Fluidics platform and method for sample preparation

Benner, Henry W.; Dzenitis, John M.

Title: Fluidics platform and method for sample preparation

Abstract

Provided herein are fluidics platforms and related methods for performing integrated sample collection and solid-phase extraction of a target component of the sample all in one tube. The fluidics platform comprises a pump, particles for solid-phase extraction and a particle-holding means. The method comprises contacting the sample with one or more reagents in a pump, coupling a particle-holding means to the pump and expelling the waste out of the pump while the particle-holding means retains the particles inside the pump. The fluidics platform and methods herein described allow solid-phase extraction without pipetting and centrifugation.

Inventors:: Benner, Henry W.; Dzenitis, John M.

Issue Date:: 2016-06-21

Research Org.:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1258015

Patent Number(s):: 9372135

Application Number:: 13/228,370

Assignee:: Lawrence Livermore National Security, LLC (Livermore, CA)

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

Show more

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N1/405 - {by adsorption or absorption}
G01N1/286 - {involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2400/0475 - specific mechanical means and fluid pressure

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N1/40 - Concentrating samples

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L3/502761 - {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
B01L3/50273 - {characterised by the means or forces applied to move the fluids
B01L2400/0478 - pistons
B01L2400/0605 - check valves
B01L2200/0668 - Trapping microscopic beads
B01L2200/0647 - Handling flowable solids, e.g. microscopic beads, cells, particles

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N35/0098 - {involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2200/0631 - Purification arrangements, e.g. solid phase extraction [SPE]
B01L3/5027 - {by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N1/44 - Sample treatment involving radiation, e.g. heat

Show less

DOE Contract Number:: AC52-07NA27344

Resource Type:: Patent

Resource Relation:: Patent File Date: 2011 Sep 08

Country of Publication:: United States

Language:: English

Subject:: 47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats


                    Benner, Henry W., and Dzenitis, John M. Fluidics platform and method for sample preparation.  United States: N. p., 2016. 
        Web.

Copy to clipboard


                    Benner, Henry W., & Dzenitis, John M. Fluidics platform and method for sample preparation.  United States.

Copy to clipboard


                    Benner, Henry W., and Dzenitis, John M. Tue .  
        "Fluidics platform and method for sample preparation".  United States.  https://www.osti.gov/servlets/purl/1258015.

Copy to clipboard


                    
@article{osti_1258015,

  title        = {Fluidics platform and method for sample preparation},

  author       = {Benner, Henry W. and Dzenitis, John M.},

  abstractNote = {Provided herein are fluidics platforms and related methods for performing integrated sample collection and solid-phase extraction of a target component of the sample all in one tube. The fluidics platform comprises a pump, particles for solid-phase extraction and a particle-holding means. The method comprises contacting the sample with one or more reagents in a pump, coupling a particle-holding means to the pump and expelling the waste out of the pump while the particle-holding means retains the particles inside the pump. The fluidics platform and methods herein described allow solid-phase extraction without pipetting and centrifugation.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2016},

  month        = {6}

}

Copy to clipboard

Patent:

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

An integrated portable hand-held analyser for real-time isothermal nucleic acid amplification
journal, August 2007

Smith, Matthew C.; Steimle, George; Ivanov, Stan
Analytica Chimica Acta, Vol. 598, Issue 2, p. 286-294
https://doi.org/10.1016/j.aca.2007.07.036

Similar Records in DOE Patents and OSTI.GOV collections:

Fluidics platform and method for sample preparation and analysis

Patent Benner, W. Henry; Dzenitis, John M.; Bennet, William J.; ...

Herein provided are fluidics platform and method for sample preparation and analysis. The fluidics platform is capable of analyzing DNA from blood samples using amplification assays such as polymerase-chain-reaction assays and loop-mediated-isothermal-amplification assays. The fluidics platform can also be used for other types of assays and analyzes. In some embodiments, a sample in a sealed tube can be inserted directly. The following isolation, detection, and analyzes can be performed without a user's intervention. The disclosed platform may also comprises a sample preparation system with a magnetic actuator, a heater, and an air-drying mechanism, and fluid manipulation processes for extraction, washing,more » « less
Full Text Available
Microfluidic DNA sample preparation method and device

Patent Krulevitch, Peter A [Pleasanton, CA]; Miles, Robin R [Danville, CA]; Wang, Xiao-Bo [San Diego, CA]; ...

Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizablemore » « less
Full Text Available
Method and apparatus for the preparation of liquid samples for determination of boron

Patent Siemer, Darryl D. [Idaho Falls, ID]

A method and apparatus for the preparation of a liquid sample for the quantitative determination of boron by flame photometry. The sample is combined in a vessel with sulfuric acid, and an excess of methanol is added thereto. The methanol reacts with any boron present in the sample to form trimethyl borate which is volatilized by the heat of reaction between the excess methanol and sulfuric acid. The volatilized trimethyl borate is withdrawn from the vessel by either a partial vacuum or a positive pressure and is rapidly transferred to a standard flame photometer. The method is free of interferencemore » from typical boron concomitants. « less
Full Text Available
Universal nucleic acids sample preparation method for cells, spores and their mixture

Patent Bavykin, Sergei [Darien, IL]

The present invention relates to a method for extracting nucleic acids from biological samples. More specifically the invention relates to a universal method for extracting nucleic acids from unidentified biological samples. An advantage of the presently invented method is its ability to effectively and efficiently extract nucleic acids from a variety of different cell types including but not limited to prokaryotic or eukaryotic cells and/or recalcitrant organisms (i.e. spores). Unlike prior art methods which are focused on extracting nucleic acids from vegetative cell or spores, the present invention effectively extracts nucleic acids from spores, multiple cell types or mixtures thereofmore » « less
Full Text Available
Fluidic actuator system and method

Patent Madrone, Leila Marcia; Betts, Kyle Douglas; Lynn, Peter Sturt; ...

A pneumatically actuated solar panel array system that includes a plurality of separate actuator assemblies that each have a top plate and bottom plate and a first and second bellows that each extend between and are coupled to the top and bottom plates at a respective top head and bottom head, the first and second bellows being configured to be separately pneumatically inflated, where the pneumatic inflation expands the bellows along a length. The pneumatically actuated solar panel array system can also include a plurality of solar panels coupled to the actuator assemblies with the solar panels being configured tomore » « less
Full Text Available

Similar Records

Title: Fluidics platform and method for sample preparation

Abstract

Citation Formats

An integrated portable hand-held analyser for real-time isothermal nucleic acid amplification journal, August 2007

An integrated portable hand-held analyser for real-time isothermal nucleic acid amplification
journal, August 2007