skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Systems, devices, and methods for agglutination assays using sedimentation

Abstract

Embodiments of the present invention include methods for conducting agglutination assays using sedimentation. Aggregates may be exposed to sedimentation forces and travel through a density medium to a detection area. Microfluidic devices, such as microfluidic disks, are described for conducting the agglutination assays, as are systems for conducting the assays.

Inventors:
; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1237349
Patent Number(s):
9,244,065
Application Number:
13/423,073
Assignee:
Sandia Corporation
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Mar 16
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Schaff, Ulrich Y., Sommer, Gregory J., and Singh, Anup K. Systems, devices, and methods for agglutination assays using sedimentation. United States: N. p., 2016. Web.
Schaff, Ulrich Y., Sommer, Gregory J., & Singh, Anup K. Systems, devices, and methods for agglutination assays using sedimentation. United States.
Schaff, Ulrich Y., Sommer, Gregory J., and Singh, Anup K. Tue . "Systems, devices, and methods for agglutination assays using sedimentation". United States. https://www.osti.gov/servlets/purl/1237349.
@article{osti_1237349,
title = {Systems, devices, and methods for agglutination assays using sedimentation},
author = {Schaff, Ulrich Y. and Sommer, Gregory J. and Singh, Anup K.},
abstractNote = {Embodiments of the present invention include methods for conducting agglutination assays using sedimentation. Aggregates may be exposed to sedimentation forces and travel through a density medium to a detection area. Microfluidic devices, such as microfluidic disks, are described for conducting the agglutination assays, as are systems for conducting the assays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {1}
}

Patent:

Save / Share:

Works referenced in this record:

Infrared controlled waxes for liquid handling and storage on a CD-microfluidic platform
journal, January 2011

  • Abi-Samra, Kameel; Hanson, Ryan; Madou, Marc
  • Lab on a Chip, Vol. 11, Issue 4, p. 723-726
  • DOI: 10.1039/C0LC00160K

Cell-Free DNA—A Marker to Predict Ischemic Brain Damage in a Rat Stroke Experimental Model
journal, January 2011

  • Boyko, Matthew; Ohayon, Sharon; Goldsmith, Tomer
  • Journal of Neurosurgical Anesthesiology, Vol. 23, Issue 3, p. 222-228
  • DOI: 10.1097/ANA.0b013e31821b536a

Measurement of Circulating Cell-Free DNA Levels by a New Simple Fluorescent Test in Patients With Primary Colorectal Cancer
journal, February 2011

  • Czeiger, David; Shaked, Gad; Eini, Hadar
  • American Journal of Clinical Pathology, Vol. 135, Issue 2, p. 264-270
  • DOI: 10.1309/AJCP4RK2IHVKTTZV

A rapid direct fluorescent assay for cell-free DNA quantification in biological fluids
journal, September 2009

  • Goldshtein, H.; Hausmann, M. J; Douvdevani, A.
  • Annals of Clinical Biochemistry, Vol. 46, Issue 6, p. 488-494
  • DOI: 10.1258/acb.2009.009002

Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry
journal, January 2009

  • Holmes, David; Pettigrew, David; Reccius, Christian H.
  • Lab on a Chip, Vol. 9, Issue 20, p. 2881-2889
  • DOI: 10.1039/B910053A

A fully automated immunoassay from whole blood on a disc
journal, January 2009

  • Lee, Beom Seok; Lee, Jung-Nam; Park, Jong-Myeon
  • Lab on a Chip, Vol. 9, Issue 11, p. 1548-1555
  • DOI: 10.1039/B820321K

Bead-based microfluidic immunoassays: The next generation
journal, February 2007


Lab On A CD
journal, August 2006


Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
journal, January 2007

  • Maes, Melissa L.; Davidson, Lisa B.; McDonagh, Paul F.
  • Journal of Immunological Methods, Vol. 319, Issue 1-2, p. 79-86
  • DOI: 10.1016/j.jim.2006.10.016

Functional integration of DNA purification and concentration into a real time micro-PCR chip
journal, January 2011

  • Min, Junhong; Kim, Joon-Ho; Lee, Youngsun
  • Lab on a Chip, Vol. 11, Issue 2, p. 259-265
  • DOI: 10.1039/C0LC00320D

Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients
journal, April 2006

  • Rhodes, Andrew; Wort, Stephen J.; Thomas, Helen
  • Critical Care, Vol. 10, Issue 2, Article No. R60
  • DOI: 10.1186/cc4894

A B Cell-Based Sensor for Rapid Identification of Pathogens
journal, July 2003

  • Rider, Todd H.; Petrovick, Martha S.; Nargi, Frances E.
  • Science, Vol. 301, Issue 5630, p. 213-215
  • DOI: 10.1126/science.1084920

Read-out concepts for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms
journal, February 2006

  • Riegger, L.; Grumann, M.; Nann, T.
  • Sensors and Actuators A: Physical, Vol. 126, Issue 2, p. 455-462
  • DOI: 10.1016/j.sna.2005.11.006

Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation
journal, March 2011


A new biodosimetric method: branched DNA-based quantitative detection of B1 DNA in mouse plasma
journal, August 2010

  • Zhang, L.; Zhang, M.; Yang, S.
  • The British Journal of Radiology, Vol. 83, Issue 992, p. 694-701
  • DOI: 10.1259/bjr/49886569

Circulating DNA: a new diagnostic gold mine?
journal, October 2002

  • Ziegler, Annemarie; Zangemeister-Wittke, Uwe; Stahel, Rolf A.
  • Cancer Treatment Reviews, Vol. 28, Issue 5, p. 255-271
  • DOI: 10.1016/S0305-7372(02)00077-4

How Hofmeister ion interactions affect protein stability
journal, October 1996


A molecular approach to bioseparations: Protein–protein and protein–salt interactions
journal, February 2006


Foil Air/Gas Bearing Technology — An Overview
conference, June 1997

  • Agrawal, Giri L.
  • ASME 1997 International Gas Turbine and Aeroengine Congress and Exhibition
  • DOI: 10.1115/97-GT-347

Staphylococcal Enterotoxin B as a Biological Weapon: Recognition, Management, and Surveillance of Staphylococcal Enterotoxin
journal, September 2006

  • Ahanotu, Ejem; Alvelo-Ceron, Damaris; Ravita, Timothy
  • Applied Biosafety, Vol. 11, Issue 3, p. 120-126
  • DOI: 10.1177/153567600601100303

Micro freef-low IEF enhanced by active cooling and functionalized gels
journal, December 2006

  • Albrecht, Jacob W.; Jensen, Klavs F.
  • ELECTROPHORESIS, Vol. 27, Issue 24, p. 4960-4969
  • DOI: 10.1002/elps.200600436

Ricin A-Chain Activity on Stem−Loop and Unstructured DNA Substrates
journal, March 2005

  • Amukele, Tim K.; Roday, Setu; Schramm, Vern L.
  • Biochemistry, Vol. 44, Issue 11, p. 4416-4425
  • DOI: 10.1021/bi0474362

One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST)
journal, January 2011

  • Berry, Scott M.; Alarid, Elaine T.; Beebe, David J.
  • Lab on a Chip, Vol. 11, Issue 10, p. 1747-1753
  • DOI: 10.1039/C1LC00004G

Shiga toxin 1 acting on DNA in vitro is a heat-stable enzyme not requiring proteolytic activation
journal, April 2004

  • Brigotti, Maurizio; Carnicelli, Domenica; Vara, Antonio González
  • Biochimie, Vol. 86, Issue 4-5, p. 305-309
  • DOI: 10.1016/j.biochi.2004.03.009

Formation of Natural pH Gradients in a Microfluidic Device under Flow Conditions:  Model and Experimental Validation
journal, February 2001

  • Cabrera, Catherine R.; Finlayson, Bruce; Yager, Paul
  • Analytical Chemistry, Vol. 73, Issue 3, p. 658-666
  • DOI: 10.1021/ac000495a

