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Title: Increasing cell culture population doublings for long-term growth of finite life span human cell cultures

Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.
Inventors:
;
Issue Date:
OSTI Identifier:
1259682
Assignee:
The Regents of the University of California (Oakland, CA) LBNL
Patent Number(s):
9,376,662
Application Number:
14/627,888
Contract Number:
AC02-05CH11231
Resource Relation:
Patent File Date: 2015 Feb 20
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

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  • DOI: 10.1073/pnas.191169098

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  • DOI: 10.1073/pnas.071483998

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