Identification of discriminant proteins through antibody profiling, methods and apparatus for identifying an individual

Title: Identification of discriminant proteins through antibody profiling, methods and apparatus for identifying an individual

A method for determining a plurality of proteins for discriminating and positively identifying an individual based from a biological sample. The method may include profiling a biological sample from a plurality of individuals against a protein array including a plurality of proteins. The protein array may include proteins attached to a support in a preselected pattern such that locations of the proteins are known. The biological sample may be contacted with the protein array such that a portion of antibodies in the biological sample reacts with and binds to the proteins forming immune complexes. A statistical analysis method, such as discriminant analysis, may be performed to determine discriminating proteins for distinguishing individuals. Proteins of interest may be used to form a protein array. Such a protein array may be used, for example, to compare a forensic sample from an unknown source with a sample from a known source.

Inventors:: Apel, William A.; Thompson, Vicki S; Lacey, Jeffrey A.; Gentillon, Cynthia A.

Issue Date:: 2016-08-09

OSTI Identifier:: 1290004

Assignee:: Battelle Energy Alliance, LLC (Idaho Falls, ID) INL

Patent Number(s):: 9,410,965

Application Number:: 12/883,002

Contract Number:: AC07-05ID14517

Resource Relation:: Patent File Date: 2010 Sep 15

Research Org:: Idaho National Lab. (INL), Idaho Falls, ID (United States)

Sponsoring Org:: USDOE

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Full Text
View Full Text

Have feedback or suggestions for a way to improve these results? !

Save / Share this Record no
Export Metadata
- Endnote
- RIS
- Excel
- CSV
- XML
Send to Email

Send to Email

Email address:

Content:

Works referenced in this record:

Detection of analyte binding to microarrays using gold nanoparticle labels and a desktop scanner
journal, September 2003

Han, Anpan; Dufva, Martin; Belleville, Erik
Lab on a Chip, Vol. 3, Issue 4, p. 329-332
DOI: 10.1039/B310814G

Electrogenerated Chemiluminescence: An Oxidative-Reduction Type ECL Reaction Sequence Using Tripropyl Amine
journal, January 1990

Leland, Jonathan K.; Powell, Michael J.
Journal of The Electrochemical Society, Vol. 137, Issue 10, p. 3127-3131
DOI: 10.1149/1.2086171

Cloning of open reading frames and promoters from the Saccharomyces cerevisiae genome: construction of genomic libraries of random small fragments
journal, January 1986

Santangelo, George M.; Tornow, Joanne; Moldave, Kivie
Gene, Vol. 46, Issue 2-3, p. 181-186
DOI: 10.1016/0378-1119(86)90402-6

Antibody profiling as an identification tool for forensic samples
conference, February 1999

Thompson, Vicki S.; Barrett, Karen B.; Davis, Tilton
Enabling Technologies for Law Enforcement and Security, vp.
DOI: 10.1117/12.334545

Fiber-Optic Immunosensor for the Detection of Fumonisin B₁
journal, April 1996

Thompson, Vicki S.; Maragos, Chris M.
Journal of Agricultural and Food Chemistry, Vol. 44, Issue 4, p. 1041-1046
DOI: 10.1021/jf950661t

Preliminary Investigations into Tris(2,2'-bipyridyl) Ruthenium (III) as a Chemiluminescent Reagent for the Detection of 3,6-Diacetylmorphine (Heroin) on Surfaces
journal, September 2007

Agg, Kent M.; Barnett, Neil W.; Lewis, Simon W.
Journal of Forensic Sciences, Vol. 52, Issue 5, p. 1111-1114
DOI: 10.1111/j.1556-4029.2007.00424.x

Difference gel electrophoresis. A single gel method for detecting changes in protein extracts
journal, January 1997

Ünlü, Mustafa; Morgan, Mary E.; Minden, Jonathan S.
Electrophoresis, Vol. 18, Issue 11, p. 2071-2077
DOI: 10.1002/elps.1150181133

Micromosaic Immunoassays
journal, November 2000

Bernard, André; Michel, Bruno; Delamarche, Emmanuel
Analytical Chemistry, Vol. 73, Issue 1, p. 8-12
DOI: 10.1021/ac0008845

Saliva Testing for Drugs of Abuse
journal, September 1993

Cone, Edward J.
Annals of the New York Academy of Sciences, Vol. 694, Issue 1, p. 91-127
DOI: 10.1111/j.1749-6632.1993.tb18346.x

Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale
journal, October 1997

DeRisi, J. L.; Iyer, Vishwanath R.; Brown, Patrick O.
Science, Vol. 278, Issue 5338, p. 680-686
DOI: 10.1126/science.278.5338.680

Automatic multiparameter fluorescence imaging for determining lymphocyte phenotype and activation status in melanoma tissue sections
journal, September 1996

Dow, Alasdair I.; Shafer, Steven A.; Kirkwood, John M.
Cytometry, Vol. 25, Issue 1, p. 71-81
DOI: 10.1002/(SICI)1097-0320(19960901)25:1<71::AID-CYTO8>3.0.CO;2-H

Advances in ELISA and other solid-phase immunoassays
journal, March 1986

Kemeny, D. M.; Challacombe, S. J.
Immunology Today, Vol. 7, Issue 3, p. 67-68
DOI: 10.1016/0167-5699(86)90084-8

Testing for drugs of abuse in saliva and sweat
journal, August 1998

Kidwell, David A.; Holland, Janel C.; Athanaselis, Sotiris
Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 713, Issue 1, p. 111-135
DOI: 10.1016/S0378-4347(97)00572-0

Multilayer peroxidase-labeled antibody method: Comparison with labeled streptavidin-biotin method, avidin-biotin-peroxidase complex method, and peroxidase-antiperoxidase method
journal, January 1995

Shin, Masashi; Izumi, Shin-Ichi; Nakane, Paul K.
Journal of Clinical Laboratory Analysis, Vol. 9, Issue 6, p. 424-430
DOI: 10.1002/jcla.1860090615

Rapid Detection and Enumeration of Legionella pneumophila in Hot Water Systems by Solid-Phase Cytometry
journal, March 2004

Aurell, H.; Catala, P.; Farge, P.
Applied and Environmental Microbiology, Vol. 70, Issue 3, p. 1651-1657
DOI: 10.1128/AEM.70.3.1651-1657.2004

Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions
journal, January 2001

Haab, Brian B.; Dunham, Maitreya J.; Brown, Patrick O.
Genome Biology, Vol. 2, Issue 2, Article No. research0004.1
DOI: 10.1186/gb-2001-2-2-research0004

Pattern Recognition in Pulmonary Tuberculosis Defined by High Content Peptide Microarray Chip Analysis Representing 61 Proteins from M. tuberculosis
journal, December 2008

Gaseitsiwe, Simani; Valentini, Davide; Mahdavifar, Shahnaz
PLoS ONE, Vol. 3, Issue 12, Article No. e3840
DOI: 10.1371/journal.pone.0003840

Similar Records

Similar records in DOepatents and OSTI.GOV collections:

Identification of discriminant proteins through antibody profiling, methods and apparatus for identifying an individual

Patent Thompson, Vicki S ; Lacey, Jeffrey A ; Gentillon, Cynthia A ; Apel, William A

A method for determining a plurality of proteins for discriminating and positively identifying an individual based from a biological sample. The method may include profiling a biological sample from a plurality of individuals against a protein array including a plurality of proteins. The protein array may include proteins attached to a support in a preselected pattern such that locations of the proteins are known. The biological sample may be contacted with the protein array such that a portion of antibodies in the biological sample reacts with and binds to the proteins forming immune complexes. A statistical analysis method, such asmore »« less
Full Text Available March 2015
Individual-specific antibody identification methods

Patent Francoeur, Ann -Michele

An identification method, applicable to the identification of animals or inanimate objects, is described. The method takes advantage of a hithertofore unknown set of individual-specific, or IS antibodies, that are part of the unique antibody repertoire present in animals, by reacting an effective amount of IS antibodies with a particular panel, or n-dimensional array (where n is typically one or two) consisting of an effective amount of many different antigens (typically greater than one thousand), to give antibody-antigen complexes. The profile or pattern formed by the antigen-antibody complexes, termed an antibody fingerprint, when revealed by an effective amount of anmore »« less
Full Text Available November 1989
Antibody profiling sensitivity through increased reporter antibody layering

Patent Apel, William A ; Thompson, Vicki S

A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immunemore »« less
Full Text Available February 2013
Antibody profiling sensitivity through increased reporter antibody layering

Patent Apel, William A. ; Thompson, Vicki S

A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immunemore »« less
Full Text Available April 2010
Antibody profiling sensitivity through increased reporter antibody layering

Patent Apel, William A. ; Thompson, Vicki S.

A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immunemore »« less
Full Text Available February 2013