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Title: Useful halophilic, thermostable and ionic liquids tolerant cellulases

Abstract

The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.

Inventors:
; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1259651
Patent Number(s):
9376728
Application Number:
13/493,938
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Jun 11
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Zhang, Tao, Datta, Supratim, Simmons, Blake A., and Rubin, Edward M.. Useful halophilic, thermostable and ionic liquids tolerant cellulases. United States: N. p., 2016. Web.
Zhang, Tao, Datta, Supratim, Simmons, Blake A., & Rubin, Edward M.. Useful halophilic, thermostable and ionic liquids tolerant cellulases. United States.
Zhang, Tao, Datta, Supratim, Simmons, Blake A., and Rubin, Edward M.. Tue . "Useful halophilic, thermostable and ionic liquids tolerant cellulases". United States. https://www.osti.gov/servlets/purl/1259651.
@article{osti_1259651,
title = {Useful halophilic, thermostable and ionic liquids tolerant cellulases},
author = {Zhang, Tao and Datta, Supratim and Simmons, Blake A. and Rubin, Edward M.},
abstractNote = {The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {6}
}

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Works referenced in this record:

Evaluation of Three Automated Genome Annotations for Halorhabdus utahensis
journal, July 2009


Enzyme-Catalyzed Hydrolysis of Cellulose in Ionic Liquids: A Green Approach Toward the Production of Biofuels
journal, June 2010