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Title: Butanol tolerance in microorganisms

Abstract

Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. Yeast host cells provided herein comprise reduced pyruvate decarboxylase activity and modified adenylate cyclase activity. In embodiments, yeast host cells provided herein comprise resistance to butanol and increased biomass production.

Inventors:
;
Issue Date:
Research Org.:
Butamax Advanced Biofuels LLC, Wilmington, DE (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1241024
Patent Number(s):
9,273,330
Application Number:
14/045,506
Assignee:
Butamax Advanced Biofuels LLC (Wilmington, DE)
DOE Contract Number:  
AR0000006
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Oct 03
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Bramucci, Michael G., and Nagarajan, Vasantha. Butanol tolerance in microorganisms. United States: N. p., 2016. Web.
Bramucci, Michael G., & Nagarajan, Vasantha. Butanol tolerance in microorganisms. United States.
Bramucci, Michael G., and Nagarajan, Vasantha. Tue . "Butanol tolerance in microorganisms". United States. https://www.osti.gov/servlets/purl/1241024.
@article{osti_1241024,
title = {Butanol tolerance in microorganisms},
author = {Bramucci, Michael G. and Nagarajan, Vasantha},
abstractNote = {Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. Yeast host cells provided herein comprise reduced pyruvate decarboxylase activity and modified adenylate cyclase activity. In embodiments, yeast host cells provided herein comprise resistance to butanol and increased biomass production.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {3}
}

Patent:

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Works referenced in this record:

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998


Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005

  • Chica, Roberto A.; Doucet, Nicolas; Pelletier, Joelle N.
  • Current Opinion in Biotechnology, Vol. 16, Issue 4, p. 378-384
  • DOI: 10.1016/j.copbio.2005.06.004

Practical limits of function prediction
journal, January 2000


Polypeptide Release Factors in Prokaryotes and Eukaryotes
journal, January 2002


Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001


Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007

  • Sen, S.; Venkata Dasu, V.; Mandal, B.
  • Applied Biochemistry and Biotechnology, Vol. 143, Issue 3, p. 212-223
  • DOI: 10.1007/s12010-007-8003-4

Prediction of protein function from protein sequence and structure
journal, January 1999

  • Whisstock, James C.; Lesk, Arthur M.
  • Quarterly Reviews of Biophysics, Vol. 36, Issue 3, p. 307-340
  • DOI: 10.1017/S0033583503003901

A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase
journal, November 1995

  • Wishart, Matthew J.; Denu, John M.; Williams, John A.
  • Journal of Biological Chemistry, Vol. 270, Issue 45, p. 26782-26785
  • DOI: 10.1074/jbc.270.45.26782

PCR-mediated seamless gene deletion and marker recycling inSaccharomyces cerevisiae
journal, January 2006

  • Akada, Rinji; Kitagawa, Takao; Kaneko, Shohei
  • Yeast, Vol. 23, Issue 5
  • DOI: 10.1002/yea.1365

Longevity Regulation in Saccharomyces cerevisiae: Linking Metabolism, Genome Stability, and Heterochromatin
journal, September 2003


Improved sensitivity of biological sequence database searches
journal, January 1990


Ty3 integrates within the region of RNA polymerase III transcription initiation.
journal, January 1992

  • Chalker, D. L.; Sandmeyer, S. B.
  • Genes & Development, Vol. 6, Issue 1
  • DOI: 10.1101/gad.6.1.117

Ethanol production from cellulose by coupled saccharification/fermentation usingSaccharomyces cerevisiae and cellulase complex fromSclerotiun rolfsii UV-8 mutant
journal, September 1992

  • Deshpande, Mukund V.
  • Applied Biochemistry and Biotechnology, Vol. 36, Issue 3, p. 227-234
  • DOI: 10.1007/BF02921781

An Investigation of the Metabolism of Valine to Isobutyl Alcohol in Saccharomyces cerevisiae
journal, October 1998

  • Dickinson, J. Richard; Harrison, Scott J.; Hewlins, Michael J. E.
  • Journal of Biological Chemistry, Vol. 273, Issue 40, p. 25751-25756
  • DOI: 10.1074/jbc.273.40.25751

Filament formation in Saccharomyces cerevisiae — a review
journal, January 2008


New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation
journal, June 1998

  • Dürre, P.
  • Applied Microbiology and Biotechnology, Vol. 49, Issue 6, p. 639-648
  • DOI: 10.1007/s002530051226

