Systems and methods for the secretion of recombinant proteins in gram negative bacteria
Abstract
Disclosed herein are systems and methods for producing recombinant proteins utilizing mutant E. coli strains containing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the culture medium. Host cells transformed with the expression vectors are also provided.
- Inventors:
- Issue Date:
- Research Org.:
- WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1289850
- Patent Number(s):
- 9410157
- Application Number:
- 13/192,058
- Assignee:
- WISCONSIN ALUMNI RESEARCH FOUNDATION (Madison, WI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FC02-07ER64494
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2011 Jul 27
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Withers, III, Sydnor T., Dominguez, Miguel A, DeLisa, Matthew P., and Haitjema, Charles H. Systems and methods for the secretion of recombinant proteins in gram negative bacteria. United States: N. p., 2016.
Web.
Withers, III, Sydnor T., Dominguez, Miguel A, DeLisa, Matthew P., & Haitjema, Charles H. Systems and methods for the secretion of recombinant proteins in gram negative bacteria. United States.
Withers, III, Sydnor T., Dominguez, Miguel A, DeLisa, Matthew P., and Haitjema, Charles H. Tue .
"Systems and methods for the secretion of recombinant proteins in gram negative bacteria". United States. https://www.osti.gov/servlets/purl/1289850.
@article{osti_1289850,
title = {Systems and methods for the secretion of recombinant proteins in gram negative bacteria},
author = {Withers, III, Sydnor T. and Dominguez, Miguel A and DeLisa, Matthew P. and Haitjema, Charles H.},
abstractNote = {Disclosed herein are systems and methods for producing recombinant proteins utilizing mutant E. coli strains containing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the culture medium. Host cells transformed with the expression vectors are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 09 00:00:00 EDT 2016},
month = {Tue Aug 09 00:00:00 EDT 2016}
}
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Works referenced in this record:
Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli
journal, December 2005
- Zhang, Guijin; Brokx, Stephen; Weiner, Joel H
- Nature Biotechnology, Vol. 24, Issue 1, p. 100-104