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Title: Fluidics platform and method for sample preparation

Provided herein are fluidics platforms and related methods for performing integrated sample collection and solid-phase extraction of a target component of the sample all in one tube. The fluidics platform comprises a pump, particles for solid-phase extraction and a particle-holding means. The method comprises contacting the sample with one or more reagents in a pump, coupling a particle-holding means to the pump and expelling the waste out of the pump while the particle-holding means retains the particles inside the pump. The fluidics platform and methods herein described allow solid-phase extraction without pipetting and centrifugation.
Inventors:
;
Issue Date:
OSTI Identifier:
1258015
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA) LLNL
Patent Number(s):
9,372,135
Application Number:
13/228,370
Contract Number:
AC52-07NA27344
Resource Relation:
Patent File Date: 2011 Sep 08
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Works referenced in this record:

An integrated portable hand-held analyser for real-time isothermal nucleic acid amplification
journal, August 2007
  • Smith, Matthew C.; Steimle, George; Ivanov, Stan
  • Analytica Chimica Acta, Vol. 598, Issue 2, p. 286-294
  • DOI: 10.1016/j.aca.2007.07.036