Cytosolic delivery of materials with endosome-disrupting colloids
Abstract
A facile procedure to deliver nanocrystals to the cytosol of live cells that is both rapid and general. The technique employs a unique cationic core-shell polymer colloid that directs nanocrystals to the cytosol of living cells within a few hours of incubation. The present methods and compositions enable a host of advanced applications arising from efficient cytosolic delivery of nanocrystal imaging probes: from single particle tracking experiments to monitoring protein-protein interactions in live cells for extended periods.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1243071
- Patent Number(s):
- 9284188
- Application Number:
- 13/225,199
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08F - MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2011 Sep 02
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; 47 OTHER INSTRUMENTATION; 77 NANOSCIENCE AND NANOTECHNOLOGY
Citation Formats
Helms, Brett A., and Bayles, Andrea R. Cytosolic delivery of materials with endosome-disrupting colloids. United States: N. p., 2016.
Web.
Helms, Brett A., & Bayles, Andrea R. Cytosolic delivery of materials with endosome-disrupting colloids. United States.
Helms, Brett A., and Bayles, Andrea R. Tue .
"Cytosolic delivery of materials with endosome-disrupting colloids". United States. https://www.osti.gov/servlets/purl/1243071.
@article{osti_1243071,
title = {Cytosolic delivery of materials with endosome-disrupting colloids},
author = {Helms, Brett A. and Bayles, Andrea R.},
abstractNote = {A facile procedure to deliver nanocrystals to the cytosol of live cells that is both rapid and general. The technique employs a unique cationic core-shell polymer colloid that directs nanocrystals to the cytosol of living cells within a few hours of incubation. The present methods and compositions enable a host of advanced applications arising from efficient cytosolic delivery of nanocrystal imaging probes: from single particle tracking experiments to monitoring protein-protein interactions in live cells for extended periods.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {3}
}
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