skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Abstract

Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.

Inventors:
; ;
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1327989
Patent Number(s):
9,458,191
Application Number:
14/536,513
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Nov 07
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Chromy, Brett A., Henderson, Paul, and Hoeprich, Jr, Paul D. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification. United States: N. p., 2016. Web.
Chromy, Brett A., Henderson, Paul, & Hoeprich, Jr, Paul D. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification. United States.
Chromy, Brett A., Henderson, Paul, and Hoeprich, Jr, Paul D. Tue . "Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification". United States. https://www.osti.gov/servlets/purl/1327989.
@article{osti_1327989,
title = {Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification},
author = {Chromy, Brett A. and Henderson, Paul and Hoeprich, Jr, Paul D.},
abstractNote = {Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {10}
}

Patent:

Save / Share:

Works referenced in this record:

Overview of Protein Structural and Functional Folds
book, February 2004

  • Sun, Peter D.; Foster, Christine E.; Boyington, Jeffrey C.
  • Current Protocols in Protein Science, Vol. 35, p. 17.1.1.1–17.1.189
  • DOI: 10.1002/0471140864.ps1701s35

Predictable difficulty or difficulty to predict: Letter to the Editor
journal, December 2010

  • Arányi, Tamás; Fülöp, Krisztina; Symmons, Orsolya
  • Protein Science, Vol. 20, Issue 1
  • DOI: 10.1002/pro.552

Synthesis and Characterization of Chelator-Lipids for Reversible Immobilization of Engineered Proteins at Self-Assembled Lipid Interfaces
journal, September 1994

  • Schmitt, Lutz; Dietrich, Christian; Tampe, Robert
  • Journal of the American Chemical Society, Vol. 116, Issue 19, p. 8485-8491
  • DOI: 10.1021/ja00098a008

Detection of DNA hybridization on indium tin oxide surfaces
journal, August 2007

  • Moses, Selina; Brewer, Scott H.; Kraemer, Stephan
  • Sensors and Actuators B: Chemical, Vol. 125, Issue 2, p. 574-580
  • DOI: 10.1016/j.snb.2007.03.009

High-Sensitivity Detection of DNA Hybridization on Microarrays Using Resonance Light Scattering
journal, April 2002

  • Bao, Paul; Frutos, Anthony G.; Greef, Charles
  • Analytical Chemistry, Vol. 74, Issue 8
  • DOI: 10.1021/ac0111964

Exploring the new world of the genome with DNA microarrays
journal, January 1999

  • Brown, Patrick O.; Botstein, David
  • Nature Genetics, Vol. 21, Issue S1
  • DOI: 10.1038/4462

Formation of Thiolate and Phosphonate Adlayers on Indium−Tin Oxide:  Optical and Electronic Characterization
journal, September 2002

  • Brewer, Scott H.; Brown, Derek A.; Franzen, Stefan
  • Langmuir, Vol. 18, Issue 18
  • DOI: 10.1021/la015720d

Progress towards the easier use of P450 enzymes
journal, January 2006

  • Chefson, Amandine; Auclair, Karine
  • Molecular BioSystems, Vol. 2, Issue 10
  • DOI: 10.1039/B607001A

Some Observations on the Type I and Type II Microsomal Binding Spectra
journal, January 1971


Amino acids in SRS1 and SRS6 are critical for furanocoumarin metabolism by CYP6B1v1, a cytochrome P450 monooxygenase
journal, April 2002


How Translocons Select Transmembrane Helices
journal, June 2008


Construction and characterization of a second-generation pseudoinfectious West Nile virus vaccine propagated using a new cultivation system
journal, May 2008


Immobilization of His-Tagged Proteins on Nickel-Chelating Nanolipoprotein Particles
journal, March 2009

  • Fischer, Nicholas O.; Blanchette, Craig D.; Chromy, Brett A.
  • Bioconjugate Chemistry, Vol. 20, Issue 3
  • DOI: 10.1021/bc8003155

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles
journal, February 2010

  • Sperling, R. A.; Parak, W. J.
  • Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 368, Issue 1915, p. 1333-1383
  • DOI: 10.1098/rsta.2009.0273

Three-Dimensional Graphene Nano-Networks with High Quality and Mass Production Capability via Precursor-Assisted Chemical Vapor Deposition
journal, May 2013

