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Title: Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification


Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.

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Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
OSTI Identifier:
Patent Number(s):
Application Number:
Lawrence Livermore National Security, LLC (Livermore, CA)
DOE Contract Number:  
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Patent File Date: 2014 Nov 07
Country of Publication:
United States

Citation Formats

Chromy, Brett A., Henderson, Paul, and Hoeprich, Jr, Paul D. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification. United States: N. p., 2016. Web.
Chromy, Brett A., Henderson, Paul, & Hoeprich, Jr, Paul D. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification. United States.
Chromy, Brett A., Henderson, Paul, and Hoeprich, Jr, Paul D. Tue . "Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification". United States.
title = {Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification},
author = {Chromy, Brett A. and Henderson, Paul and Hoeprich, Jr, Paul D.},
abstractNote = {Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {10}


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