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Title: Methods for separating particles and/or nucleic acids using isotachophoresis

Abstract

According to one embodiment, a method includes co-feeding fluids comprising a leading electrolyte, a trailing electrolyte, and at least one of DNA and RNA to a channel, and applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. In another embodiment, a method includes co-feeding fluids to a channel. The fluids include a leading electrolyte, a trailing electrolyte, biological objects, at least one of DNA and RNA, and a spacer electrolyte having an electrophoretic mobility that is between an electrophoretic mobility of at least some of the biological objects and an electrophoretic mobility of the at least one of the DNA and the RNA. The method also includes applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. Other methods of isotachophoresis are disclosed in addition to these.

Inventors:
; ;
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1243046
Patent Number(s):
9285340
Application Number:
12/542,215
Assignee:
Lawrence Livermore National Security, LLC (N/A)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2009 Aug 17
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Jung, Byoungsok, Ness, Kevin, and Rose, Klint A. Methods for separating particles and/or nucleic acids using isotachophoresis. United States: N. p., 2016. Web.
Jung, Byoungsok, Ness, Kevin, & Rose, Klint A. Methods for separating particles and/or nucleic acids using isotachophoresis. United States.
Jung, Byoungsok, Ness, Kevin, and Rose, Klint A. Tue . "Methods for separating particles and/or nucleic acids using isotachophoresis". United States. https://www.osti.gov/servlets/purl/1243046.
@article{osti_1243046,
title = {Methods for separating particles and/or nucleic acids using isotachophoresis},
author = {Jung, Byoungsok and Ness, Kevin and Rose, Klint A.},
abstractNote = {According to one embodiment, a method includes co-feeding fluids comprising a leading electrolyte, a trailing electrolyte, and at least one of DNA and RNA to a channel, and applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. In another embodiment, a method includes co-feeding fluids to a channel. The fluids include a leading electrolyte, a trailing electrolyte, biological objects, at least one of DNA and RNA, and a spacer electrolyte having an electrophoretic mobility that is between an electrophoretic mobility of at least some of the biological objects and an electrophoretic mobility of the at least one of the DNA and the RNA. The method also includes applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. Other methods of isotachophoresis are disclosed in addition to these.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {3}
}

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Works referenced in this record:

A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
journal, February 2007


Isotachophoresis in Free-Flow Using a Miniaturized Device
journal, June 2006


Miniaturised isotachophoresis of DNA
journal, July 2007


On-Chip Millionfold Sample Stacking Using Transient Isotachophoresis
journal, April 2006


Application of monoclonal antibody techniques to biochemical research
journal, February 1981