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Title: Apparatus and method for nanoflow liquid jet and serial femtosecond x-ray protein crystallography

Abstract

Techniques for nanoflow serial femtosecond x-ray protein crystallography include providing a sample fluid by mixing a plurality of a first target of interest with a carrier fluid and injecting the sample fluid into a vacuum chamber at a rate less than about 4 microliters per minute. In some embodiments, the carrier fluid has a viscosity greater than about 3 centipoise.

Inventors:
; ;
Issue Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1240690
Patent Number(s):
9275844
Application Number:
13/896,303
Assignee:
The Board of Trustees of the Leland Stanford Junior University (Palo Alto, CA)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
DOE Contract Number:  
AC02-76SF00515
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 May 16
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Bogan, Michael J., Laksmono, Hartawan, and Sierra, Raymond G. Apparatus and method for nanoflow liquid jet and serial femtosecond x-ray protein crystallography. United States: N. p., 2016. Web.
Bogan, Michael J., Laksmono, Hartawan, & Sierra, Raymond G. Apparatus and method for nanoflow liquid jet and serial femtosecond x-ray protein crystallography. United States.
Bogan, Michael J., Laksmono, Hartawan, and Sierra, Raymond G. Tue . "Apparatus and method for nanoflow liquid jet and serial femtosecond x-ray protein crystallography". United States. https://www.osti.gov/servlets/purl/1240690.
@article{osti_1240690,
title = {Apparatus and method for nanoflow liquid jet and serial femtosecond x-ray protein crystallography},
author = {Bogan, Michael J. and Laksmono, Hartawan and Sierra, Raymond G.},
abstractNote = {Techniques for nanoflow serial femtosecond x-ray protein crystallography include providing a sample fluid by mixing a plurality of a first target of interest with a carrier fluid and injecting the sample fluid into a vacuum chamber at a rate less than about 4 microliters per minute. In some embodiments, the carrier fluid has a viscosity greater than about 3 centipoise.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {3}
}

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Works referenced in this record:

Nano ES GEMMA and PDMA, new tools for the analysis of nanobioparticles—Protein complexes, lipoparticles, and viruses
journal, August 2008


Energy-dispersive X-ray emission spectroscopy using an X-ray free-electron laser in a shot-by-shot mode
journal, November 2012


Time-resolved protein nanocrystallography using an X-ray free-electron laser
journal, January 2012


Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements
journal, December 2011


Non-destructive characterization and alignment of aerodynamically focused particle beams using single particle charge detection
journal, November 2008


Single Particle X-ray Diffractive Imaging
journal, January 2008


Femtosecond X-ray protein nanocrystallography
journal, February 2011


Gas dynamic virtual nozzle for generation of microscopic droplet streams
journal, September 2008


SEM imaging of liquid jets
journal, June 2009


Sample injection for pulsed x-ray sources
conference, May 2011


X-ray Diffraction from Membrane Protein Nanocrystals
journal, January 2011


Lipidic phase membrane protein serial femtosecond crystallography
journal, January 2012


Generation of Nonagglomerated Airborne Bacteriophage Particles Using an Electrospray Technique
journal, April 2009


Room temperature femtosecond X-ray diffraction of photosystem II microcrystals
journal, June 2012


Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature
journal, February 2013


In vivo protein crystallization opens new routes in structural biology
journal, January 2012


Electrohydrodynamic spraying characteristics of glycerol solutions in vacuum
journal, February 2003


Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser
journal, December 2011


Charge Reduction in Electrosprays:  Slender Nanojets as Intermediates
journal, March 2006


Electrospray-assisted characterization and deposition of chlorosomes to fabricate a biomimetic light-harvesting device
journal, January 2010


Electrospray deposition of carbon nanotubes in vacuum
journal, January 2007


Protein Structures under Electrospray Conditions
journal, January 2007


Powder diffraction from a continuous microjet of submicrometer protein crystals
journal, October 2008


Nanoflow electrospinning serial femtosecond crystallography
journal, October 2012


Electrospray deposition in vacuum
journal, May 2006


Shrinking droplets in electrospray ionization and their influence on chemical equilibria
journal, March 2007