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Title: Fusion proteins useful for producing pinene

The present invention provides for a modified host cell comprising a heterologous pinene synthase (PS), or enzymatically active fragment or variant thereof, and optionally a geranyl pyrophosphate synthase (GPPS), or enzymatically active fragment or variant thereof, or a fusion protein comprising: (a) a PS and (b) a GPPS linked by a linker.
Inventors:
;
Issue Date:
OSTI Identifier:
1259654
Assignee:
The Regents of the University of California (Oakland, CA) LBNL
Patent Number(s):
9,376,691
Application Number:
14/091,818
Contract Number:
AC02-05CH11231
Resource Relation:
Patent File Date: 2013 Nov 27
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Works referenced in this record:

Metabolic engineering for advanced biofuels production from Escherichia coli
journal, October 2008

Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli
journal, November 2011
  • Bokinsky, G.; Peralta-Yahya, P. P.; George, A.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 50, p. 19949-19954
  • DOI: 10.1073/pnas.1106958108

Geranyl diphosphate synthase from Abies grandis: cDNA isolation, functional expression, and characterization
journal, September 2002

Insights into Plant Cell Wall Degradation from the Genome Sequence of the Soil Bacterium Cellvibrio japonicus
journal, June 2008
  • DeBoy, R. T.; Mongodin, E. F.; Fouts, D. E.
  • Journal of Bacteriology, Vol. 190, Issue 15, p. 5455-5463
  • DOI: 10.1128/JB.01701-07

cDNA isolation, functional expression, and characterization of (+)-α-pinene synthase and (−)-α-pinene synthase from loblolly pine (Pinus taeda): Stereocontrol in pinene biosynthesis
journal, March 2003
  • Phillips, Michael A.; Wildung, Mark R.; Williams, David C.
  • Archives of Biochemistry and Biophysics, Vol. 411, Issue 2, p. 267-276
  • DOI: 10.1016/S0003-9861(02)00746-4

Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
journal, January 2010
  • Steen, Eric J.; Kang, Yisheng; Bokinsky, Gregory
  • Nature, Vol. 463, Issue 7280, p. 559-562
  • DOI: 10.1038/nature08721