skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Identification of discriminant proteins through antibody profiling, methods and apparatus for identifying an individual

Abstract

A method for determining a plurality of proteins for discriminating and positively identifying an individual based from a biological sample. The method may include profiling a biological sample from a plurality of individuals against a protein array including a plurality of proteins. The protein array may include proteins attached to a support in a preselected pattern such that locations of the proteins are known. The biological sample may be contacted with the protein array such that a portion of antibodies in the biological sample reacts with and binds to the proteins forming immune complexes. A statistical analysis method, such as discriminant analysis, may be performed to determine discriminating proteins for distinguishing individuals. Proteins of interest may be used to form a protein array. Such a protein array may be used, for example, to compare a forensic sample from an unknown source with a sample from a known source.

Inventors:
; ; ;
Issue Date:
Research Org.:
Idaho National Lab. (INL), Idaho Falls, ID (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1290004
Patent Number(s):
9,410,965
Application Number:
12/883,002
Assignee:
Battelle Energy Alliance, LLC (Idaho Falls, ID)
DOE Contract Number:  
AC07-05ID14517
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Sep 15
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Apel, William A., Thompson, Vicki S, Lacey, Jeffrey A., and Gentillon, Cynthia A. Identification of discriminant proteins through antibody profiling, methods and apparatus for identifying an individual. United States: N. p., 2016. Web.
Apel, William A., Thompson, Vicki S, Lacey, Jeffrey A., & Gentillon, Cynthia A. Identification of discriminant proteins through antibody profiling, methods and apparatus for identifying an individual. United States.
Apel, William A., Thompson, Vicki S, Lacey, Jeffrey A., and Gentillon, Cynthia A. Tue . "Identification of discriminant proteins through antibody profiling, methods and apparatus for identifying an individual". United States. https://www.osti.gov/servlets/purl/1290004.
@article{osti_1290004,
title = {Identification of discriminant proteins through antibody profiling, methods and apparatus for identifying an individual},
author = {Apel, William A. and Thompson, Vicki S and Lacey, Jeffrey A. and Gentillon, Cynthia A.},
abstractNote = {A method for determining a plurality of proteins for discriminating and positively identifying an individual based from a biological sample. The method may include profiling a biological sample from a plurality of individuals against a protein array including a plurality of proteins. The protein array may include proteins attached to a support in a preselected pattern such that locations of the proteins are known. The biological sample may be contacted with the protein array such that a portion of antibodies in the biological sample reacts with and binds to the proteins forming immune complexes. A statistical analysis method, such as discriminant analysis, may be performed to determine discriminating proteins for distinguishing individuals. Proteins of interest may be used to form a protein array. Such a protein array may be used, for example, to compare a forensic sample from an unknown source with a sample from a known source.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {8}
}

Patent:

Save / Share:

Works referenced in this record:

Antibody Fingerprinting: A Novel Method for Identifying Individual People and Animals
journal, July 1988


Detection of analyte binding to microarrays using gold nanoparticle labels and a desktop scanner
journal, September 2003

  • Han, Anpan; Dufva, Martin; Belleville, Erik
  • Lab on a Chip, Vol. 3, Issue 4, p. 329-332
  • DOI: 10.1039/B310814G

A new type of synthetic peptide library for identifying ligand-binding activity
journal, November 1991

  • Lam, Kit S.; Salmon, Sydney E.; Hersh, Evan M.
  • Nature, Vol. 354, Issue 6348
  • DOI: 10.1038/354082a0

Electrogenerated Chemiluminescence: An Oxidative-Reduction Type ECL Reaction Sequence Using Tripropyl Amine
journal, January 1990

  • Leland, Jonathan K.; Powell, Michael J.
  • Journal of The Electrochemical Society, Vol. 137, Issue 10, p. 3127-3131
  • DOI: 10.1149/1.2086171

Microarray technology: the future of blood testing?
journal, January 2001


Cloning of open reading frames and promoters from the Saccharomyces cerevisiae genome: construction of genomic libraries of random small fragments
journal, January 1986


