Intein-modified enzymes, their production and industrial applications

Apgar, James; Lessard, Philip; Raab, Michael R.; Shen, Binzhang; Lazar, Gabor; de la Vega, Humberto

Title: Intein-modified enzymes, their production and industrial applications

Abstract

A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-.beta.-sheet junction or a loop-.alpha.-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.

Inventors:: Apgar, James; Lessard, Philip; Raab, Michael R.; Shen, Binzhang; Lazar, Gabor; de la Vega, Humberto

Issue Date:: 2016-10-11

Research Org.:: Agrivida, Inc. Medford, MA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1328698

Patent Number(s):: 9464333

Application Number:: 13/508,234

Assignee:: Agrivida, Inc. (Medford, MA

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/248 - {Xylanases}
C12N15/8216 - {Methods for controlling, regulating or enhancing expression of transgenes in plant cells}
C12N9/2437 - {Cellulases

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y302/01008 - Endo-1,4-beta-xylanase

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/8246 - {Non-starch polysaccharides, e.g. cellulose, fructans, levans}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y302/01004 - Cellulase

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DOE Contract Number:: AR0000042

Resource Type:: Patent

Resource Relation:: Patent File Date: 2010 Nov 05

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Apgar, James, Lessard, Philip, Raab, Michael R., Shen, Binzhang, Lazar, Gabor, and de la Vega, Humberto. Intein-modified enzymes, their production and industrial applications.  United States: N. p., 2016. 
        Web.

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                    Apgar, James, Lessard, Philip, Raab, Michael R., Shen, Binzhang, Lazar, Gabor, & de la Vega, Humberto. Intein-modified enzymes, their production and industrial applications.  United States.

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                    Apgar, James, Lessard, Philip, Raab, Michael R., Shen, Binzhang, Lazar, Gabor, and de la Vega, Humberto. Tue .  
        "Intein-modified enzymes, their production and industrial applications".  United States.  https://www.osti.gov/servlets/purl/1328698.

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@article{osti_1328698,

  title        = {Intein-modified enzymes, their production and industrial applications},

  author       = {Apgar, James and Lessard, Philip and Raab, Michael R. and Shen, Binzhang and Lazar, Gabor and de la Vega, Humberto},

  abstractNote = {A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-.beta.-sheet junction or a loop-.alpha.-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2016},

  month        = {10}

}

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