skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Recoding method that removes inhibitory sequences and improves HIV gene expression

Abstract

The invention relates to inhibitory nucleotide signal sequences or "INS" sequences in the genomes of lentiviruses. In particular the invention relates to the AGG motif present in all viral genomes. The AGG motif may have an inhibitory effect on a virus, for example by reducing the levels of, or maintaining low steady-state levels of, viral RNAs in host cells, and inducing and/or maintaining in viral latency. In one aspect, the invention provides vaccines that contain, or are produced from, viral nucleic acids in which the AGG sequences have been mutated. In another aspect, the invention provides methods and compositions for affecting the function of the AGG motif, and methods for identifying other INS sequences in viral genomes.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Institute of Advanced Study, Princeton, NJ (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1303730
Patent Number(s):
9,422,342
Application Number:
12/373,605
Assignee:
Institute of Advanced Study (Princeton, NJ)
DOE Contract Number:  
FG02-90ER40542
Resource Type:
Patent
Resource Relation:
Patent File Date: 2007 Jul 12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Rabadan, Raul, Krasnitz, Michael, Robins, Harlan, Witten, Daniela, and Levine, Arnold. Recoding method that removes inhibitory sequences and improves HIV gene expression. United States: N. p., 2016. Web.
Rabadan, Raul, Krasnitz, Michael, Robins, Harlan, Witten, Daniela, & Levine, Arnold. Recoding method that removes inhibitory sequences and improves HIV gene expression. United States.
Rabadan, Raul, Krasnitz, Michael, Robins, Harlan, Witten, Daniela, and Levine, Arnold. Tue . "Recoding method that removes inhibitory sequences and improves HIV gene expression". United States. https://www.osti.gov/servlets/purl/1303730.
@article{osti_1303730,
title = {Recoding method that removes inhibitory sequences and improves HIV gene expression},
author = {Rabadan, Raul and Krasnitz, Michael and Robins, Harlan and Witten, Daniela and Levine, Arnold},
abstractNote = {The invention relates to inhibitory nucleotide signal sequences or "INS" sequences in the genomes of lentiviruses. In particular the invention relates to the AGG motif present in all viral genomes. The AGG motif may have an inhibitory effect on a virus, for example by reducing the levels of, or maintaining low steady-state levels of, viral RNAs in host cells, and inducing and/or maintaining in viral latency. In one aspect, the invention provides vaccines that contain, or are produced from, viral nucleic acids in which the AGG sequences have been mutated. In another aspect, the invention provides methods and compositions for affecting the function of the AGG motif, and methods for identifying other INS sequences in viral genomes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {8}
}

Patent:

Save / Share:

Works referenced in this record:

A Rev-Independent Human Immunodeficiency Virus Type 1 (HIV-1)-Based Vector That Exploits a Codon-Optimized HIV-1 gag-pol Gene
journal, May 2000


Suppression of HIV replication by dominant negative mutants of HIV-1 (review).
journal, September 1998

  • Inubushi, R.; Shimano, R.; Oshima, Y.
  • International Journal of Molecular Medicine
  • DOI: 10.3892/ijmm.2.3.325

Functional Surfaces of the Human Immunodeficiency Virus Type 1 Capsid Protein
journal, May 2003


A Recoding Method to Improve the Humoral Immune Response to an HIV DNA Vaccine
journal, September 2008


Increased Expression and Immunogenicity of Sequence-Modified Human Immunodeficiency Virus Type 1 gag Gene
journal, March 2000


A Relative-Entropy Algorithm for Genomic Fingerprinting Captures Host-Phage Similarities
journal, November 2005


Relative contributions of codon usage, promoter efficiency and leader sequence to the antigen expression and immunogenicity of HIV-1 Env DNA vaccine
journal, May 2006