Issue Date:
Research Org.:
Yale Univ., New Haven, CT (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1328702
Patent Number(s):
9464288
Application Number:
14/131,382
Assignee:
Yale University (New Haven, CT)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG02-98ER20311
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Jul 11
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Soll, Dieter, Aldag, Caroline, and Hohn, Michael. Compositions and methods for making selenocysteine containing polypeptides. United States: N. p., 2016. Web.
Soll, Dieter, Aldag, Caroline, & Hohn, Michael. Compositions and methods for making selenocysteine containing polypeptides. United States.
Soll, Dieter, Aldag, Caroline, and Hohn, Michael. Tue . "Compositions and methods for making selenocysteine containing polypeptides". United States. https://www.osti.gov/servlets/purl/1328702.
@article{osti_1328702,
title = {Compositions and methods for making selenocysteine containing polypeptides},
author = {Soll, Dieter and Aldag, Caroline and Hohn, Michael},
abstractNote = {Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA.sup.Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA.sup.Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA.sup.Sec.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {10}
}

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Works referenced in this record:

Crystal Structure of Formate Dehydrogenase H: Catalysis Involving Mo, Molybdopterin, Selenocysteine, and an Fe4S4 Cluster
journal, February 1997


Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea
journal, March 2007


An engineered selenocysteine defines a unique class of antibody derivatives
journal, August 2008


Five-base codons for incorporation of nonnatural amino acids into proteins
journal, September 2001


Sulfur and Selenium: The Role of Oxidation State in Protein Structure and Function
journal, October 2003


Selenocysteine in proteins—properties and biotechnological use
journal, October 2005


Characterization of Mammalian Selenoproteomes
journal, May 2003


Catalytic Mechanism of Sep-tRNA:Cys-tRNA Synthase: Sulfur Transfer Is Mediated By Disulfide And Persulfide
journal, December 2011


The Human SepSecS-tRNASec Complex Reveals the Mechanism of Selenocysteine Formation
journal, July 2009


The selenocysteine-inserting opal suppressor serine tRNA from E.coli is highly unusual in structure and modification
journal, January 1989


Identification and characterization of a selenoprotein family containing a diselenide bond in a redox motif
journal, August 2007


Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
journal, January 2008


Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O -phosphoseryl-tRNA Sec kinase
journal, February 2008


The antioxidant role of selenium and seleno-compounds
journal, May 2003


New Developments in Selenium Biochemistry: Selenocysteine Biosynthesis in Eukaryotes and Archaea
journal, August 2007


The many levels of control on bacterial selenoprotein synthesis
journal, November 2009


RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea
journal, December 2006


Basic local alignment search tool
journal, October 1990


Cys-tRNACys formation and cysteine biosynthesis in methanogenic archaea: two faces of the same problem?
journal, October 2004


Structurally Modified Firefly Luciferase. Effects of Amino Acid Substitution at Position 286
journal, November 1997

  • Arslan, Tuncer; Mamaev, Sergey V.; Mamaeva, Natalia V.
  • Journal of the American Chemical Society, Vol. 119, Issue 45, p. 10877-10887
  • https://doi.org/10.1021/ja971927a

Mutagenesis-based definitions and probes of residue burial in proteins
journal, October 2005


Phosphoserine Aminotransferase, the Second Step-Catalyzing Enzyme for Serine Biosynthesis
journal, November 1999


Photoreactive Bicyclic Amino Acids as Substrates for Mutant Escherichia coli Phenylalanyl-tRNA Synthetases
journal, March 2004


Positive-selection vectors using the F plasmid ccdB killer gene
journal, October 1994


The unusual methanogenic seryl‐tRNA synthetase recognizes tRNASer species from all three kingdoms of life
journal, January 2004


Direct charging of tRNACUA with pyrrolysine in vitro and in vivo
journal, August 2004


A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodies
journal, September 1992


