Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation

Title: Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation

Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area.

Inventors:: Schaff, Ulrich Y.; Koh, Chung-Yan; Sommer, Gregory J.

Issue Date:: 2016-04-05

OSTI Identifier:: 1245445

Assignee:: Sandia Corporation (Albuquerque, NM) SNL-A

Patent Number(s):: 9,304,129

Application Number:: 14/597,601

Contract Number:: AC04-94AL85000

Resource Relation:: Patent File Date: 2015 Jan 15

Research Org:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Org:: USDOE

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

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