Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification
Abstract
Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1327928
- Patent Number(s):
- 9458191
- Application Number:
- 14/536,513
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- DOE Contract Number:
- AC52-07NA27344
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2014 Nov 07
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Chromy, Brett A., Henderson, Paul, and Hoeprich, Jr, Paul D. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification. United States: N. p., 2016.
Web.
Chromy, Brett A., Henderson, Paul, & Hoeprich, Jr, Paul D. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification. United States.
Chromy, Brett A., Henderson, Paul, and Hoeprich, Jr, Paul D. Tue .
"Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification". United States. https://www.osti.gov/servlets/purl/1327928.
@article{osti_1327928,
title = {Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification},
author = {Chromy, Brett A. and Henderson, Paul and Hoeprich, Jr, Paul D.},
abstractNote = {Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {10}
}
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