  • Yoon, Jong-Chul; Lee, Jung-Soo; Kim, Sun-I
  • Scientific Reports, Vol. 3, Issue 1
  • DOI: 10.1038/srep01788

A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein
journal, April 2007

  • Whorton, M. R.; Bokoch, M. P.; Rasmussen, S. G. F.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 18, p. 7682-7687
  • DOI: 10.1073/pnas.0611448104

Synthesis of Native Proteins by Chemical Ligation
journal, June 2000


Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems
journal, January 2003


The growing impact of click chemistry on drug discovery
journal, December 2003


Large-scale profiling of protein palmitoylation in mammalian cells
journal, January 2009

  • Martin, Brent R.; Cravatt, Benjamin F.
  • Nature Methods, Vol. 6, Issue 2, p. 135-138
  • DOI: 10.1038/nmeth.1293

Systems for orthogonal self-assembly of electroactive monolayers on Au and ITO: an approach to molecular electronics
journal, July 1995

  • Gardner, Timothy J.; Frisbie, C. Daniel; Wrighton, Mark S.
  • Journal of the American Chemical Society, Vol. 117, Issue 26, p. 6927-6933
  • DOI: 10.1021/ja00131a015

The Efficiency of Light-Directed Synthesis of DNA Arrays on Glass Substrates
journal, June 1997

  • McGall, Glenn H.; Barone, Anthony D.; Diggelmann, Martin
  • Journal of the American Chemical Society, Vol. 119, Issue 22, p. 5081-5090
  • DOI: 10.1021/ja964427a

Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array
journal, October 1999

  • Singh-Gasson, Sangeet; Green, Roland D.; Yue, Yongjian
  • Nature Biotechnology, Vol. 17, Issue 10, p. 974-978
  • DOI: 10.1038/13664

CpG DNA induces stronger immune responses with less toxicity than other adjuvants
journal, March 2000


Induction of tumor necrosis factor-α in solid tumor region by the orally administered synthetic muramyl dipeptide analogue, romurtide
journal, January 2001


Identification of active methylotroph populations in an acidic forest soil by stable-isotope probing c
journal, August 2002


RNA Stable Isotope Probing, a Novel Means of Linking Microbial Community Function to Phylogeny
journal, November 2002


DNA-based stable isotope probing: a link between community structure and function
journal, June 2009

  • Uhlík, Ondrej; Jecná, Katerina; Leigh, Mary Beth
  • Science of The Total Environment, Vol. 407, Issue 12, p. 3611-3619
  • DOI: 10.1016/j.scitotenv.2008.05.012

Stable isotope probing: Technical considerations when resolving 15N-labeled RNA in gradients
journal, January 2010

  • Addison, S. L.; McDonald, I. R.; Lloyd-Jones, G.
  • Journal of Microbiological Methods, Vol. 80, Issue 1, p. 70-75
  • DOI: 10.1016/j.mimet.2009.11.002

The contribution of macrophyte-derived organic matter to microbial biomass in salt-marsh sediments: Stable carbon isotope analysis of microbial biomarkers
journal, March 1999

  • Boschker, H. T. S.; de Brouwer, J. F. C.; Cappenberg, T. E.
  • Limnology and Oceanography, Vol. 44, Issue 2
  • DOI: 10.4319/lo.1999.44.2.0309

The Isotope Array, a New Tool That Employs Substrate-Mediated Labeling of rRNA for Determination of Microbial Community Structure and Function
journal, November 2003


Integration of biological networks and gene expression data using Cytoscape
journal, September 2007

  • Cline, Melissa S.; Smoot, Michael; Cerami, Ethan
  • Nature Protocols, Vol. 2, Issue 10
  • DOI: 10.1038/nprot.2007.324

Specific targeting of a lipophilic prodrug of iododeoxyuridine to parenchymal liver cells using lactosylated reconstituted high density lipoprotein particles
journal, July 1996

  • Bijsterbosch, Martin K.; van de Bilt, Hendrika; van Berkel, Theo J. C.
  • Biochemical Pharmacology, Vol. 52, Issue 1, p. 113-121
  • DOI: 10.1016/0006-2952(96)00170-0

Indium-111 Labeling of Low Density Lipoproteins with the DTPA−Bis(stearylamide):  Evaluation as a Potential Radiopharmaceutical for Tumor Localization
journal, January 1996

