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Title: Increasing cell culture population doublings for long-term growth of finite life span human cell cultures

Abstract

Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.

Inventors:
;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1259682
Patent Number(s):
9,376,662
Application Number:
14/627,888
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Feb 20
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Stampfer, Martha R., and Garbe, James C. Increasing cell culture population doublings for long-term growth of finite life span human cell cultures. United States: N. p., 2016. Web.
Stampfer, Martha R., & Garbe, James C. Increasing cell culture population doublings for long-term growth of finite life span human cell cultures. United States.
Stampfer, Martha R., and Garbe, James C. Tue . "Increasing cell culture population doublings for long-term growth of finite life span human cell cultures". United States. https://www.osti.gov/servlets/purl/1259682.
@article{osti_1259682,
title = {Increasing cell culture population doublings for long-term growth of finite life span human cell cultures},
author = {Stampfer, Martha R. and Garbe, James C.},
abstractNote = {Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {6}
}

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Works referenced in this record:

Telomerase beyond telomeres
journal, August 2002

  • Blasco, María A.
  • Nature Reviews Cancer, Vol. 2, Issue 8
  • DOI: 10.1038/nrc862

Oxytocin and Oxytocin Receptors in Cancer Cells and Proliferation
journal, April 2004


Serum-free growth of human mammary epithelial cells: rapid clonal growth in defined medium and extended serial passage with pituitary extract.
journal, September 1984

  • Hammond, S. L.; Ham, R. G.; Stampfer, M. R.
  • Proceedings of the National Academy of Sciences, Vol. 81, Issue 17
  • DOI: 10.1073/pnas.81.17.5435

Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype
journal, January 1999

  • Jiang, Xu-Rong; Jimenez, Gretchen; Chang, Edwin
  • Nature Genetics, Vol. 21, Issue 1
  • DOI: 10.1038/5056

Telomerase protects developing neurons against DNA damage-induced cell death
journal, November 2001


Telomerase Maintains Telomere Structure in Normal Human Cells
journal, July 2003


Absence of cancer–associated changes in human fibroblasts immortalized with telomerase
journal, January 1999

  • Morales, Carmela P.; Holt, Shawn E.; Ouellette, Michel
  • Nature Genetics, Vol. 21, Issue 1
  • DOI: 10.1038/5063

Telomerase reverse transcriptase promotes cardiac muscle cell proliferation, hypertrophy, and survival
journal, August 2001

  • Oh, H.; Taffet, G. E.; Youker, K. A.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 18, p. 10308-10313
  • DOI: 10.1073/pnas.191169098

Peripherally Administered Angiotensin II AT 1 Receptor Antagonists Are Anti-stress Compounds in Vivo
journal, December 2008

  • Pavel, Jaroslav; Benicky, Julius; Murakami, Yuki
  • Annals of the New York Academy of Sciences, Vol. 1148, Issue 1
  • DOI: 10.1196/annals.1410.006

Telomerase modulates expression of growth-controlling genes and enhances cell proliferation
journal, April 2003

  • Smith, Laura L.; Coller, Hilary A.; Roberts, James M.
  • Nature Cell Biology, Vol. 5, Issue 5
  • DOI: 10.1038/ncb985

Expression of the telomerase catalytic subunit, hTERT, induces resistance to transforming growth factor β growth inhibition in p16INK4A(−) human mammary epithelial cells
journal, April 2001

  • Stampfer, M. R.; Garbe, J.; Levine, G.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 8, p. 4498-4503
  • DOI: 10.1073/pnas.071483998

Long-term culture of adult murine epidermal keratinocytes
journal, December 2005


Molecular changes accompanying senescence and immortalization of cultured human mammary epithelial cells
journal, November 2002

  • Yaswen, Paul; Stampfer, Martha R.
  • The International Journal of Biochemistry & Cell Biology, Vol. 34, Issue 11, p. 1382-1394
  • DOI: 10.1016/S1357-2725(02)00047-X