Phosphoenolpyruvate- and ATP-Dependent Dihydroxyacetone Kinases:  Covalent Substrate-Binding and Kinetic Mechanism ,
journal, October 2004

  • Garcia-Alles, Luis F.; Siebold, Christian; Nyffeler, Therese Lüthi
  • Biochemistry, Vol. 43, Issue 41
  • DOI: 10.1021/bi048575m

Technologies for butanol recovery integrated with fermentations
journal, March 1992

  • Groot, W. J.; van der Lans, R. G. J. M.; Luyben, K. Ch. A. M.
  • Process Biochemistry, Vol. 27, Issue 2, p. 61-75
  • DOI: 10.1016/0032-9592(92)80012-R

Pervaporation study on the dehydration of aqueous butanol solutions: a comparison of flux vs. permeance, separation factor vs. selectivity
journal, December 2004

  • Guo, Wei Fen; Chung, Tai-Shung; Matsuura, Takeshi
  • Journal of Membrane Science, Vol. 245, Issue 1-2, p. 199-210
  • DOI: 10.1016/j.memsci.2004.07.025

The Cortical Protein Num1p Is Essential for Dynein-Dependent Interactions of Microtubules with the Cortex
journal, December 2000

  • Heil-Chapdelaine, Richard A.; Oberle, Jessica R.; Cooper, John A.
  • The Journal of Cell Biology, Vol. 151, Issue 6
  • DOI: 10.1083/jcb.151.6.1337

DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase
journal, December 1985


Two Glucose-sensing Pathways Converge on Rgt1 to Regulate Expression of Glucose Transporter Genes in Saccharomyces cerevisiae
journal, July 2006

  • Kim, Jeong-Ho; Johnston, Mark
  • Journal of Biological Chemistry, Vol. 281, Issue 36
  • DOI: 10.1074/jbc.M603636200

Nuclear migration in Saccbaromyces cerevisiae is controlled by the highly repetitive 313 kDa NUM1 protein
journal, November 1991

  • Kormanec, Ján; Schaaff-Gerstenschläger, Ine; Zimmermann, Friedrich K.
  • MGG Molecular & General Genetics, Vol. 230, Issue 1-2
  • DOI: 10.1007/BF00290678

Hidden Markov Models in Computational Biology
journal, February 1994

  • Krogh, Anders; Brown, Michael; Mian, I. Saira
  • Journal of Molecular Biology, Vol. 235, Issue 5
  • DOI: 10.1006/jmbi.1994.1104

Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain
journal, January 1989


The Ras and Sch9 pathways regulate stress resistance and longevity
journal, July 2003


Codon usage tabulated from international DNA sequence databases: status for the year 2000
journal, January 2000

  • Nakamura, Yasukazu; Gojobori, Takashi; Ikemura, Toshimichi
  • Nucleic Acids Research, Vol. 28, Issue 1, p. 292-292
  • DOI: 10.1093/nar/28.1.292

Glucose Signaling in Saccharomyces cerevisiae
journal, March 2006


Flocculation in Saccharomyces cerevisiae: a review
journal, November 2010


Proliferation and metabolic significance of peroxisomes in Candida boidinii during growth on d-alanine or oleic acid as the sole carbon source
journal, April 1990

  • Sulter, G. J.; Waterham, H. R.; Goodman, J. M.
  • Archives of Microbiology, Vol. 153, Issue 5
  • DOI: 10.1007/BF00248431

Molecular machinery of autophagosome formation in yeast, Saccharomyces cerevisiae
journal, March 2007


Leucine-rich repeats and carboxyl terminus are required for interaction of yeast adenylate cyclase with RAS proteins.
journal, November 1990

  • Suzuki, N.; Choe, H. R.; Nishida, Y.
  • Proceedings of the National Academy of Sciences, Vol. 87, Issue 22
  • DOI: 10.1073/pnas.87.22.8711

The use of oliodeoxynucleotide probes in chaotrope-based hybridization solutions
journal, January 1991


Seripauperins of Saccharomyces cerevisiae: a new multigene family encoding serine-poor relatives of serine-rich proteins
journal, October 1994


DNA Sequence and Mutational Analysis of Rhizobitoxine Biosynthesis Genes in Bradyrhizobium elkanii
journal, November 2001


Next-Generation DNA Sequencing Methods
journal, September 2008


Analyses of APG13 gene involved in autophagy in yeast, Saccharomyces cerevisiae
journal, June 1997


Filament formation in Saccharomyces cerevisiae — a review
journal, January 2008