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Title: Method for producing 3-hydroxypropionic acid and other products


This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.

; ;
Issue Date:
Research Org.:
Cargill, Incorporated, Wayzata, MN (United States)
Sponsoring Org.:
OSTI Identifier:
Patent Number(s):
Application Number:
Cargill, Incorporated (Wayzata, MN)
Patent Classifications (CPCs):
DOE Contract Number:  
Resource Type:
Resource Relation:
Patent File Date: 2014 Dec 18
Country of Publication:
United States

Citation Formats

Lynch, Michael D., Gill, Ryan T., and Lipscomb, Tanya E.W. Method for producing 3-hydroxypropionic acid and other products. United States: N. p., 2016. Web.
Lynch, Michael D., Gill, Ryan T., & Lipscomb, Tanya E.W. Method for producing 3-hydroxypropionic acid and other products. United States.
Lynch, Michael D., Gill, Ryan T., and Lipscomb, Tanya E.W. Tue . "Method for producing 3-hydroxypropionic acid and other products". United States.
title = {Method for producing 3-hydroxypropionic acid and other products},
author = {Lynch, Michael D. and Gill, Ryan T. and Lipscomb, Tanya E.W.},
abstractNote = {This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {8}

Works referenced in this record:

Production of Organic Compounds
patent, October 1946

Preparation of Esters of Acrylic Acid
patent, March 1949

Preparation of Acrylic Acid
patent, May 1949

Carboxylic Polymers
patent, July 1957

Polyacrylic acid having high chelation value and its production
patent, September 1975

Polymers for use in water treatment
patent, June 1977

Acrylic acid-acrylate copolymer thickening agents
patent, May 1981

Manufacture of polymers of acrylic acid or methacrylic acid
patent, November 1981

Absorbent article
patent, May 1987

Disposable diaper having density and basis weight profiled absorbent core
patent, August 1987

Suspending agent for the suspension polymerization of water-soluble monomers
patent, November 1987

Water absorbing agent
patent, March 1988

Process for preparing water-absorbing resins
patent, January 1991

Thin, flexible sanitary napkin
patent, April 1991

Super-absorbent polymer having improved absorbency properties
patent, September 1992

Process for production of water-absorbent resin
patent, January 1993

Continuous process for manufacture of a purified lactide
patent, December 1993

Hydrophilic, highly swellable hydrogels
patent, July 1994

Process for recycling aqueous fluid absorbents fines to a polymerizer
patent, August 1994

Catalyzed esterification process
patent, June 1995

Free radical copper(II)-enolate polymerization initiators
patent, November 1995

Free radical initiator delivery system
patent, April 1996

Lactic acid production, separation and/or recovery process
patent, April 1996

Process for the production of malonic acid or a salt thereof
patent, October 1998

Mutant phosphoenolpyruvate carboxylase, its gene, and production method of amino acid
patent, March 1999

Method for producing L-lysine
patent, December 1999

Method for the production of 1,3-propanediol by recombinant microorganisms
patent, January 2000

Microbial production of 1,2-propanediol from sugar
patent, July 2000

Method for producing nucleic acid substances
patent, September 2001

Water-absorbing agent and production process therefor
patent, October 2001

Method for purifying lactic acid
patent, December 2002

Lactic acid processing; methods; arrangements; and, products
patent, March 2003

Acid-dyeable polymer compositions
patent, April 2004

Production of 3-hydroxypropionic acid in recombinant organisms
patent, February 2005

Methods of making amino acids using E. coli transformed with csc genes
patent, November 2005

Method for producing target substance by fermentation
patent, August 2006

Single-stage separation and esterification of cation salt carboxylates using electrodeionization
patent, November 2006

Methods of making intermediates from polyhydroxyalkanoates
patent, January 2007

3-hydroxypropionic acid and other organic compounds
patent, March 2007

Process for preparing carboxylic acids and derivatives thereof
patent, March 2007

Mutant E. coli strain with increased succinic acid production
patent, May 2007

Process for separating and recovering 3-hydroxypropionic acid and acrylic acid
patent, October 2007

