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Title: Systems, devices, and methods for agglutination assays using sedimentation

Embodiments of the present invention include methods for conducting agglutination assays using sedimentation. Aggregates may be exposed to sedimentation forces and travel through a density medium to a detection area. Microfluidic devices, such as microfluidic disks, are described for conducting the agglutination assays, as are systems for conducting the assays.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1237349
Assignee:
Sandia Corporation SNL-A
Patent Number(s):
9,244,065
Application Number:
13/423,073
Contract Number:
AC04-94AL85000
Resource Relation:
Patent File Date: 2012 Mar 16
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Works referenced in this record:

Infrared controlled waxes for liquid handling and storage on a CD-microfluidic platform
journal, January 2011
  • Abi-Samra, Kameel; Hanson, Ryan; Madou, Marc
  • Lab on a Chip, Vol. 11, Issue 4, p. 723-726
  • DOI: 10.1039/C0LC00160K

Cell-Free DNA—A Marker to Predict Ischemic Brain Damage in a Rat Stroke Experimental Model
journal, January 2011
  • Boyko, Matthew; Ohayon, Sharon; Goldsmith, Tomer
  • Journal of Neurosurgical Anesthesiology, Vol. 23, Issue 3, p. 222-228
  • DOI: 10.1097/ANA.0b013e31821b536a

Measurement of Circulating Cell-Free DNA Levels by a New Simple Fluorescent Test in Patients With Primary Colorectal Cancer
journal, February 2011
  • Czeiger, David; Shaked, Gad; Eini, Hadar
  • American Journal of Clinical Pathology, Vol. 135, Issue 2, p. 264-270
  • DOI: 10.1309/AJCP4RK2IHVKTTZV

Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry
journal, January 2009
  • Holmes, David; Pettigrew, David; Reccius, Christian H.
  • Lab on a Chip, Vol. 9, Issue 20, p. 2881-2889
  • DOI: 10.1039/B910053A

A fully automated immunoassay from whole blood on a disc
journal, January 2009
  • Lee, Beom Seok; Lee, Jung-Nam; Park, Jong-Myeon
  • Lab on a Chip, Vol. 9, Issue 11, p. 1548-1555
  • DOI: 10.1039/B820321K

Bead-based microfluidic immunoassays: The next generation
journal, February 2007

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
journal, January 2007
  • Maes, Melissa L.; Davidson, Lisa B.; McDonagh, Paul F.
  • Journal of Immunological Methods, Vol. 319, Issue 1-2, p. 79-86
  • DOI: 10.1016/j.jim.2006.10.016

Functional integration of DNA purification and concentration into a real time micro-PCR chip
journal, January 2011
  • Min, Junhong; Kim, Joon-Ho; Lee, Youngsun
  • Lab on a Chip, Vol. 11, Issue 2, p. 259-265
  • DOI: 10.1039/C0LC00320D

Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients
journal, April 2006
  • Rhodes, Andrew; Wort, Stephen J.; Thomas, Helen
  • Critical Care, Vol. 10, Issue 2, Article No. R60
  • DOI: 10.1186/cc4894

A B Cell-Based Sensor for Rapid Identification of Pathogens
journal, July 2003
  • Rider, Todd H.; Petrovick, Martha S.; Nargi, Frances E.
  • Science, Vol. 301, Issue 5630, p. 213-215
  • DOI: 10.1126/science.1084920

Read-out concepts for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms
journal, February 2006
  • Riegger, L.; Grumann, M.; Nann, T.
  • Sensors and Actuators A: Physical, Vol. 126, Issue 2, p. 455-462
  • DOI: 10.1016/j.sna.2005.11.006

Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation
journal, March 2011

A new biodosimetric method: branched DNA-based quantitative detection of B1 DNA in mouse plasma
journal, August 2010
  • Zhang, L.; Zhang, M.; Yang, S.
  • The British Journal of Radiology, Vol. 83, Issue 992, p. 694-701
  • DOI: 10.1259/bjr/49886569

