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Title: Methods for separating particles and/or nucleic acids using isotachophoresis

According to one embodiment, a method includes co-feeding fluids comprising a leading electrolyte, a trailing electrolyte, and at least one of DNA and RNA to a channel, and applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. In another embodiment, a method includes co-feeding fluids to a channel. The fluids include a leading electrolyte, a trailing electrolyte, biological objects, at least one of DNA and RNA, and a spacer electrolyte having an electrophoretic mobility that is between an electrophoretic mobility of at least some of the biological objects and an electrophoretic mobility of the at least one of the DNA and the RNA. The method also includes applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. Other methods of isotachophoresis are disclosed in addition to these.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1243046
Assignee:
Lawrence Livermore National Security, LLC (N/A) LLNL
Patent Number(s):
9,285,340
Application Number:
12/542,215
Contract Number:
AC52-07NA27344
Resource Relation:
Patent File Date: 2009 Aug 17
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Other works cited in this record:

Miniaturised isotachophoresis of DNA
journal, July 2007
  • Prest, Jeff E.; Baldock, Sara J.; Day, Philip J. R.
  • Journal of Chromatography A, Vol. 1156, Issue 1-2, p. 154-159
  • DOI: 10.1016/j.chroma.2007.01.026

Application of monoclonal antibody techniques to biochemical research
journal, February 1981

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