Alanine 2,3-Aminomutase
patent, December 2007

Increasing intracellular NADPH availability in E. coli
patent, February 2008

Production of 3-hydroxycarboxylic acid using nitrilase mutants
patent, April 2008

3-Hydroxypropionic acid and other organic compounds
patent, July 2008

Utilization of fructose in microbial production strains
patent, April 2009

Preparation of acrylic acid derivatives from α- or β-hydroxy carboxylic acids
patent, May 2009

3-hydroxypropionic acid and other organic compounds
patent, December 2009

Atom or group transfer radical polymerization
patent, March 2010

Method for conversion of β-hydroxy carbonyl compounds
patent, March 2010

Method for redesign of microbial production systems
patent, November 2010

Amino acid producing microorganism and a method for producing an amino acid
patent, November 2010

Method for producing an organic acid
patent, December 2011

Methods for producing 3-hydroxypropionic acid and other products
patent, November 2014

Production of Polyhydroxyalkanoates From Polyols
patent-application, November 2002

Schizochytrium PKS Genes
patent-application, May 2003

Novel Antimicrobial Activity of Gemfibrozil and Related Compounds and Derivatives and Metabolites Thereof
patent-application, October 2003

Bioabsorbable Polymer Containing 2-hydroxyacid Monomers
patent-application, November 2003

Expression of microbial proteins in plants for production of plants with improved properties
patent-application, December 2003

Production Of Polyketides
patent-application, December 2003

Method For Determining Gene Knockouts
patent-application, January 2004

3-hydroxypropionic Acid And Other Organic Compounds
patent-application, April 2004

Materials and methods for the efficient production of acetate and other products
patent-application, August 2004

Process for the Biological Production of 1,3-propanediol With High Yield
patent-application, August 2004

Synthesis of 1,2,3,4-tetrahydroxybenzenes From Biomass-derived Carbon
patent-application, October 2004

Process for Preparing Carboxylic Acids and Derivatives Thereof
patent-application, October 2004

Method for the Preparation of an Evolved Microorganism for the Creation or the Modification of Metabolic Pathways
patent-application, March 2005

Novel Enoyl Reductases and Methods of Use Thereof
patent-application, September 2005

Alanine 2,3,aminomutase
patent-application, October 2005

Preparation of acrylic acid derivatives from alpha-or beta-hydroxy carboxylic acids
patent-application, October 2005

Production Of Fermentation Product
patent-application, October 2005

Polyhydroxyalkanoate Production From Polyols
patent-application, October 2005

Process For Production And Purification Of Fermentation Derived Organic Acids
patent-application, December 2005

Process for Preparation of 1,3-propanediol
patent-application, December 2005

Process for Production of Organic Acid Esters
patent-application, January 2006

Instruments And Methods For Accessing An Anatomic Space
patent-application, January 2007

Optimised Micro-organism Strains For Nadph-consuming Biosynthetic Pathways
patent-application, April 2007

Production of 3-hydropropionic Acid Using Beta-alanine/pyruvate Aminotransferase
patent-application, May 2007

Process For Producing 1,3-propanediol and/or 3-hydroxypropionic Acid
patent-application, June 2007

3-hydroxypropionic acid and other organic compounds
patent-application, August 2007

Method and Apparatus for Conversion of Beta-hydroxy Carbonyl Compounds
patent-application, September 2007

Polyunsaturated fatty acid production in heterologous organisms using PUFA polyketide synthase systems
patent-application, October 2007

3-Hydroxypropionic Acid And Other Organic Compounds
patent-application, March 2008

Alanine 2, 3-aminomutase
patent-application, May 2008

Energy Efficient Methods To Produce Products
patent-application, August 2008

Methods and Organisms for Growth-Coupled Production of 3-Hydroxypropionic Acid
patent-application, August 2008

Membrane Supported Bioreactor For Conversion Of Syngas Components To Liquid Products
patent-application, January 2009