Circulating DNA: a new diagnostic gold mine?
journal, October 2002
  • Ziegler, Annemarie; Zangemeister-Wittke, Uwe; Stahel, Rolf A.
  • Cancer Treatment Reviews, Vol. 28, Issue 5, p. 255-271
  • DOI: 10.1016/S0305-7372(02)00077-4

How Hofmeister ion interactions affect protein stability
journal, October 1996

A molecular approach to bioseparations: Protein–protein and protein–salt interactions
journal, February 2006

Micro freef-low IEF enhanced by active cooling and functionalized gels
journal, December 2006
  • Albrecht, Jacob W.; Jensen, Klavs F.
  • ELECTROPHORESIS, Vol. 27, Issue 24, p. 4960-4969
  • DOI: 10.1002/elps.200600436

Ricin A-Chain Activity on Stem−Loop and Unstructured DNA Substrates
journal, March 2005
  • Amukele, Tim K.; Roday, Setu; Schramm, Vern L.
  • Biochemistry, Vol. 44, Issue 11, p. 4416-4425
  • DOI: 10.1021/bi0474362

One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST)
journal, January 2011
  • Berry, Scott M.; Alarid, Elaine T.; Beebe, David J.
  • Lab on a Chip, Vol. 11, Issue 10, p. 1747-1753
  • DOI: 10.1039/C1LC00004G

Shiga toxin 1 acting on DNA in vitro is a heat-stable enzyme not requiring proteolytic activation
journal, April 2004
  • Brigotti, Maurizio; Carnicelli, Domenica; Vara, Antonio González
  • Biochimie, Vol. 86, Issue 4-5, p. 305-309
  • DOI: 10.1016/j.biochi.2004.03.009

Formation of Natural pH Gradients in a Microfluidic Device under Flow Conditions:  Model and Experimental Validation
journal, February 2001
  • Cabrera, Catherine R.; Finlayson, Bruce; Yager, Paul
  • Analytical Chemistry, Vol. 73, Issue 3, p. 658-666
  • DOI: 10.1021/ac000495a

Multistage Isoelectric Focusing in a Polymeric Microfluidic Chip
journal, December 2005
  • Cui, Huanchun; Horiuchi, Keisuke; Dutta, Prashanta
  • Analytical Chemistry, Vol. 77, Issue 24, p. 7878-7886
  • DOI: 10.1021/ac050781s

Effects of separation length and voltage on isoelectric focusing in a plastic microfluidic device
journal, September 2006

Detecting Single Stranded DNA with a Solid State Nanopore
journal, October 2005
  • Fologea, Daniel; Gershow, Marc; Ledden, Bradley
  • Nano Letters, Vol. 5, Issue 10, p. 1905-1909
  • DOI: 10.1021/nl051199m

The current state of two-dimensional electrophoresis with immobilized pH gradients
journal, April 2000

Capillary columns with in situ formed porous monolithic packing for micro high-performance liquid chromatography and capillary electrochromatography
journal, September 1999

Integrated Preconcentration SDS−PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels
journal, July 2006
  • Hatch, Anson V.; Herr, Amy E.; Throckmorton, Daniel J.
  • Analytical Chemistry, Vol. 78, Issue 14, p. 4976-4984
  • DOI: 10.1021/ac0600454

Microfluidic immunoassays as rapid saliva-based clinical diagnostics
journal, March 2007
  • Herr, A. E.; Hatch, A. V.; Throckmorton, D. J.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 13, p. 5268-5273
  • DOI: 10.1073/pnas.0607254104

On-Chip Coupling of Isoelectric Focusing and Free Solution Electrophoresis for Multidimensional Separations
journal, March 2003
  • Herr, Amy E.; Molho, Joshua I.; Drouvalakis, Katerina A.
  • Analytical Chemistry, Vol. 75, Issue 5, p. 1180-1187
  • DOI: 10.1021/ac026239a

