skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Ketol-acid reductoisomerase enzymes and methods of use

Inventors:
; ; ; ; ; ;
Issue Date:
Research Org.:
Butamax Advanced Biofuels LLC, Wilmington, DE (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1261630
Patent Number(s):
9,388,392
Application Number:
14/853,108
Assignee:
Butamax Advanced Biofuels LLC (Wilmington, DE)
DOE Contract Number:  
AR0000006
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Sep 14
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Govindarajan, Sridhar, Li, Yougen, Liao, Der-Ing, O'Keefe, Daniel P., Minshull, Jeremy Stephen, Rothman, Steven Cary, and Tobias, Alexander Vincent. Ketol-acid reductoisomerase enzymes and methods of use. United States: N. p., 2016. Web.
Govindarajan, Sridhar, Li, Yougen, Liao, Der-Ing, O'Keefe, Daniel P., Minshull, Jeremy Stephen, Rothman, Steven Cary, & Tobias, Alexander Vincent. Ketol-acid reductoisomerase enzymes and methods of use. United States.
Govindarajan, Sridhar, Li, Yougen, Liao, Der-Ing, O'Keefe, Daniel P., Minshull, Jeremy Stephen, Rothman, Steven Cary, and Tobias, Alexander Vincent. Tue . "Ketol-acid reductoisomerase enzymes and methods of use". United States. https://www.osti.gov/servlets/purl/1261630.
@article{osti_1261630,
title = {Ketol-acid reductoisomerase enzymes and methods of use},
author = {Govindarajan, Sridhar and Li, Yougen and Liao, Der-Ing and O'Keefe, Daniel P. and Minshull, Jeremy Stephen and Rothman, Steven Cary and Tobias, Alexander Vincent},
abstractNote = {},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {7}
}

Patent:

Save / Share:

Works referenced in this record:

Carbon and electron flow in Clostridium butyricum grown in chemostat culture on glycerol and on glucose
journal, May 1996


Contribution of VanY D,D-carboxypeptidase to glycopeptide resistance in Enterococcus faecalis by hydrolysis of peptidoglycan precursors.
journal, September 1994

  • Arthur, M.; Depardieu, F.; Snaith, H. A.
  • Antimicrobial Agents and Chemotherapy, Vol. 38, Issue 9
  • DOI: 10.1128/AAC.38.9.1899

Targeted Disruption of the kstD Gene Encoding a 3-Ketosteroid Delta 1-Dehydrogenase Isoenzyme of Rhodococcus erythropolis Strain SQ1
journal, May 2000


Mechanism of ketol acid reductoisomerase. Steady-state analysis and metal ion requirement
journal, January 1989

  • Chunduru, Srinivas K.; Mrachko, Gregory T.; Calvo, K. C.
  • Biochemistry, Vol. 28, Issue 2
  • DOI: 10.1021/bi00428a012

Mycobacterium sp.,Rhodococcus erythropolis, andPseudomonas putida behavior in the presence of organic solvents
journal, January 2004

  • De Carvalho, Carla C. C. R.; Da Cruz, Alexandra A. R. L.; Pons, Marie-N�elle
  • Microscopy Research and Technique, Vol. 64, Issue 3
  • DOI: 10.1002/jemt.20061

Biochemical and molecular characterization of α-ketoisovalerate decarboxylase, an enzyme involved in the formation of aldehydes from amino acids by Lactococcus lactis
journal, September 2004


Ethanol production from cellulose by coupled saccharification/fermentation usingSaccharomyces cerevisiae and cellulase complex fromSclerotiun rolfsii UV-8 mutant
journal, September 1992

  • Deshpande, Mukund V.
  • Applied Biochemistry and Biotechnology, Vol. 36, Issue 3, p. 227-234
  • DOI: 10.1007/BF02921781

Solventogenic enzymes of Clostridium acetobutylicum: catalytic properties, genetic organization, and transcriptional regulation
journal, October 1995


Characterization of Ypr1p fromSaccharomyces cerevisiae as a 2-methylbutyraldehyde reductase
journal, January 2002

  • Ford, Gordon; Ellis, Elizabeth M.
  • Yeast, Vol. 19, Issue 12
  • DOI: 10.1002/yea.899

Technologies for butanol recovery integrated with fermentations
journal, March 1992

  • Groot, W. J.; van der Lans, R. G. J. M.; Luyben, K. Ch. A. M.
  • Process Biochemistry, Vol. 27, Issue 2, p. 61-75
  • DOI: 10.1016/0032-9592(92)80012-R

SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling
journal, January 1997


Improved methods for building protein models in electron density maps and the location of errors in these models
journal, March 1991

  • Jones, T. A.; Zou, J. Y.; Cowan, S. W.
  • Acta Crystallographica Section A Foundations of Crystallography, Vol. 47, Issue 2, p. 110-119
  • DOI: 10.1107/S0108767390010224

