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Title: Apparatus and method for nanoflow liquid jet and serial femtosecond x-ray protein crystallography

Techniques for nanoflow serial femtosecond x-ray protein crystallography include providing a sample fluid by mixing a plurality of a first target of interest with a carrier fluid and injecting the sample fluid into a vacuum chamber at a rate less than about 4 microliters per minute. In some embodiments, the carrier fluid has a viscosity greater than about 3 centipoise.
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Issue Date:
OSTI Identifier:
The Board of Trustees of the Leland Stanford Junior University (Palo Alto, CA) SLAC
Patent Number(s):
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Contract Number:
Resource Relation:
Patent File Date: 2013 May 16
Research Org:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org:
Country of Publication:
United States

Works referenced in this record:

Nano ES GEMMA and PDMA, new tools for the analysis of nanobioparticles—Protein complexes, lipoparticles, and viruses
journal, August 2008
  • Allmaier, Günter; Laschober, Christian; Szymanski, Wladyslaw W.
  • Journal of the American Society for Mass Spectrometry, Vol. 19, Issue 8, p. 1062-1068
  • DOI: 10.1016/j.jasms.2008.05.017

Energy-dispersive X-ray emission spectroscopy using an X-ray free-electron laser in a shot-by-shot mode
journal, November 2012
  • Alonso-Mori, R.; Kern, J.; Gildea, R. J.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 47, p. 19103-19107
  • DOI: 10.1073/pnas.1211384109

Non-destructive characterization and alignment of aerodynamically focused particle beams using single particle charge detection
journal, November 2008

Single Particle X-ray Diffractive Imaging
journal, January 2008
  • Bogan, Michael J.; Benner, W. Henry; Boutet, Sébastien
  • Nano Letters, Vol. 8, Issue 1, p. 310-316
  • DOI: 10.1021/nl072728k

Femtosecond X-ray protein nanocrystallography
journal, February 2011
  • Chapman, Henry N.; Fromme, Petra; Barty, Anton
  • Nature, Vol. 470, Issue 7332, p. 73-77
  • DOI: 10.1038/nature09750

Gas dynamic virtual nozzle for generation of microscopic droplet streams
journal, September 2008
  • DePonte, D. P.; Weierstall, U.; Schmidt, K.
  • Journal of Physics D: Applied Physics, Vol. 41, Issue 19, Article No. 195505
  • DOI: 10.1088/0022-3727/41/19/195505

SEM imaging of liquid jets
journal, June 2009

X-ray Diffraction from Membrane Protein Nanocrystals
journal, January 2011
  • Hunter, M. S.; DePonte, D. P.; Shapiro, D. A.
  • Biophysical Journal, Vol. 100, Issue 1, p. 198-206
  • DOI: 10.1016/j.bpj.2010.10.049

Room temperature femtosecond X-ray diffraction of photosystem II microcrystals
journal, June 2012
  • Kern, J.; Alonso-Mori, R.; Hellmich, J.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 25, p. 9721-9726
  • DOI: 10.1073/pnas.1204598109

Electrohydrodynamic spraying characteristics of glycerol solutions in vacuum
journal, February 2003

Electrospray-assisted characterization and deposition of chlorosomes to fabricate a biomimetic light-harvesting device
journal, January 2010
  • Modesto-Lopez, Luis B.; Thimsen, Elijah J.; Collins, Aaron M.
  • Energy Environ. Sci., Vol. 3, Issue 2, p. 216-222
  • DOI: 10.1039/B914758F

Nanoflow electrospinning serial femtosecond crystallography
journal, October 2012
  • Sierra, Raymond G.; Laksmono, Hartawan; Kern, Jan
  • Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 11, p. 1584-1587
  • DOI: 10.1107/S0907444912038152

Electrospray deposition in vacuum
journal, May 2006
  • Swarbrick, Janine C.; Taylor, J. Ben; O'Shea, James N.
  • Applied Surface Science, Vol. 252, Issue 15, p. 5622-5626
  • DOI: 10.1016/j.apsusc.2005.12.025

Shrinking droplets in electrospray ionization and their influence on chemical equilibria
journal, March 2007
  • Wortmann, Arno; Kistler-Momotova, Anna; Zenobi, Renato
  • Journal of the American Society for Mass Spectrometry, Vol. 18, Issue 3, p. 385-393
  • DOI: 10.1016/j.jasms.2006.10.010