Depurination of A4256 in 28 S rRNA by the Ribosome-inactivating Proteins from Barley and Ricin Results in Different Ribosome Conformations
journal, May 1996
  • Holmberg, Lovisa; Nygård, Odd
  • Journal of Molecular Biology, Vol. 259, Issue 1, p. 81-94
  • DOI: 10.1006/jmbi.1996.0303

Microfabrication of a tapered channel for isoelectric focusing with thermally generated pH gradient
journal, October 2002

Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood
journal, January 2011
  • Lee, Beom Seok; Lee, Yang Ui; Kim, Han-Sang
  • Lab on a Chip, Vol. 11, Issue 1, p. 70-78
  • DOI: 10.1039/C0LC00205D

Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer
journal, June 2007
  • Lim, Peniel; North, Robert; Vigh, Gyula
  • ELECTROPHORESIS, Vol. 28, Issue 12, p. 1851-1859
  • DOI: 10.1002/elps.200600846

Photopolymerized diffusion-defined polyacrylamide gradient gels for on-chip protein sizing
journal, January 2008
  • Lo, Catherine T.; Throckmorton, Daniel J.; Singh, Anup K.
  • Lab on a Chip, Vol. 8, Issue 8, p. 1273-1279
  • DOI: 10.1039/B804485F

Integration of nanoporous membranes for sample filtration/preconcentration in microchip electrophoresis
journal, December 2006
  • Long, Zhicheng; Liu, Dayu; Ye, Nannan
  • ELECTROPHORESIS, Vol. 27, Issue 24, p. 4927-4934
  • DOI: 10.1002/elps.200600252

Preparative-scale isoelectric trapping separations using a modified Gradiflow unit
journal, December 2002

The Alpher, Bethe and Gamow of IEF, the alpha-Centaury of electrokinetic methodologies. Part II: Immobilized pH gradients
journal, February 2007

The Alpher, Bethe, Gamow of isoelectric focusing, the alpha-Centaury of electrokinetic methodologies. Part I
journal, March 2006

On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
journal, May 2008
  • Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V.
  • Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333
  • DOI: 10.1021/ac702523g

Miniaturized capillary isoelectric focusing in plastic microfluidic devices
journal, October 2002

Characterization of EBV-genome negative “null” and “T” cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma
journal, May 1977
  • Schneider, Ulrich; Schwenk, Hans-Ulrich; Bornkamm, Georg
  • International Journal of Cancer, Vol. 19, Issue 5, p. 621-626
  • DOI: 10.1002/ijc.2910190505

Bioinformatic processing to identify single nucleotide polymorphism that potentially affect Ape1 function
journal, June 2011
  • Yu, Eizadora T.; Hadi, Masood Z.
  • Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 722, Issue 2, p. 140-146
  • DOI: 10.1016/j.mrgentox.2010.06.015

Parallel isoelectric focusing chip
journal, September 2004
  • Zilberstein, Gleb; Korol, Leonid; Bukshpan, Shmuel
  • PROTEOMICS, Vol. 4, Issue 9, p. 2533-2540
  • DOI: 10.1002/pmic.200300794

Parallel isoelectric focusing II
journal, November 2004
  • Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel
  • ELECTROPHORESIS, Vol. 25, Issue 21-22, p. 3643-3651
  • DOI: 10.1002/elps.200406117

Parallel processing in the isoelectric focusing chip
journal, November 2003
  • Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel
  • ELECTROPHORESIS, Vol. 24, Issue 21, p. 3735-3744
  • DOI: 10.1002/elps.200305663

Parallel Nanoliter Microfluidic Analysis System
journal, June 2007
  • Andersson, Per; Jesson, Gerald; Kylberg, Gunnar
  • Analytical Chemistry, Vol. 79, Issue 11, p. 4022-4030
  • DOI: 10.1021/ac061692y

Cell-Free Plasma DNA as a Predictor of Outcome in Severe Sepsis and Septic Shock
journal, April 2008

Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection
journal, January 2007
  • McBain, S. C.; Yiu, H. H. P.; El Haj, A.
  • Journal of Materials Chemistry, Vol. 17, Issue 24, p. 2561-2565
  • DOI: 10.1039/B617402G