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Title: Methods for determining enzymatic activity comprising heating and agitation of closed volumes

Abstract

Methods for determining thermophilic enzymatic activity include heating a substrate solution in a plurality of closed volumes to a predetermined reaction temperature. Without opening the closed volumes, at least one enzyme is added, substantially simultaneously, to the closed volumes. At the predetermined reaction temperature, the closed volumes are agitated and then the activity of the at least one enzyme is determined. The methods are conducive for characterizing enzymes of high-temperature reactions, with insoluble substrates, with substrates and enzymes that do not readily intermix, and with low volumes of substrate and enzyme. Systems for characterizing the enzymes are also disclosed.

Inventors:
; ; ;
Issue Date:
Research Org.:
Idaho National Lab. (INL), Idaho Falls, ID (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1243062
Patent Number(s):
9,284,596
Application Number:
14/180,161
Assignee:
Battelle Energy Alliance, LLC (Idaho Falls, ID)
DOE Contract Number:  
AC07-05ID14517
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Feb 13
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Thompson, David Neil, Henriksen, Emily DeCrescenzo, Reed, David William, and Jensen, Jill Renee. Methods for determining enzymatic activity comprising heating and agitation of closed volumes. United States: N. p., 2016. Web.
Thompson, David Neil, Henriksen, Emily DeCrescenzo, Reed, David William, & Jensen, Jill Renee. Methods for determining enzymatic activity comprising heating and agitation of closed volumes. United States.
Thompson, David Neil, Henriksen, Emily DeCrescenzo, Reed, David William, and Jensen, Jill Renee. Tue . "Methods for determining enzymatic activity comprising heating and agitation of closed volumes". United States. https://www.osti.gov/servlets/purl/1243062.
@article{osti_1243062,
title = {Methods for determining enzymatic activity comprising heating and agitation of closed volumes},
author = {Thompson, David Neil and Henriksen, Emily DeCrescenzo and Reed, David William and Jensen, Jill Renee},
abstractNote = {Methods for determining thermophilic enzymatic activity include heating a substrate solution in a plurality of closed volumes to a predetermined reaction temperature. Without opening the closed volumes, at least one enzyme is added, substantially simultaneously, to the closed volumes. At the predetermined reaction temperature, the closed volumes are agitated and then the activity of the at least one enzyme is determined. The methods are conducive for characterizing enzymes of high-temperature reactions, with insoluble substrates, with substrates and enzymes that do not readily intermix, and with low volumes of substrate and enzyme. Systems for characterizing the enzymes are also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {3}
}

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Works referenced in this record:

Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities
journal, January 2011

  • Gusakov, Alexander V.; Kondratyeva, Elena G.; Sinitsyn, Arkady P.
  • International Journal of Analytical Chemistry, Vol. 2011
  • DOI: 10.1155/2011/283658