Antibody profiling as an identification tool for forensic samples
conference, February 1999

  • Thompson, Vicki S.; Barrett, Karen B.; Davis, Tilton
  • Enabling Technologies for Law Enforcement and Security, vp.
  • DOI: 10.1117/12.334545

Fiber-Optic Immunosensor for the Detection of Fumonisin B1
journal, April 1996

  • Thompson, Vicki S.; Maragos, Chris M.
  • Journal of Agricultural and Food Chemistry, Vol. 44, Issue 4, p. 1041-1046
  • DOI: 10.1021/jf950661t

Yeast RNA polymerase II genes: isolation with antibody probes
journal, November 1983


Preliminary Investigations into Tris(2,2'-bipyridyl) Ruthenium (III) as a Chemiluminescent Reagent for the Detection of 3,6-Diacetylmorphine (Heroin) on Surfaces
journal, September 2007


Difference gel electrophoresis. A single gel method for detecting changes in protein extracts
journal, January 1997

  • Ünlü, Mustafa; Morgan, Mary E.; Minden, Jonathan S.
  • Electrophoresis, Vol. 18, Issue 11, p. 2071-2077
  • DOI: 10.1002/elps.1150181133

Micromosaic Immunoassays
journal, November 2000

  • Bernard, André; Michel, Bruno; Delamarche, Emmanuel
  • Analytical Chemistry, Vol. 73, Issue 1, p. 8-12
  • DOI: 10.1021/ac0008845

Athletic drug Testing
journal, April 1998


Saliva Testing for Drugs of Abuse
journal, September 1993


Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale
journal, October 1997


Advances in ELISA and other solid-phase immunoassays
journal, March 1986


Testing for drugs of abuse in saliva and sweat
journal, August 1998

  • Kidwell, David A.; Holland, Janel C.; Athanaselis, Sotiris
  • Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 713, Issue 1, p. 111-135
  • DOI: 10.1016/S0378-4347(97)00572-0

Multilayer peroxidase-labeled antibody method: Comparison with labeled streptavidin-biotin method, avidin-biotin-peroxidase complex method, and peroxidase-antiperoxidase method
journal, January 1995

  • Shin, Masashi; Izumi, Shin-Ichi; Nakane, Paul K.
  • Journal of Clinical Laboratory Analysis, Vol. 9, Issue 6, p. 424-430
  • DOI: 10.1002/jcla.1860090615

Rapid Detection and Enumeration of Legionella pneumophila in Hot Water Systems by Solid-Phase Cytometry
journal, March 2004


A novel immunohistochemical technique for demonstration of specific binding of human monoclonal antibodies to human cryostat tissue sections.
journal, July 1990

  • Tuson, J. R.; Pascoe, E. W.; Jacob, D. A.
  • Journal of Histochemistry & Cytochemistry, Vol. 38, Issue 7
  • DOI: 10.1177/38.7.2355173

Primary Antibody–Fab Fragment Complexes: A Flexible Alternative to Traditional Direct and Indirect Immunolabeling Techniques
journal, September 2004

  • Brown, Jeremy K.; Pemberton, Alan D.; Wright, Steven H.
  • Journal of Histochemistry & Cytochemistry, Vol. 52, Issue 9
  • DOI: 10.1369/jhc.3A6200.2004

Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions
journal, January 2001


Pattern Recognition in Pulmonary Tuberculosis Defined by High Content Peptide Microarray Chip Analysis Representing 61 Proteins from M. tuberculosis
journal, December 2008

  • Gaseitsiwe, Simani; Valentini, Davide; Mahdavifar, Shahnaz
  • PLoS ONE, Vol. 3, Issue 12, Article No. e3840
  • DOI: 10.1371/journal.pone.0003840

Development of an individual-specific autoantibody (ISA) protein microarray
journal, February 2005

  • Lebrun, Stewart J.; Suquilanda, Edgar; Petchpud, Wasinee N.
  • Biotechnology and Applied Biochemistry, Vol. 41, Issue 1
  • DOI: 10.1042/BA20040026