  • Jasanada, François; Urizzi, Pascale; Souchard, Jean-Pierre
  • Bioconjugate Chemistry, Vol. 7, Issue 1
  • DOI: 10.1021/bc950073l

In vitro characterization of binding and stability of single-chain Fv Ni-NTA-liposomes
journal, January 2006


Preparation and Characterization of Nickel Nanoparticles for Binding to His-tag Proteins and Antigens
journal, December 2006


Protein Translocons
journal, February 2003


Homotropic cooperativity of monomeric cytochrome P450 3A4 in a nanoscale native bilayer environment
journal, October 2004

  • Baas, Bradley J.; Denisov, Ilia G.; Sligar, Stephen G.
  • Archives of Biochemistry and Biophysics, Vol. 430, Issue 2, p. 218-228
  • DOI: 10.1016/j.abb.2004.07.003

Hydrogen Production by a Hyperthermophilic Membrane-Bound Hydrogenase in Water-Soluble Nanolipoprotein Particles
journal, June 2009

  • Baker, Sarah E.; Hopkins, Robert C.; Blanchette, Craig D.
  • Journal of the American Chemical Society, Vol. 131, Issue 22
  • DOI: 10.1021/ja809251f

Single-molecule height measurements on microsomal cytochrome P450 in nanometer-scale phospholipid bilayer disks
journal, May 2002

  • Bayburt, T. H.; Sligar, S. G.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 10, p. 6725-6730
  • DOI: 10.1073/pnas.062565599

Transducin Activation by Nanoscale Lipid Bilayers Containing One and Two Rhodopsins
journal, March 2007

  • Bayburt, Timothy H.; Leitz, Andrew J.; Xie, Guifu
  • Journal of Biological Chemistry, Vol. 282, Issue 20
  • DOI: 10.1074/jbc.M701433200

Direct linking of microbial populations to specific biogeochemical processes by 13C-labelling of biomarkers
journal, April 1998

  • Boschker, H. T. S.; Nold, S. C.; Wellsbury, P.
  • Nature, Vol. 392, Issue 6678
  • DOI: 10.1038/33900

Urban aerosols harbor diverse and dynamic bacterial populations
journal, December 2006

  • Brodie, E. L.; DeSantis, T. Z.; Parker, J. P. M.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 1, p. 299-304
  • DOI: 10.1073/pnas.0608255104

Cell-free Co-expression of Functional Membrane Proteins and Apolipoprotein, Forming Soluble Nanolipoprotein Particles
journal, July 2008

  • Cappuccio, Jenny A.; Blanchette, Craig D.; Sulchek, Todd A.
  • Molecular & Cellular Proteomics, Vol. 7, Issue 11
  • DOI: 10.1074/mcp.M800191-MCP200

Stable-isotope probing as a tool in microbial ecology
journal, February 2000

  • Radajewski, Stefan; Ineson, Philip; Parekh, Nisha R.
  • Nature, Vol. 403, Issue 6770, p. 646-649
  • DOI: 10.1038/35001054

Evaluation of Nanolipoprotein Particles (NLPs) as an In Vivo Delivery Platform
journal, March 2014


Kinetic properties of recombinant MAO-A on incorporation into phospholipid nanodisks
journal, March 2007


Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
journal, July 2006

  • DeSantis, T. Z.; Hugenholtz, P.; Larsen, N.
  • Applied and Environmental Microbiology, Vol. 72, Issue 7, p. 5069-5072
  • DOI: 10.1128/AEM.03006-05

High-Density Universal 16S rRNA Microarray Analysis Reveals Broader Diversity than Typical Clone Library When Sampling the Environment
journal, March 2007


An improved synthesis of isopentenyl pyrophosphate
journal, November 1967

  • Donninger, C.; Popják, G.
  • Biochemical Journal, Vol. 105, Issue 2
  • DOI: 10.1042/bj1050545

Enzyme-catalyzed Synthesis and Degradation of Biopolymers
journal, February 2009


Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors
journal, May 2005

  • Ishihara, Goshi; Goto, Mie; Saeki, Mihoro
  • Protein Expression and Purification, Vol. 41, Issue 1, p. 27-37
  • DOI: 10.1016/j.pep.2005.01.013

Insertion of Membrane Proteins into Discoidal Membranes Using a Cell-Free Protein Expression Approach
journal, August 2008

