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Title: Method for genetic identification of unknown organisms

Abstract

A method of rapid, genome and proteome based identification of unknown pathogenic or non-pathogenic organisms in a complex sample. The entire sample is analyzed by creating millions of emulsion encapsulated microdroplets, each containing a single pathogenic or non-pathogenic organism sized particle and appropriate reagents for amplification. Following amplification, the amplified product is analyzed.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1303664
Patent Number(s):
9,422,586
Application Number:
11/818,623
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Patent File Date: 2007 Jun 15
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Colston, Jr., Billy W., Fitch, Joseph P., Hindson, Benjamin J., Carter, Chance J., and Beer, Neil Reginald. Method for genetic identification of unknown organisms. United States: N. p., 2016. Web.
Colston, Jr., Billy W., Fitch, Joseph P., Hindson, Benjamin J., Carter, Chance J., & Beer, Neil Reginald. Method for genetic identification of unknown organisms. United States.
Colston, Jr., Billy W., Fitch, Joseph P., Hindson, Benjamin J., Carter, Chance J., and Beer, Neil Reginald. Tue . "Method for genetic identification of unknown organisms". United States. https://www.osti.gov/servlets/purl/1303664.
@article{osti_1303664,
title = {Method for genetic identification of unknown organisms},
author = {Colston, Jr., Billy W. and Fitch, Joseph P. and Hindson, Benjamin J. and Carter, Chance J. and Beer, Neil Reginald},
abstractNote = {A method of rapid, genome and proteome based identification of unknown pathogenic or non-pathogenic organisms in a complex sample. The entire sample is analyzed by creating millions of emulsion encapsulated microdroplets, each containing a single pathogenic or non-pathogenic organism sized particle and appropriate reagents for amplification. Following amplification, the amplified product is analyzed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {8}
}

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Works referenced in this record:

Amplification of complex gene libraries by emulsion PCR
journal, July 2006

  • Williams, Richard; Peisajovich, Sergio G.; Miller, Oliver J.
  • Nature Methods, Vol. 3, Issue 7, p. 545-550
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APPLIED PHYSICS: Droplet Control for Microfluidics
journal, August 2005


Selective Encapsulation of Single Cells and Subcellular Organelles into Picoliter- and Femtoliter-Volume Droplets
journal, March 2005

  • He, Mingyan; Edgar, J. Scott; Jeffries, Gavin D. M.
  • Analytical Chemistry, Vol. 77, Issue 6
  • DOI: 10.1021/ac0480850

Single-molecule PCR using water-in-oil emulsion
journal, April 2003

  • Nakano, Michihiko; Komatsu, Jun; Matsuura, Shun-ichi
  • Journal of Biotechnology, Vol. 102, Issue 2, p. 117-124
  • DOI: 10.1016/S0168-1656(03)00023-3