Water-absorbent polymer structures based on renewable resources and method for producing said structures
patent-application, January 2009

Alanine 2, 3 Aminomutases
patent-application, January 2009

3-hydroxypropionic acid and other organic compounds
patent-application, February 2009

Process for Converting a Hydroxycarboxylic Acid, or Salts Thereof, to an Unsaturated Carboxylic Acid and/or Its Esters
patent-application, March 2009

Method for the Production of Resveratrol in a Recombinant Oleaginous Microorganism
patent-application, March 2009

Production of Polyketides
patent-application, April 2009

Strain for butanol production
patent-application, August 2009

Methods and Composition for Extraction and Transesterification of Biomass Components
patent-application, September 2009

Beta-alanine/alpha-ketoglutarate Aminotransferase for 3-hydroxypropionic Acid Production
patent-application, November 2009

Methods of manufacturing derivatives of beta-hydroxycarboxylic acids
patent-application, December 2009

Deletion mutants for the production of isobutanol
patent-application, December 2009

Microbiological Production Of 3-Hydroxypropionic Acid
patent-application, December 2009

Carboxylic Acids Prepared Using a Salt-Splitting Process
patent-application, April 2010

Biological Synthesis Of Difunctional Alkanes From Carbohydrate Feedstocks
patent-application, June 2010

Compositions and Methods for Enhancing Tolerance for the Production of Organic Chemicals Produced by Microorganisms
patent-application, August 2010

Purification of aqueous solutions of organic acids
patent-application, April 2011

Production of an Organic Acid and/or Related Chemicals
patent-application, May 2011

Process for the Biological Production of 3-Hydroxypropionic Acid with High Yield
patent-application, June 2011

Methods, systems and compositions for increased microorganism tolerance to and production of 3-hydroxypropionic acid (3-HP)
patent-application, October 2011

Carboxylic acid recovery and methods related thereto
patent-application, November 2011

Compositions and methods for 3-hydroxypropionate bio-production from biomass
patent-application, February 2012

3-Hydroxypropionic Acid and Other Organic Compounds
patent-application, September 2012

Methods, Systems And Compositions For Increased Microorganism Tolerance To And Production Of 3-Hydroxypropionic Acid (3-HP)
patent-application, October 2012

Methods for producing 3-hydroxypropionic acid and other products
patent-application, March 2013

Microorganism Production of High-Value Chemical Products, and Related Compositions, Methods and Systems
patent-application, May 2013

Methods, Systems And Compositions Related To Reduction Of Conversions Of Microbially Produced 3-Hydroxyproplonic Acid (3-HP) To Aldehyde Metabolites
patent-application, July 2013

Purification Of 3-Hydroxypropionic Acid From Crude Cell Broth And Production Of Acrylamide
patent-application, December 2013

Purification of 3-Hydroxypropionic Acid from Crude Cell Broth and Production of Acrylamide
patent-application, October 2014

Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase
journal, January 1988

A New Enzymatic Method of Acrylamide Production
journal, May 1982

Inverse metabolic engineering: A strategy for directed genetic engineering of useful phenotypes
journal, July 2002

Induction of specific base-pair substitutions in E. coli trpA mutants by chloroenthylene oxide, a carcinogenic vinyl chloride metabolite
journal, November 1985

The biological degradation of cellulose
journal, January 1994

Sequences of the envM gene and of two mutated alleles in Escherichia coli
journal, October 1992

Naturally Occurring Adenines within mRNA Coding Sequences Affect Ribosome Binding and Expression in Escherichia coli
journal, November 2006

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998

Synthesis of labeled acrylamide andN-methylolacrylamide (NMA):15N-acrylamide,13C-NMA,15N-NMA, and13C,15N-NMA
journal, January 2005

The IdhA Gene Encoding the Fermentative Lactate Dehydrogenase of Escherichia Coli
journal, January 1997

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005

Simultaneous synthesis of enantiomerically pure (S)-amino acids and (R)-amines using coupled transaminase reactions
journal, January 2003

