Title: Alteration and modulation of protein activity by varying post-translational modification

Abstract

Embodiments of the invention include methods of altering the enzymatic activity or solubility of an extremophilic enzyme or post-translationally modifying a protein of interest via using isolated or partially purified glycosyltransferases and/or post-translational modification proteins, extracts of cells comprising glycosyltransferases and/or post-translational modification proteins, and/or in cells comprising one or more glycosyltransferases and/or post-translational modification proteins.

Inventors:

Thompson, David N.; Reed, David W.; Thompson, Vicki S.; Lacey, Jeffrey A.; Apel, William A.

Issue Date:

2016-07-12

Research Org.:

Idaho National Lab. (INL), Idaho Falls, ID (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1261626

Patent Number(s):

9388398

Application Number:

14/632,770

Assignee:

Battelle Energy Alliance, LLC (Idaho Falls, ID)

Patent Classifications (CPCs):

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

Y02P20/52 - using catalysts, e.g. selective catalysts

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P21/005 - {Glycopeptides, glycoproteins}

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/1051 - {Hexosyltransferases
C12N9/2437 - {Cellulases
C12N9/1048 - {Glycosyltransferases

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y302/01008 - Endo-1,4-beta-xylanase

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P21/00 - Preparation of peptides or proteins

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/2477 - {Hemicellulases not provided in a preceding group}
C12N9/1288 - {Transferases for other substituted phosphate groups

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y302/01004 - Cellulase

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/2482 - {Endo-1,4-beta-xylanase
C12N9/2402 - {hydrolysing O- and S- glycosyl compounds

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DOE Contract Number:  

AC07-99ID13727; AC07-05ID14517

Resource Type:

Patent

Resource Relation:

Patent File Date: 2015 Feb 26

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES