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Title: RNA-guided transcriptional regulation

Abstract

Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

Inventors:
; ;
Issue Date:
Research Org.:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1239817
Patent Number(s):
9267135
Application Number:
14/319,289
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Jun 30
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Church, George M., Mali, Prashant G., and Esvelt, Kevin M. RNA-guided transcriptional regulation. United States: N. p., 2016. Web.
Church, George M., Mali, Prashant G., & Esvelt, Kevin M. RNA-guided transcriptional regulation. United States.
Church, George M., Mali, Prashant G., and Esvelt, Kevin M. Tue . "RNA-guided transcriptional regulation". United States. https://www.osti.gov/servlets/purl/1239817.
@article{osti_1239817,
title = {RNA-guided transcriptional regulation},
author = {Church, George M. and Mali, Prashant G. and Esvelt, Kevin M.},
abstractNote = {Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {2}
}

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Works referenced in this record:

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