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Title: Over-expression of a putative oxidoreductase (UcpA) for increasing furfural or 5-hydroxymethylfurfural tolerance

Abstract

The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED, Gainesville, FL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1254240
Patent Number(s):
9347077
Application Number:
14/131,468
Assignee:
UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED (Gainesville, FL)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG36-08GO88142
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Jul 09
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 09 BIOMASS FUELS

Citation Formats

Wang, Xuan, Miller, Elliot N., Yomano, Lorraine P., Shanmugam, Keelnatham T., and Ingram, Lonnie O'Neal. Over-expression of a putative oxidoreductase (UcpA) for increasing furfural or 5-hydroxymethylfurfural tolerance. United States: N. p., 2016. Web.
Wang, Xuan, Miller, Elliot N., Yomano, Lorraine P., Shanmugam, Keelnatham T., & Ingram, Lonnie O'Neal. Over-expression of a putative oxidoreductase (UcpA) for increasing furfural or 5-hydroxymethylfurfural tolerance. United States.
Wang, Xuan, Miller, Elliot N., Yomano, Lorraine P., Shanmugam, Keelnatham T., and Ingram, Lonnie O'Neal. Tue . "Over-expression of a putative oxidoreductase (UcpA) for increasing furfural or 5-hydroxymethylfurfural tolerance". United States. https://www.osti.gov/servlets/purl/1254240.
@article{osti_1254240,
title = {Over-expression of a putative oxidoreductase (UcpA) for increasing furfural or 5-hydroxymethylfurfural tolerance},
author = {Wang, Xuan and Miller, Elliot N. and Yomano, Lorraine P. and Shanmugam, Keelnatham T. and Ingram, Lonnie O'Neal},
abstractNote = {The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {5}
}

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