Rapid detection of a specific trimethoprim resistance gene using a biotinylated DNA probe
journal, January 1987

  • Carter, G. I.; Towner, K. J.; Slack, R. C. B.
  • Journal of Antimicrobial Chemotherapy, Vol. 20, Issue 3
  • DOI: 10.1093/jac/20.3.335

Multistage Isoelectric Focusing in a Polymeric Microfluidic Chip
journal, December 2005

  • Cui, Huanchun; Horiuchi, Keisuke; Dutta, Prashanta
  • Analytical Chemistry, Vol. 77, Issue 24, p. 7878-7886
  • DOI: 10.1021/ac050781s

Effects of separation length and voltage on isoelectric focusing in a plastic microfluidic device
journal, September 2006


Detecting Single Stranded DNA with a Solid State Nanopore
journal, October 2005

  • Fologea, Daniel; Gershow, Marc; Ledden, Bradley
  • Nano Letters, Vol. 5, Issue 10, p. 1905-1909
  • DOI: 10.1021/nl051199m

A Review of the State-of-the-Art for the Design of Self-Acting Gas-Lubricated Bearings
journal, January 1969

  • Fuller, D. D.
  • Journal of Lubrication Technology, Vol. 91, Issue 1
  • DOI: 10.1115/1.3554857

The current state of two-dimensional electrophoresis with immobilized pH gradients
journal, April 2000


Capillary columns with in situ formed porous monolithic packing for micro high-performance liquid chromatography and capillary electrochromatography
journal, September 1999


Integrated Preconcentration SDS−PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels
journal, July 2006

  • Hatch, Anson V.; Herr, Amy E.; Throckmorton, Daniel J.
  • Analytical Chemistry, Vol. 78, Issue 14, p. 4976-4984
  • DOI: 10.1021/ac0600454

Microfluidic immunoassays as rapid saliva-based clinical diagnostics
journal, March 2007

  • Herr, A. E.; Hatch, A. V.; Throckmorton, D. J.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 13, p. 5268-5273
  • DOI: 10.1073/pnas.0607254104

On-Chip Coupling of Isoelectric Focusing and Free Solution Electrophoresis for Multidimensional Separations
journal, March 2003

  • Herr, Amy E.; Molho, Joshua I.; Drouvalakis, Katerina A.
  • Analytical Chemistry, Vol. 75, Issue 5, p. 1180-1187
  • DOI: 10.1021/ac026239a

Depurination of A4256 in 28 S rRNA by the Ribosome-inactivating Proteins from Barley and Ricin Results in Different Ribosome Conformations
journal, May 1996

  • Holmberg, Lovisa; Nygård, Odd
  • Journal of Molecular Biology, Vol. 259, Issue 1, p. 81-94
  • DOI: 10.1006/jmbi.1996.0303

Microfabrication of a tapered channel for isoelectric focusing with thermally generated pH gradient
journal, October 2002


Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood
journal, January 2011

  • Lee, Beom Seok; Lee, Yang Ui; Kim, Han-Sang
  • Lab on a Chip, Vol. 11, Issue 1, p. 70-78
  • DOI: 10.1039/C0LC00205D

Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer
journal, June 2007

  • Lim, Peniel; North, Robert; Vigh, Gyula
  • ELECTROPHORESIS, Vol. 28, Issue 12, p. 1851-1859
  • DOI: 10.1002/elps.200600846

Photopolymerized diffusion-defined polyacrylamide gradient gels for on-chip protein sizing
journal, January 2008

  • Lo, Catherine T.; Throckmorton, Daniel J.; Singh, Anup K.
  • Lab on a Chip, Vol. 8, Issue 8, p. 1273-1279
  • DOI: 10.1039/B804485F

Integration of nanoporous membranes for sample filtration/preconcentration in microchip electrophoresis
journal, December 2006

  • Long, Zhicheng; Liu, Dayu; Ye, Nannan
  • ELECTROPHORESIS, Vol. 27, Issue 24, p. 4927-4934
  • DOI: 10.1002/elps.200600252