The commercial production of chemicals using pathway engineering
journal, December 2000

Inhibitory activity spectrum of reuterin produced by Lactobacillus reuteri against intestinal bacteria
journal, January 2007

DNA shuffling of a family of genes from diverse species accelerates directed evolution
journal, January 1998

Cloning, crystallization and preliminary characterization of a β-carbonic anhydrase from Escherichia coli
journal, September 2000

Thermal regulation of membrane lipid fluidity in bacteria
journal, February 1983

Practical limits of function prediction
journal, January 2000

Engineering of a functional human NADH-dependent cytochrome P450 system
journal, January 2001

Structure of the EntB Multidomain Nonribosomal Peptide Synthetase and Functional Analysis of Its Interaction with the EntE Adenylation Domain
journal, April 2006

Lactate-Utilizing Bacteria, Isolated from Human Feces, That Produce Butyrate as a Major Fermentation Product
journal, October 2004

The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase
journal, September 1986

Switch of coenzyme specificity of p -hydroxybenzoate hydroxylase 1 1Edited by A. R. Fersht
journal, September 1999

Improving lycopene production in Escherichia coli by engineering metabolic control
journal, May 2000

Biorefineries:  Current Status, Challenges, and Future Direction
journal, July 2006

A Novel Quinone-forming Monooxygenase Family Involved in Modification of Aromatic Polyketides
journal, February 2005

Genome-wide screening for trait conferring genes using DNA microarrays
journal, May 2002

Identification and cloning of the Mycobacterium avium folA gene, required for dihydrofolate reductase activity
journal, November 1997

Overproduction of phage Lambda repressor under control of the lac promotor of Escherichia coli
journal, January 1976

Genetic Evidence for an Interaction of the UbiG O-Methyltransferase with UbiX in Escherichia coli Coenzyme Q Biosynthesis
journal, August 2006

Exploring the diversity of complex metabolic networks
journal, December 2004

A T42M Substitution in Bacterial 5-Enolpyruvylshikimate-3-phosphate Synthase (EPSPS) Generates Enzymes with Increased Resistance to Glyphosate
journal, January 2003

Biosynthetic pathways for 3-hydroxypropionic acid production
journal, February 2009

Purification and characterization of 2-oxoglutarate decarboxylase of Leuconostoc oenos
journal, August 1990

Engineering primary metabolic pathways of industrial micro-organisms
journal, March 2007

Extractive recovery of products from fermentation broths
journal, April 1999

The Rut Pathway for Pyrimidine Degradation: Novel Chemistry and Toxicity Problems
journal, April 2010

Dihydrolipoamide Dehydrogenase Mutation Alters the NADH Sensitivity of Pyruvate Dehydrogenase Complex of Escherichia coli K-12
journal, March 2008

Effect of Overexpression of Actinobacillus succinogenes Phosphoenolpyruvate Carboxykinase on Succinate Production in Escherichia coli
journal, February 2004

A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity
journal, January 2007

Polypeptide Release Factors in Prokaryotes and Eukaryotes
journal, January 2002

Reactions of β-lactones with potassium alkoxides and their complexes with 18-crown-6 in aprotic solvents
journal, July 1993

A Physiology Study of Escherichia coli Overexpressing Phosphoenolpyruvate Carboxykinase
journal, April 2008

A new preparation of trifluoromethanesulfinate salts
journal, July 2007

A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes
journal, June 2010

Effect of poxB gene knockout on metabolism in Escherichia coli based on growth characteristics and enzyme activities
journal, September 2006

Fe2(SO4)3·4H2O/concentrated H2SO4: an efficient catalyst for esterification
journal, March 2004

SCALEs: multiscale analysis of library enrichment
journal, November 2006

Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
journal, June 2003

Aminotransferases: demonstration of homology and division into evolutionary subgroups
journal, June 1993

Effect of process parameters on 3-hydroxypropionic acid production from glycerol using a recombinant Escherichia coli
journal, April 2009

Human Catechol-O-Methyltransferase Haplotypes Modulate Protein Expression by Altering mRNA Secondary Structure
journal, December 2006

A novel methodology employing Corynebacterium glutamicum genome information to generate a new L -lysine-producing mutant
journal, January 2002

Unprecedented acetoacetyl-coenzyme A synthesizing enzyme of the thiolase superfamily involved in the mevalonate pathway
journal, June 2010

Cloning and Overexpression in Soluble Form of Functional Shikimate Kinase and 5-Enolpyruvylshikimate 3-Phosphate Synthase Enzymes from Mycobacterium tuberculosis
journal, August 2001

Metabolic engineering of aromatic group amino acid pathway in Bacillus subtilis for L-phenylalanine production
journal, November 2004

Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli
journal, January 2006

Genome shuffling of Lactobacillus for improved acid tolerance
journal, July 2002

A new perspective on thiamine catalysis
journal, August 2004

Polyphosphate Kinase Protects Salmonella enterica from Weak Organic Acid Stress
journal, April 2005

Effect of process parameters on 3-hydroxypropionic acid production from glycerol using a recombinant Escherichia coli
journal, April 2009

A mild and efficient method for esterification and transesterification catalyzed by iodine
journal, January 2002

Structure−Cytoprotective Activity Relationship of Simple Molecules Containing an α,β-Unsaturated Carbonyl System
journal, June 1997

Silent Polymorphisms Speak: How They Affect Pharmacogenomics and the Treatment of Cancer
journal, October 2007

Molecular breeding of carotenoid biosynthetic pathways
journal, July 2000

Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001

Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007

CHEMISTRY: Sugary Recipe Boosts Grow-Your-Own Plastics
journal, June 2006

Genes restoring redox balance in fermentation-deficient E. coli NZN111
journal, November 2009

Challenges in Engineering Microbes for Biofuels Production
journal, February 2007

Feasibility of acrylic acid production by fermentation
journal, February 2005

Variant tricarboxylic acid cycle in Mycobacterium tuberculosis: Identification of α-ketoglutarate decarboxylase
journal, July 2005

3-phenylpropionate catabolism and the Escherichia coli oxidative stress response
journal, April 2005

A genomics approach to improve the analysis and design of strain selections
journal, May 2008

Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli
journal, January 2012

Rapid dissection of a complex phenotype through genomic-scale mapping of fitness altering genes
journal, May 2010

Fermentation of Glucose to Lactic Acid Coupled with Reactive Extraction:  A Review
journal, September 2004

A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase
journal, November 1995

Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999

On the Role of Helix 0 of the Tryptophan Synthetase α Chain of Escherichia coli
journal, June 1996

Identification of PhoB binding sites of the yibD and ytfK promoter regions in Escherichia coli
journal, April 2011

Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering
journal, May 2009

Binding of Two Flaviolin Substrate Molecules, Oxidative Coupling, and Crystal Structure of Streptomyces coelicolor A3(2) Cytochrome P450 158A2
journal, January 2005

Interdomain Communication between the Thiolation and Thioesterase Domains of EntF Explored by Combinatorial Mutagenesis and Selection
journal, August 2006

A core catalytic domain of the TyrA protein family: arogenate dehydrogenase from Synechocystis
journal, August 2004

Selective production of L-aspartic acid and L-phenylalanine by coupling reactions of aspartase and aminotransferase in Escherichia coli
journal, July 2000

FolM, A New Chromosomally Encoded Dihydrofolate Reductase in Escherichia coli
journal, November 2003

The qmeA (ts) mutation of Escherichia coli is localized in the fabI gene, which encodes enoyl-ACP reductase
journal, January 1996

Characterization of two temperature-inducible promoters newly isolated from B. subtilis
journal, July 2007

De novo biosynthetic pathways: rational design of microbial chemical factories
journal, October 2008

Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli
journal, December 2002

Inhibiting Bacterial Fatty Acid Synthesis
journal, April 2006

?-hydroxypropionic acid production by Byssochlamys sp. grown on acrylic acid
journal, November 1993