Title: Fluidics platform and method for sample preparation

Abstract

Provided herein are fluidics platforms and related methods for performing integrated sample collection and solid-phase extraction of a target component of the sample all in one tube. The fluidics platform comprises a pump, particles for solid-phase extraction and a particle-holding means. The method comprises contacting the sample with one or more reagents in a pump, coupling a particle-holding means to the pump and expelling the waste out of the pump while the particle-holding means retains the particles inside the pump. The fluidics platform and methods herein described allow solid-phase extraction without pipetting and centrifugation.

Inventors:

Benner, Henry W.; Dzenitis, John M.

Issue Date:

Tue Jun 21 00:00:00 EDT 2016

Research Org.:

Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1258015

Patent Number(s):

9372135

Application Number:

13/228,370

Assignee:

Lawrence Livermore National Security, LLC (Livermore, CA)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2200/0631 - Purification arrangements, e.g. solid phase extraction [SPE]
B01L2200/0647 - Handling flowable solids, e.g. microscopic beads, cells, particles
B01L2200/0668 - Trapping microscopic beads
B01L2400/0475 - specific mechanical means and fluid pressure
B01L2400/0478 - pistons
B01L2400/0605 - check valves
B01L3/5027 - {by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
B01L3/50273 - {characterised by the means or forces applied to move the fluids
B01L3/502761 - {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N1/286 - {involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
G01N1/40 - Concentrating samples
G01N1/405 - {by adsorption or absorption}
G01N1/44 - Sample treatment involving radiation, e.g. heat
G01N35/0098 - {involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation

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DOE Contract Number:  

AC52-07NA27344

Resource Type:

Patent

Resource Relation:

Patent File Date: 2011 Sep 08

Country of Publication:

United States

Language:

English

Subject:

47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY