Devices, systems, and methods for microscale isoelectric fractionation
Abstract
Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.
- Inventors:
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1289918
- Patent Number(s):
- 9409357
- Application Number:
- 13/747,587
- Assignee:
- Sandia Corporation (Albuquerque, NM)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B29 - WORKING OF PLASTICS B29D - PRODUCING PARTICULAR ARTICLES FROM PLASTICS OR FROM SUBSTANCES IN A PLASTIC STATE
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2013 Jan 23
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION
Citation Formats
Sommer, Gregory J., Hatch, Anson V., Wang, Ying-Chih, and Singh, Anup K. Devices, systems, and methods for microscale isoelectric fractionation. United States: N. p., 2016.
Web.
Sommer, Gregory J., Hatch, Anson V., Wang, Ying-Chih, & Singh, Anup K. Devices, systems, and methods for microscale isoelectric fractionation. United States.
Sommer, Gregory J., Hatch, Anson V., Wang, Ying-Chih, and Singh, Anup K. Tue .
"Devices, systems, and methods for microscale isoelectric fractionation". United States. https://www.osti.gov/servlets/purl/1289918.
@article{osti_1289918,
title = {Devices, systems, and methods for microscale isoelectric fractionation},
author = {Sommer, Gregory J. and Hatch, Anson V. and Wang, Ying-Chih and Singh, Anup K.},
abstractNote = {Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 09 00:00:00 EDT 2016},
month = {Tue Aug 09 00:00:00 EDT 2016}
}
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Works referenced in this record:
Multidimensional electrophoresis and methods of making and using thereof
patent-application, June 2004
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On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
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Integrated Preconcentration SDSāPAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels
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