A dual-specificity aminoacyl-tRNA synthetase in the deep-rooted eukaryote Giardia lamblia
journal, November 2000


The Catalytic Properties of Tyrosyl Ribonucleic Acid Synthetases from Escherichia coli and Bacillus subtilis
journal, May 1966


The Affinity of Elongation Factor Tu for an Aminoacyl-tRNA Is Modulated by the Esterified Amino Acid
journal, May 2004


One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000


[15] Biosynthetic method for introducing unnatural amino acids site-specifically into proteins
book, January 1991


The insect tracheal system: a conduit for the systemic spread of Autographa californica M nuclear polyhedrosis virus.
journal, April 1994


An aminoacyl tRNA synthetase whose sequence fits into neither of the two known classes
journal, May 2001


High-level secretion of correctly processed recombinant human interleukin-1β in Kluyveromyces lactis
journal, November 1991


A proteomic view of the Plasmodium falciparum life cycle
journal, October 2002


Global analysis of a “simple” proteome: Methanococcus jannaschii
journal, December 2002


Formation of de novo centromeres and construction of first-generation human artificial microchromosomes
journal, April 1997


Emergence of the universal genetic code imprinted in an RNA record
journal, November 2006


Toward understanding phosphoseryl-tRNACys formation: The crystal structure of Methanococcus maripaludis phosphoseryl-tRNA synthetase
journal, February 2007


High-velocity microprojectiles for delivering nucleic acids into living cells
journal, May 1987


Purification and characterization of IL6-PE4e, a recombinant fusion of interleukin 6 with Pseudomonas exotoxin
journal, November 1993


Uniform Binding of Aminoacyl-tRNAs to Elongation Factor Tu by Thermodynamic Compensation
journal, October 2001


Cysteinyl-tRNA formation: the last puzzle of aminoacyl-tRNA synthesis
journal, November 1999


Usage of an intronic promoter for stable gene expression in Saccharomyces cerevisiae
journal, May 2005


Pathogenic mechanism of a human mitochondrial tRNAPhe mutation associated with myoclonic epilepsy with ragged red fibers syndrome
journal, September 2007


Phenylalanyl-tRNA synthetase editing defects result in efficient mistranslation of phenylalanine codons as tyrosine
journal, September 2007


Synthesis of Cysteinyl-tRNACys by a Genome That Lacks the Normal Cysteine-tRNA Synthetase
journal, July 2000


Possible incorporation of phosphoserine into globin readthrough protein via bovine opal suppressor phosphoseryl-tRNA
journal, October 1986


Design and Evolution of New Catalytic Activity with an Existing Protein Scaffold
journal, January 2006


Expanding the Genetic Code of Escherichia coli with Phosphoserine
journal, August 2011


An aminoacyl-tRNA synthetase that specifically activates pyrrolysine
journal, August 2004


Gene Transfer into Hepatocytes and Human Liver Tissue by Baculovirus Vectors
journal, October 1996


Understanding the Sequence Specificity of tRNA Binding to Elongation Factor Tu using tRNA Mutagenesis
journal, March 2009


Cysteinyl-tRNA synthetase is not essential for viability of the archaeon Methanococcus maripaludis
journal, November 2001


One Polypeptide with Two Aminoacyl-tRNA Synthetase Activities
journal, January 2000


Protein production by auto-induction in high-density shaking cultures
journal, May 2005


DTASelect and Contrast:  Tools for Assembling and Comparing Protein Identifications from Shotgun Proteomics
journal, February 2002


The role of tightly bound ATP in Escherichia coli tRNA nucleotidyltransferase
journal, September 2000


Expanding the Genetic Code of Escherichia coli
journal, April 2001


Molecular genetic studies of a 10.9-kb operon in Escherichia coli for phosphonate uptake and biodegradation
journal, December 1992


The biosynthesis of cysteine and homocysteine in Methanococcus jannaschii
journal, December 2003


Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNACys
journal, April 2008