Recoding method that removes inhibitory sequences and improves HIV gene expression
Abstract
The invention relates to inhibitory nucleotide signal sequences or "INS" sequences in the genomes of lentiviruses. In particular the invention relates to the AGG motif present in all viral genomes. The AGG motif may have an inhibitory effect on a virus, for example by reducing the levels of, or maintaining low steady-state levels of, viral RNAs in host cells, and inducing and/or maintaining in viral latency. In one aspect, the invention provides vaccines that contain, or are produced from, viral nucleic acids in which the AGG sequences have been mutated. In another aspect, the invention provides methods and compositions for affecting the function of the AGG motif, and methods for identifying other INS sequences in viral genomes.
- Inventors:
- Issue Date:
- Research Org.:
- Institute for Advanced Study, Princeton, NJ (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1303730
- Patent Number(s):
- 9422342
- Application Number:
- 12/373,605
- Assignee:
- Institute of Advanced Study (Princeton, NJ)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- DOE Contract Number:
- FG02-90ER40542
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2007 Jul 12
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES
Citation Formats
Rabadan, Raul, Krasnitz, Michael, Robins, Harlan, Witten, Daniela, and Levine, Arnold. Recoding method that removes inhibitory sequences and improves HIV gene expression. United States: N. p., 2016.
Web.
Rabadan, Raul, Krasnitz, Michael, Robins, Harlan, Witten, Daniela, & Levine, Arnold. Recoding method that removes inhibitory sequences and improves HIV gene expression. United States.
Rabadan, Raul, Krasnitz, Michael, Robins, Harlan, Witten, Daniela, and Levine, Arnold. Tue .
"Recoding method that removes inhibitory sequences and improves HIV gene expression". United States. https://www.osti.gov/servlets/purl/1303730.
@article{osti_1303730,
title = {Recoding method that removes inhibitory sequences and improves HIV gene expression},
author = {Rabadan, Raul and Krasnitz, Michael and Robins, Harlan and Witten, Daniela and Levine, Arnold},
abstractNote = {The invention relates to inhibitory nucleotide signal sequences or "INS" sequences in the genomes of lentiviruses. In particular the invention relates to the AGG motif present in all viral genomes. The AGG motif may have an inhibitory effect on a virus, for example by reducing the levels of, or maintaining low steady-state levels of, viral RNAs in host cells, and inducing and/or maintaining in viral latency. In one aspect, the invention provides vaccines that contain, or are produced from, viral nucleic acids in which the AGG sequences have been mutated. In another aspect, the invention provides methods and compositions for affecting the function of the AGG motif, and methods for identifying other INS sequences in viral genomes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {8}
}
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