Methods of producing protoporphyrin IX and bacterial mutants therefor
Abstract
The presently disclosed inventive concepts are directed in certain embodiments to a method of producing protoporphyrin IX by (1) cultivating a strain of Shewanella bacteria in a culture medium under conditions suitable for growth thereof, and (2) recovering the protoporphyrin IX from the culture medium. The strain of Shewanella bacteria comprises at least one mutant hemH gene which is incapable of normal expression, thereby causing an accumulation of protoporphyrin IX. In certain embodiments of the method, the strain of Shewanella bacteria is a strain of S. loihica, and more specifically may be S. loihica PV-4. In certain embodiments, the mutant hemH gene of the strain of Shewanella bacteria may be a mutant of shew_2229 and/or of shew_1140. In other embodiments, the presently disclosed inventive concepts are directed to mutant strains of Shewanella bacteria having at least one mutant hemH gene which is incapable of normal expression, thereby causing an accumulation of protoporphyrin IX during cultivation of the bacteria. In certain embodiments the strain of Shewanella bacteria is a strain of S. loihica, and more specifically may be S. loihica PV-4. In certain embodiments, the mutant hemH gene of the strain of Shewanella bacteria may be a mutant of shew_2229 and/ormore »
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Oklahoma, Norman, OK (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1241022
- Patent Number(s):
- 9273334
- Application Number:
- 14/349,171
- Assignee:
- The Board of Regents of the University of Oklahoma (Norman, OK)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG02-07ER64383
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2014 Mar 14
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES
Citation Formats
Zhou, Jizhong, Qiu, Dongru, He, Zhili, and Xie, Ming. Methods of producing protoporphyrin IX and bacterial mutants therefor. United States: N. p., 2016.
Web.
Zhou, Jizhong, Qiu, Dongru, He, Zhili, & Xie, Ming. Methods of producing protoporphyrin IX and bacterial mutants therefor. United States.
Zhou, Jizhong, Qiu, Dongru, He, Zhili, and Xie, Ming. Tue .
"Methods of producing protoporphyrin IX and bacterial mutants therefor". United States. https://www.osti.gov/servlets/purl/1241022.
@article{osti_1241022,
title = {Methods of producing protoporphyrin IX and bacterial mutants therefor},
author = {Zhou, Jizhong and Qiu, Dongru and He, Zhili and Xie, Ming},
abstractNote = {The presently disclosed inventive concepts are directed in certain embodiments to a method of producing protoporphyrin IX by (1) cultivating a strain of Shewanella bacteria in a culture medium under conditions suitable for growth thereof, and (2) recovering the protoporphyrin IX from the culture medium. The strain of Shewanella bacteria comprises at least one mutant hemH gene which is incapable of normal expression, thereby causing an accumulation of protoporphyrin IX. In certain embodiments of the method, the strain of Shewanella bacteria is a strain of S. loihica, and more specifically may be S. loihica PV-4. In certain embodiments, the mutant hemH gene of the strain of Shewanella bacteria may be a mutant of shew_2229 and/or of shew_1140. In other embodiments, the presently disclosed inventive concepts are directed to mutant strains of Shewanella bacteria having at least one mutant hemH gene which is incapable of normal expression, thereby causing an accumulation of protoporphyrin IX during cultivation of the bacteria. In certain embodiments the strain of Shewanella bacteria is a strain of S. loihica, and more specifically may be S. loihica PV-4. In certain embodiments, the mutant hemH gene of the strain of Shewanella bacteria may be a mutant of shew_2229 and/or shew_1140.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 01 00:00:00 EST 2016},
month = {Tue Mar 01 00:00:00 EST 2016}
}
Works referenced in this record:
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