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Title: Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Abstract

Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.

Inventors:
; ;
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1327989
Patent Number(s):
9458191
Application Number:
14/536,513
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Nov 07
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Chromy, Brett A., Henderson, Paul, and Hoeprich, Jr, Paul D.. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification. United States: N. p., 2016. Web.
Chromy, Brett A., Henderson, Paul, & Hoeprich, Jr, Paul D.. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification. United States.
Chromy, Brett A., Henderson, Paul, and Hoeprich, Jr, Paul D.. Tue . "Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification". United States. https://www.osti.gov/servlets/purl/1327989.
@article{osti_1327989,
title = {Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification},
author = {Chromy, Brett A. and Henderson, Paul and Hoeprich, Jr, Paul D.},
abstractNote = {Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {10}
}

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