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Title: Cytosolic delivery of materials with endosome-disrupting colloids

Abstract

A facile procedure to deliver nanocrystals to the cytosol of live cells that is both rapid and general. The technique employs a unique cationic core-shell polymer colloid that directs nanocrystals to the cytosol of living cells within a few hours of incubation. The present methods and compositions enable a host of advanced applications arising from efficient cytosolic delivery of nanocrystal imaging probes: from single particle tracking experiments to monitoring protein-protein interactions in live cells for extended periods.

Inventors:
;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1243071
Patent Number(s):
9284188
Application Number:
13/225,199
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Sep 02
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 47 OTHER INSTRUMENTATION; 77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats

Helms, Brett A., and Bayles, Andrea R. Cytosolic delivery of materials with endosome-disrupting colloids. United States: N. p., 2016. Web.
Helms, Brett A., & Bayles, Andrea R. Cytosolic delivery of materials with endosome-disrupting colloids. United States.
Helms, Brett A., and Bayles, Andrea R. Tue . "Cytosolic delivery of materials with endosome-disrupting colloids". United States. https://www.osti.gov/servlets/purl/1243071.
@article{osti_1243071,
title = {Cytosolic delivery of materials with endosome-disrupting colloids},
author = {Helms, Brett A. and Bayles, Andrea R.},
abstractNote = {A facile procedure to deliver nanocrystals to the cytosol of live cells that is both rapid and general. The technique employs a unique cationic core-shell polymer colloid that directs nanocrystals to the cytosol of living cells within a few hours of incubation. The present methods and compositions enable a host of advanced applications arising from efficient cytosolic delivery of nanocrystal imaging probes: from single particle tracking experiments to monitoring protein-protein interactions in live cells for extended periods.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {3}
}

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