  • Katzen, Federico; Fletcher, Julia E.; Yang, Jian-Ping
  • Journal of Proteome Research, Vol. 7, Issue 8
  • DOI: 10.1021/pr800265f

Biodegradation of Natural Rubber and Related Compounds: Recent Insights into a Hardly Understood Catabolic Capability of Microorganisms
journal, June 2005


The Biosynthesis of Natural Rubber
journal, June 1990

  • Paterson-Jones, J. C.; Gilliland, M. G.; Van Staden, J.
  • Journal of Plant Physiology, Vol. 136, Issue 3, p. 257-263
  • DOI: 10.1016/S0176-1617(11)80047-7

Model of Biologically Active Apolipoprotein E Bound to Dipalmitoylphosphatidylcholine
journal, November 2005

  • Peters-Libeu, Clare A.; Newhouse, Yvonne; Hatters, Danny M.
  • Journal of Biological Chemistry, Vol. 281, Issue 2
  • DOI: 10.1074/jbc.M510851200

Transcriptome and gene expression analysis in cold-acclimated guayule (Parthenium argentatum) rubber-producing tissue
journal, July 2012


Recombinant lipoproteins: lipoprotein-like lipid particles for drug targeting
journal, April 2001

  • Rensen, Patrick C. N.; de Vrueh, Remco L. A.; Kuiper, Johan
  • Advanced Drug Delivery Reviews, Vol. 47, Issue 2-3, p. 251-276
  • DOI: 10.1016/S0169-409X(01)00109-0

A bilayer cell-free protein synthesis system for high-throughput screening of gene products
journal, February 2002


Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz
journal, January 2010

  • Schmidt, Thomas; Lenders, Malte; Hillebrand, Andrea
  • BMC Biochemistry, Vol. 11, Issue 1
  • DOI: 10.1186/1471-2091-11-11

Phospholipid phase transitions in homogeneous nanometer scale bilayer discs
journal, December 2003


The micromorphology and protein characterization of rubber particles in Ficus carica, Ficus benghalensis and Hevea brasiliensis
journal, March 2003

  • Singh, A. P.; Wi, S. G.; Chung, G. C.
  • Journal of Experimental Botany, Vol. 54, Issue 384
  • DOI: 10.1093/jxb/erg107

Indirect electrochemical reduction of nicotinamide coenzymes
journal, December 2004


Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms
journal, April 1998


Initiation of rubber biosynthesis: In vitro comparisons of benzophenone-modified diphosphate analogues in three rubber-producing species
journal, October 2008


Gold Nanoparticle-Enhanced Secondary Ion Mass Spectrometry Imaging of Peptides on Self-Assembled Monolayers
journal, March 2006

  • Kim, Young-Pil; Oh, Eunkeu; Hong, Mi-Young
  • Analytical Chemistry, Vol. 78, Issue 6
  • DOI: 10.1021/ac051500j

Protein tolerance to random amino acid change
journal, June 2004

  • Guo, H. H.; Choe, J.; Loeb, L. A.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210
  • DOI: 10.1073/pnas.0403255101

Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities.
journal, March 1988

  • Lazar, E.; Watanabe, S.; Dalton, S.
  • Molecular and Cellular Biology, Vol. 8, Issue 3, p. 1247-1252
  • DOI: 10.1128/MCB.8.3.1247

Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia coli
journal, March 1998

  • Hill, Margaret A.; Preiss, Jack
  • Biochemical and Biophysical Research Communications, Vol. 244, Issue 2, p. 573-577
  • DOI: 10.1006/bbrc.1998.8301

Disentangling the perturbational effects of amino acid substitutions in the DNA-binding domain of p53
journal, February 1999

  • Wacey, A. I.; Cooper, D. N.; Liney, D.
  • Human Genetics, Vol. 104, Issue 1, p. 15-22
  • DOI: 10.1007/s004390050904

Rubber Elongation Factor (REF), a Major Allergen Component in Hevea brasiliensis Latex Has Amyloid Properties
journal, October 2012


Protein engineering methods applied to membrane protein targets
journal, October 2012

  • Lluis, M. W.; Godfroy, J. I.; Yin, H.
  • Protein Engineering Design and Selection, Vol. 26, Issue 2
  • DOI: 10.1093/protein/gzs079

The Soluble NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha H16 Consists of Six Subunits and Can Be Specifically Activated by NADPH
journal, April 2005


