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Title: Butanol tolerance in microorganisms

Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. Yeast host cells provided herein comprise reduced pyruvate decarboxylase activity and modified adenylate cyclase activity. In embodiments, yeast host cells provided herein comprise resistance to butanol and increased biomass production.
Issue Date:
OSTI Identifier:
Butamax Advanced Biofuels LLC (Wilmington, DE) ARPA-E
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Resource Relation:
Patent File Date: 2013 Oct 03
Research Org:
Butamax Advanced Biofuels LLC, Wilmington, DE (United States)
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Country of Publication:
United States

Works referenced in this record:

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005
  • Chica, Roberto A.; Doucet, Nicolas; Pelletier, Joelle N.
  • Current Opinion in Biotechnology, Vol. 16, Issue 4, p. 378-384
  • DOI: 10.1016/j.copbio.2005.06.004

Practical limits of function prediction
journal, January 2000

Polypeptide Release Factors in Prokaryotes and Eukaryotes
journal, January 2002

Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001

Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007
  • Sen, S.; Venkata Dasu, V.; Mandal, B.
  • Applied Biochemistry and Biotechnology, Vol. 143, Issue 3, p. 212-223
  • DOI: 10.1007/s12010-007-8003-4

Prediction of protein function from protein sequence and structure
journal, January 1999
  • Whisstock, James C.; Lesk, Arthur M.
  • Quarterly Reviews of Biophysics, Vol. 36, Issue 3, p. 307-340
  • DOI: 10.1017/S0033583503003901

A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase
journal, November 1995
  • Wishart, Matthew J.; Denu, John M.; Williams, John A.
  • Journal of Biological Chemistry, Vol. 270, Issue 45, p. 26782-26785
  • DOI: 10.1074/jbc.270.45.26782

An Investigation of the Metabolism of Valine to Isobutyl Alcohol in Saccharomyces cerevisiae
journal, October 1998
  • Dickinson, J. Richard; Harrison, Scott J.; Hewlins, Michael J. E.
  • Journal of Biological Chemistry, Vol. 273, Issue 40, p. 25751-25756
  • DOI: 10.1074/jbc.273.40.25751

New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation
journal, June 1998
  • Dürre, P.
  • Applied Microbiology and Biotechnology, Vol. 49, Issue 6, p. 639-648
  • DOI: 10.1007/s002530051226

Technologies for butanol recovery integrated with fermentations
journal, March 1992
  • Groot, W. J.; van der Lans, R. G. J. M.; Luyben, K. Ch. A. M.
  • Process Biochemistry, Vol. 27, Issue 2, p. 61-75
  • DOI: 10.1016/0032-9592(92)80012-R

Pervaporation study on the dehydration of aqueous butanol solutions: a comparison of flux vs. permeance, separation factor vs. selectivity
journal, December 2004
  • Guo, Wei Fen; Chung, Tai-Shung; Matsuura, Takeshi
  • Journal of Membrane Science, Vol. 245, Issue 1-2, p. 199-210
  • DOI: 10.1016/j.memsci.2004.07.025

DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase
journal, December 1985

The Ras and Sch9 pathways regulate stress resistance and longevity
journal, July 2003

Codon usage tabulated from international DNA sequence databases: status for the year 2000
journal, January 2000
  • Nakamura, Yasukazu; Gojobori, Takashi; Ikemura, Toshimichi
  • Nucleic Acids Research, Vol. 28, Issue 1, p. 292-292
  • DOI: 10.1093/nar/28.1.292

Flocculation in Saccharomyces cerevisiae: a review
journal, November 2010

Molecular machinery of autophagosome formation in yeast, Saccharomyces cerevisiae
journal, March 2007

Seripauperins of Saccharomyces cerevisiae: a new multigene family encoding serine-poor relatives of serine-rich proteins
journal, October 1994

Analyses of APG13 gene involved in autophagy in yeast, Saccharomyces cerevisiae
journal, June 1997