Global to push GA events into
skip to main content

Title: Devices, systems, and methods for microscale isoelectric fractionation

Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.
Inventors:
; ; ;
Issue Date:
OSTI Identifier:
1289918
Assignee:
Sandia Corporation (Albuquerque, NM) SNL-A
Patent Number(s):
9,409,357
Application Number:
13/747,587
Contract Number:
AC04-94AL85000
Resource Relation:
Patent File Date: 2013 Jan 23
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION

Works referenced in this record:

On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
journal, May 2008
  • Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V.
  • Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333
  • DOI: 10.1021/ac702523g

Integrated Preconcentration SDSāˆ’PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels
journal, July 2006
  • Hatch, Anson V.; Herr, Amy E.; Throckmorton, Daniel J.
  • Analytical Chemistry, Vol. 78, Issue 14, p. 4976-4984
  • DOI: 10.1021/ac0600454