Hydrogen production by biological processes: a survey of literature
journal, January 2001


Co-incorporation of heterologously expressed Arabidopsis cytochrome P450 and P450 reductase into soluble nanoscale lipid bilayers
journal, April 2004

  • Duan, Hui; Civjan, Natanya R; Sligar, Stephen G
  • Archives of Biochemistry and Biophysics, Vol. 424, Issue 2, p. 141-153
  • DOI: 10.1016/j.abb.2004.02.010

Biological hydrogen production; fundamentals and limiting processes
journal, November 2002


Structure and function of cytochromes P450:a comparative analysis of three crystal structures
journal, January 1995


Bio-hydrogen production from waste materials
journal, March 2006


Characterization of a HoxEFUYH type of [NiFe] hydrogenase from Allochromatium vinosum and some EPR and IR properties of the hydrogenase module
journal, September 2006

  • Long, Minnan; Liu, Jingjing; Chen, Zhifeng
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 12, Issue 1
  • DOI: 10.1007/s00775-006-0162-1

The [NiFe]-Hydrogenase of the Cyanobacterium Synechocystis sp. PCC 6803 Works Bidirectionally with a Bias to H 2 Production
journal, July 2011

  • McIntosh, Chelsea L.; Germer, Frauke; Schulz, Rüdiger
  • Journal of the American Chemical Society, Vol. 133, Issue 29
  • DOI: 10.1021/ja203376y

Intact Functional Fourteen-subunit Respiratory Membrane-bound [NiFe]-Hydrogenase Complex of the Hyperthermophilic Archaeon Pyrococcus furiosus
journal, May 2014

  • McTernan, Patrick M.; Chandrayan, Sanjeev K.; Wu, Chang-Hao
  • Journal of Biological Chemistry, Vol. 289, Issue 28
  • DOI: 10.1074/jbc.M114.567255

Purification and catalytic properties of Ech hydrogenase from Methanosarcina barkeri
journal, October 1999


Cyanobacterial-Type, Heteropentameric, NAD+-Reducing NiFe Hydrogenase in the Purple Sulfur Photosynthetic Bacterium Thiocapsa roseopersicina
journal, February 2004


HoxE—a subunit specific for the pentameric bidirectional hydrogenase complex (HoxEFUYH) of cyanobacteria
journal, April 2002

  • Schmitz, Oliver; Boison, Gudrun; Salzmann, Heike
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1554, Issue 1-2, p. 66-74
  • DOI: 10.1016/S0005-2728(02)00214-1

Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans: CO-oxidizing:H2-evolving enzyme complex
journal, October 2002


Modulation of human erg K+ channel gating by activation of a G protein-coupled receptor and protein kinase C
journal, September 1998


Dopamine tone regulates D1 receptor trafficking and delivery in striatal neurons in dopamine transporter-deficient mice
journal, February 2000

  • Dumartin, B.; Jaber, M.; Gonon, F.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 4, p. 1879-1884
  • DOI: 10.1073/pnas.97.4.1879

Corticotropin Releasing Factor (CRF) Receptor Signaling in the Central Nervous System: New Molecular Targets
journal, August 2006

  • Hauger, Richard; Risbrough, Victoria; Brauns, Olaf
  • CNS & Neurological Disorders - Drug Targets, Vol. 5, Issue 4
  • DOI: 10.2174/187152706777950684

G-Protein-Coupled Receptor Microarrays for Multiplexed Compound Screening
journal, June 2006

  • Hong, Yulong; Webb, Brian L.; Pai, Sadashiva
  • Journal of Biomolecular Screening, Vol. 11, Issue 4
  • DOI: 10.1177/1087057106287139

The Effects of Deleting the Mouse Neurotensin Receptor NTR1 on Central and Peripheral Responses to Neurotensin
journal, January 2002

  • Pettibone, Douglas J.; Hess, J. Fred; Hey, Patricia J.
  • Journal of Pharmacology and Experimental Therapeutics, Vol. 300, Issue 1
  • DOI: 10.1124/jpet.300.1.305

Different G protein-coupled receptor kinases govern G protein and β-arrestin-mediated signaling of V2 vasopressin receptor
journal, January 2005

  • Ren, X.-R.; Reiter, E.; Ahn, S.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 5, p. 1448-1453
  • DOI: 10.1073/pnas.0409534102