Preparative-scale isoelectric trapping separations using a modified Gradiflow unit
journal, December 2002


Molecular Detection of Bacterial Pathogens Using Microparticle Enhanced Double-Stranded DNA Probes
journal, August 2011

  • Riahi, Reza; Mach, Kathleen E.; Mohan, Ruchika
  • Analytical Chemistry, Vol. 83, Issue 16, p. 6349-6354
  • DOI: 10.1021/ac2012575

The Alpher, Bethe and Gamow of IEF, the alpha-Centaury of electrokinetic methodologies. Part II: Immobilized pH gradients
journal, February 2007


The Alpher, Bethe, Gamow of isoelectric focusing, the alpha-Centaury of electrokinetic methodologies. Part I
journal, March 2006


Design Optimization of Spirally Grooved Thrust Air Bearings for Polygon Mirror Laser Scanners
journal, January 1993

  • Satomi, Tadaatsu; Lin, Gang
  • JSME international journal. Ser. C, Dynamics, control, robotics, design and manufacturing, Vol. 36, Issue 3
  • DOI: 10.1299/jsmec1993.36.393

On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
journal, May 2008

  • Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V.
  • Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333
  • DOI: 10.1021/ac702523g

Miniaturized capillary isoelectric focusing in plastic microfluidic devices
journal, October 2002


Characterization of EBV-genome negative “null” and “T” cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma
journal, May 1977

  • Schneider, Ulrich; Schwenk, Hans-Ulrich; Bornkamm, Georg
  • International Journal of Cancer, Vol. 19, Issue 5, p. 621-626
  • DOI: 10.1002/ijc.2910190505

Theory of the Air-Supported Puck
journal, April 1964

  • Whitney, W. M.
  • American Journal of Physics, Vol. 32, Issue 4
  • DOI: 10.1119/1.1970264

Bioinformatic processing to identify single nucleotide polymorphism that potentially affect Ape1 function
journal, June 2011

  • Yu, Eizadora T.; Hadi, Masood Z.
  • Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 722, Issue 2, p. 140-146
  • DOI: 10.1016/j.mrgentox.2010.06.015

Parallel isoelectric focusing chip
journal, September 2004

  • Zilberstein, Gleb; Korol, Leonid; Bukshpan, Shmuel
  • PROTEOMICS, Vol. 4, Issue 9, p. 2533-2540
  • DOI: 10.1002/pmic.200300794

Parallel isoelectric focusing II
journal, November 2004

  • Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel
  • ELECTROPHORESIS, Vol. 25, Issue 21-22, p. 3643-3651
  • DOI: 10.1002/elps.200406117

Parallel processing in the isoelectric focusing chip
journal, November 2003

  • Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel
  • ELECTROPHORESIS, Vol. 24, Issue 21, p. 3735-3744
  • DOI: 10.1002/elps.200305663

Parallel Nanoliter Microfluidic Analysis System
journal, June 2007

  • Andersson, Per; Jesson, Gerald; Kylberg, Gunnar
  • Analytical Chemistry, Vol. 79, Issue 11, p. 4022-4030
  • DOI: 10.1021/ac061692y

Cell-Free Plasma DNA as a Predictor of Outcome in Severe Sepsis and Septic Shock
journal, April 2008


Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection
journal, January 2007

  • McBain, S. C.; Yiu, H. H. P.; El Haj, A.
  • Journal of Materials Chemistry, Vol. 17, Issue 24, p. 2561-2565
  • DOI: 10.1039/B617402G

Molecular Detection of Bacterial Pathogens Using Microparticle Enhanced Double-Stranded DNA Probes
journal, August 2011

  • Riahi, Reza; Mach, Kathleen E.; Mohan, Ruchika
  • Analytical Chemistry, Vol. 83, Issue 16, p. 6349-6354
  • DOI: 10.1021/ac2012575

Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates
journal, December 2003

  • Berlier, Judith E.; Rothe, Anca; Buller, Gayle
  • Journal of Histochemistry & Cytochemistry, Vol. 51, Issue 12, p. 1699-1712
  • DOI: 10.1177/002215540305101214