Effect of aliphatic alcohols on growth and degree of saturation of membrane lipids in Acinetobacter calcoaceticus
journal, March 2003

  • Kabelitz, Nadja; Santos, Pedro M.; Heipieper, Hermann J.
  • FEMS Microbiology Letters, Vol. 220, Issue 2, p. 223-227
  • DOI: 10.1016/S0378-1097(03)00103-4

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000

  • Datsenko, K. A.; Wanner, B. L.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645
  • DOI: 10.1073/pnas.120163297

A small cryptic plasmid from Rhodococcus erythropolis: characterization and utility for gene expression
journal, July 2003

  • Kostichka, K.; Tao, L.; Bramucci, M.
  • Applied Microbiology and Biotechnology, Vol. 62, Issue 1
  • DOI: 10.1007/s00253-003-1242-6

Characterization of the Saccharomyces cerevisiae YMR318C (ADH6) gene product as a broad specificity NADPH-dependent alcohol dehydrogenase: relevance in aldehyde reduction
journal, January 2002

  • Larroy, Carol; Fernandez, M. Rosario; Gonzalez, Eva
  • Biochemical Journal, Vol. 361, Issue 1, p. 163-172
  • DOI: 10.1042/bj3610163

Modular expression and secretion vectors for Bacillus subtilis
journal, May 1992


High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening
journal, December 1993


Plasmid vectors for Gram-positive bacteria switching from high to low copy number
journal, January 1996


Sequences of versatile broad-host-range vectors of the RK2 family
journal, July 2003


Identification, Cloning, and Characterization of a Lactococcus lactis Branched-Chain  -Keto Acid Decarboxylase Involved in Flavor Formation
journal, January 2005

  • Smit, B. A.; van Hylckama Vlieg, J. E. T.; Engels, W. J. M.
  • Applied and Environmental Microbiology, Vol. 71, Issue 1
  • DOI: 10.1128/AEM.71.1.303-311.2005

Proliferation and metabolic significance of peroxisomes in Candida boidinii during growth on d-alanine or oleic acid as the sole carbon source
journal, April 1990

  • Sulter, G. J.; Waterham, H. R.; Goodman, J. M.
  • Archives of Microbiology, Vol. 153, Issue 5
  • DOI: 10.1007/BF00248431

Crystal Structure of E.coli Alcohol Dehydrogenase YqhD: Evidence of a Covalently Modified NADP Coenzyme
journal, September 2004

  • Sulzenbacher, Gerlind; Alvarez, Karine; van den Heuvel, Robert H. H.
  • Journal of Molecular Biology, Vol. 342, Issue 2, p. 489-502
  • DOI: 10.1016/j.jmb.2004.07.034

Construction of highly efficient E. coli expression systems containing low oxygen induced promoter and partition region
journal, February 2005

  • Liu, Tao; Chen, Jing-yu; Zheng, Zhong
  • Applied Microbiology and Biotechnology, Vol. 68, Issue 3
  • DOI: 10.1007/s00253-005-1913-6

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
journal, January 1994

  • Thompson, Julie D.; Higgins, Desmond G.; Gibson, Toby J.
  • Nucleic Acids Research, Vol. 22, Issue 22, p. 4673-4680
  • DOI: 10.1093/nar/22.22.4673

Transcriptional Analysis of Butanol Stress and Tolerance in Clostridium acetobutylicum
journal, March 2004


The crystal structure of a bacterial Class II ketol-acid reductoisomerase: Domain conservation and evolution
journal, December 2005

  • Tyagi, Rajiv; Duquerroy, Stephane; Navaza, Jorge
  • Protein Science, Vol. 14, Issue 12
  • DOI: 10.1110/ps.051791305

Functional Analysis of Three Plasmids from Lactobacillus plantarum
journal, March 2005


Purification and characterization of a fusion protein of plant acetohydroxy acid synthase and acetohydroxy acid isomeroreductase
journal, May 1997


Mevalonate and Nonmevalonate Pathways for the Biosynthesis of Isoprene Units
journal, January 2002

  • Kuzuyama, Tomohisa
  • Bioscience, Biotechnology, and Biochemistry, Vol. 66, Issue 8
  • DOI: 10.1271/bbb.66.1619

Fusel Alcohols Production in Beer Fermentation Processes
journal, January 1994


Guerbet condensation of methanol with n-propanol to isobutyl alcohol over heterogeneous bifunctional catalysts based on Mg–Al mixed oxides partially substituted by different metal components
journal, May 2005

  • Carlini, Carlo; Marchionna, Mario; Noviello, Marilena
  • Journal of Molecular Catalysis A: Chemical, Vol. 232, Issue 1-2, p. 13-20
  • DOI: 10.1016/j.molcata.2004.12.037

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension
journal, April 1989


An Investigation of the Metabolism of Valine to Isobutyl Alcohol in Saccharomyces cerevisiae
journal, October 1998