Molecular Dynamics Simulations of Discoidal Bilayers Assembled from Truncated Human Lipoproteins
journal, January 2005


Novel applications of liposomes
journal, July 1998


Osmotic shrinkage of giant egg-lecithin vesicles
journal, April 1981


Surface changes induced by osmotic shrinkage on large unilamellar vesicles
journal, November 1996


Stability of pressure-extruded liposomes made from archaeobacterial ether lipids
journal, November 1994

  • Choquet, C. G.; Patel, G. B.; Sprott, G. D.
  • Applied Microbiology and Biotechnology, Vol. 42, Issue 2-3, p. 375-384
  • DOI: 10.1007/BF00902745

Mechanical properties and stability measurement of cholesterol-containing liposome on mica by atomic force microscopy
journal, October 2004

  • Liang, Xuemei; Mao, Guangzhao; Ng, K. Y. Simon
  • Journal of Colloid and Interface Science, Vol. 278, Issue 1, p. 53-62
  • DOI: 10.1016/j.jcis.2004.05.042

Steric stabilization of phospholipid vesicles by block copolymers Vesicle flocculation and osmotic swelling caused by monovalent and divalent cations
journal, January 1998

  • Kostarelos, Kostas; Luckham, Paul F.; Tadros, Tharwat F.
  • Journal of the Chemical Society, Faraday Transactions, Vol. 94, Issue 15
  • DOI: 10.1039/A801134F

Adjuvants for human vaccines—current status, problems and future prospects
journal, January 1995


The Vaccine Adjuvant Monophosphoryl Lipid A as a TRIF-Biased Agonist of TLR4
journal, June 2007


Taking toll: lipid A mimetics as adjuvants and immunomodulators
journal, October 2002


Generation of immunoliposomes using recombinant single-chain Fv fragments bound to Ni-NTA-liposomes
journal, August 2005


Attaching histidine-tagged peptides and proteins to lipid-based carriers through use of metal-ion-chelating lipids
journal, December 2002

  • Chikh, Ghania G.; Li, Wai Ming; Schutze-Redelmeier, Marie-Paule
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1567, p. 204-212
  • DOI: 10.1016/S0005-2736(02)00618-1

Vaccine manufacturing: challenges and solutions
journal, November 2006

  • Ulmer, Jeffrey B.; Valley, Ulrich; Rappuoli, Rino
  • Nature Biotechnology, Vol. 24, Issue 11
  • DOI: 10.1038/nbt1261

Characterization and Purification of Polydisperse Reconstituted Lipoproteins and Nanolipoprotein Particles
journal, July 2009

  • Blanchette, Craig; Segelke, Brent; Fischer, Nicholas
  • International Journal of Molecular Sciences, Vol. 10, Issue 7
  • DOI: 10.3390/ijms10072958

Conjugation to Nickel-Chelating Nanolipoprotein Particles Increases the Potency and Efficacy of Subunit Vaccines to Prevent West Nile Encephalitis
journal, June 2010

  • Fischer, Nicholas O.; Infante, Ernesto; Ishikawa, Tomohiro
  • Bioconjugate Chemistry, Vol. 21, Issue 6, p. 1018-1022
  • DOI: 10.1021/bc100083d

Two-Dimensional Streptavidin Crystals on Giant Lipid Bilayer Vesicles
journal, May 2002

  • Ratanabanangkoon, Pasut; Gropper, Michael; Merkel, Rudolf
  • Langmuir, Vol. 18, Issue 11, p. 4270-4276
  • DOI: 10.1021/la025568v

Specific Interaction and Two-Dimensional Crystallization of Histidine Tagged Yeast RNA Polymerase I on Nickel-Chelating Lipids
journal, March 1998

  • Bischler, Nicolas; Balavoine, Fabrice; Milkereit, Philipp
  • Biophysical Journal, Vol. 74, Issue 3, p. 1522-1532
  • DOI: 10.1016/S0006-3495(98)77864-6

Two-Dimensional Crystallization of Histidine-Tagged, HIV-1 Reverse Transcriptase Promoted by a Novel Nickel-Chelating Lipid
journal, September 1994

  • Kubalek, Elizabeth W.; Le Grice, Stuart F. J.; Brown, Patrick O.
  • Journal of Structural Biology, Vol. 113, Issue 2
  • DOI: 10.1006/jsbi.1994.1039