skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Devices, systems, and methods for microscale isoelectric fractionation

Abstract

Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.

Inventors:
; ; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1289918
Patent Number(s):
9,409,357
Application Number:
13/747,587
Assignee:
Sandia Corporation (Albuquerque, NM)
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Jan 23
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION

Citation Formats

Sommer, Gregory J., Hatch, Anson V., Wang, Ying-Chih, and Singh, Anup K. Devices, systems, and methods for microscale isoelectric fractionation. United States: N. p., 2016. Web.
Sommer, Gregory J., Hatch, Anson V., Wang, Ying-Chih, & Singh, Anup K. Devices, systems, and methods for microscale isoelectric fractionation. United States.
Sommer, Gregory J., Hatch, Anson V., Wang, Ying-Chih, and Singh, Anup K. Tue . "Devices, systems, and methods for microscale isoelectric fractionation". United States. https://www.osti.gov/servlets/purl/1289918.
@article{osti_1289918,
title = {Devices, systems, and methods for microscale isoelectric fractionation},
author = {Sommer, Gregory J. and Hatch, Anson V. and Wang, Ying-Chih and Singh, Anup K.},
abstractNote = {Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {8}
}

Patent:

Save / Share:

Works referenced in this record:

On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
journal, May 2008

  • Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V.
  • Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333
  • DOI: 10.1021/ac702523g

Integrated Preconcentration SDS−PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels
journal, July 2006

  • Hatch, Anson V.; Herr, Amy E.; Throckmorton, Daniel J.
  • Analytical Chemistry, Vol. 78, Issue 14, p. 4976-4984
  • DOI: 10.1021/ac0600454