  • Dickinson, J. Richard; Harrison, Scott J.; Hewlins, Michael J. E.
  • Journal of Biological Chemistry, Vol. 273, Issue 40, p. 25751-25756
  • DOI: 10.1074/jbc.273.40.25751

Formation of ethanol and higher alcohols by immobilized zymomonas mobilis in continuous culture
journal, January 1991


Switch of coenzyme specificity of p -hydroxybenzoate hydroxylase 1 1Edited by A. R. Fersht
journal, September 1999

  • Eppink, Michel H. M.; Overkamp, Karin M.; Schreuder, Herman A.
  • Journal of Molecular Biology, Vol. 292, Issue 1
  • DOI: 10.1006/jmbi.1999.3015

Identifying determinants of NADPH specificity in Baeyer-Villiger monooxygenases: NADPH specificity in BVMOs
journal, May 2004


Towards a new interaction enzyme:coenzyme
journal, April 2005

  • Martínez-Júlvez, Marta; Tejero, Jesús; R. Peregrina, José
  • Biophysical Chemistry, Vol. 115, Issue 2-3, p. 219-224
  • DOI: 10.1016/j.bpc.2004.12.034

Reversal of the Nucleotide Specificity of Ketol Acid Reductoisomerase by Site-Directed Mutagenesis Identifies the NADPH Binding Site1
journal, February 1997

  • Rane, Madhavi J.; Calvo, K. C.
  • Archives of Biochemistry and Biophysics, Vol. 338, Issue 1
  • DOI: 10.1006/abbi.1996.9802

Crystal Structure of Class I Acetohydroxy Acid Isomeroreductase from Pseudomonas aeruginosa
journal, April 2003


Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5
journal, June 2005

  • Paulsen, Ian T.; Press, Caroline M.; Ravel, Jacques
  • Nature Biotechnology, Vol. 23, Issue 7
  • DOI: 10.1038/nbt1110

NADP-Dependent enzymes. I: Conserved stereochemistry of cofactor binding
journal, May 1997


Enzymology, Structure, and Dynamics of Acetohydroxy Acid Isomeroreductase
journal, May 2001

  • Dumas, Renaud; Biou, Valérie; Halgand, Frédéric
  • Accounts of Chemical Research, Vol. 34, Issue 5
  • DOI: 10.1021/ar000082w

Modification of the Nucleotide Cofactor-binding Site of Cytochrome P-450 Reductase to Enhance Turnover with NADH in Vivo
journal, October 2002

  • Elmore, C. Lee; Porter, Todd D.
  • Journal of Biological Chemistry, Vol. 277, Issue 50
  • DOI: 10.1074/jbc.M210173200

The X-ray structure of Brassica napus β-keto acyl carrier protein reductase and its implications for substrate binding and catalysis
journal, April 2000


Computational design of Candida boidinii xylose reductase for altered cofactor specificity
journal, October 2009

  • Khoury, George A.; Fazelinia, Hossein; Chin, Jonathan W.
  • Protein Science, Vol. 18, Issue 10
  • DOI: 10.1002/pro.227

Probing the Determinants of Coenzyme Specificity in Ferredoxin-NADP + Reductase by Site-directed Mutagenesis
journal, January 2001

  • Medina, Milagros; Luquita, Alejandra; Tejero, Jesús
  • Journal of Biological Chemistry, Vol. 276, Issue 15
  • DOI: 10.1074/jbc.M009287200

Prediction of the occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprint
journal, January 1986

  • Wierenga, Rik K.; Terpstra, Peter; Hol, Wim G. J.
  • Journal of Molecular Biology, Vol. 187, Issue 1, p. 101-107
  • DOI: 10.1016/0022-2836(86)90409-2

General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH
journal, June 2013

  • Brinkmann-Chen, S.; Flock, T.; Cahn, J. K. B.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 27, p. 10946-10951
  • DOI: 10.1073/pnas.1306073110

Nucleotide sequence and characterization of a cDNA encoding the acetohydroxy acid isomeroreductase from Arabidopsis thaliana
journal, February 1993

  • Curien, Gilles; Dumas, Renaud; Douce, Roland
  • Plant Molecular Biology, Vol. 21, Issue 4
  • DOI: 10.1007/BF00014556

Purification and characterization of acetohydroxyacid reductoisomerase from spinach chloroplasts
journal, September 1989

  • Dumas, R.; Joyard, J.; Douce, R.
  • Biochemical Journal, Vol. 262, Issue 3
  • DOI: 10.1042/bj2620971

A single amino acid substitution in lactate dehydrogenase improves the catalytic efficiency with an alternative coenzyme
journal, January 1990

  • Feeney, Raymond; Clarke, Anthony R.; Holbrook, J. John
  • Biochemical and Biophysical Research Communications, Vol. 166, Issue 2, p. 667-672
  • DOI: 10.1016/0006-291X(90)90861-G