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Title: Hydrogenase polypeptide and methods of use

Abstract

Provided herein are polypeptides having hydrogenase activity. The polypeptide may be multimeric, and may have hydrogenase activity of at least 0.05 micromoles H.sub.2 produced min.sup.-1 mg protein.sup.-1. Also provided herein are polynucleotides encoding the polypeptides, genetically modified microbes that include polynucleotides encoding one or more subunits of the multimeric polypeptide, and methods for making and using the polypeptides.

Inventors:
; ; ;
Issue Date:
Research Org.:
University of Georgia Research Foundation, Inc., Athens, GA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1237279
Patent Number(s):
9,249,440
Application Number:
14/204,802
Assignee:
University of Georgia Research Foundation, Inc.
DOE Contract Number:  
FG02-05ER15710
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Mar 11
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 08 HYDROGEN

Citation Formats

Adams, Michael W.W., Hopkins, Robert C., Jenney, JR, Francis E., and Sun, Junsong. Hydrogenase polypeptide and methods of use. United States: N. p., 2016. Web.
Adams, Michael W.W., Hopkins, Robert C., Jenney, JR, Francis E., & Sun, Junsong. Hydrogenase polypeptide and methods of use. United States.
Adams, Michael W.W., Hopkins, Robert C., Jenney, JR, Francis E., and Sun, Junsong. Tue . "Hydrogenase polypeptide and methods of use". United States. https://www.osti.gov/servlets/purl/1237279.
@article{osti_1237279,
title = {Hydrogenase polypeptide and methods of use},
author = {Adams, Michael W.W. and Hopkins, Robert C. and Jenney, JR, Francis E. and Sun, Junsong},
abstractNote = {Provided herein are polypeptides having hydrogenase activity. The polypeptide may be multimeric, and may have hydrogenase activity of at least 0.05 micromoles H.sub.2 produced min.sup.-1 mg protein.sup.-1. Also provided herein are polynucleotides encoding the polypeptides, genetically modified microbes that include polynucleotides encoding one or more subunits of the multimeric polypeptide, and methods for making and using the polypeptides.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {2}
}

Patent:

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Works referenced in this record:

Cloning and Expression inEscherichia coliof the Recombinant His-Tagged DNA Polymerases fromPyrococcus furiosusandPyrococcus woesei
journal, October 1998

  • Dąbrowski, Sławomir; Kur, Józef
  • Protein Expression and Purification, Vol. 14, Issue 1
  • DOI: 10.1006/prep.1998.0945

General vectors for archaeal hyperthermophiles: Strategies based on a mobile intron and a plasmid
journal, May 1996


Hydrogenase
journal, December 1980

  • Adams, Michael W. W.; Mortenson, Leonard E.; Chen, Jiann-Shin
  • Biochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, Vol. 594, Issue 2-3, p. 105-176
  • DOI: 10.1016/0304-4173(80)90007-5

The structure and mechanism of iron-hydrogenases
journal, November 1990

  • Adams, Michael W. W.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1020, Issue 2, p. 115-145
  • DOI: 10.1016/0005-2728(90)90044-5

Nickel hydrogenases: in search of the active site
journal, December 1994

  • Albracht, Simon P. J.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1188, Issue 3, p. 167-204
  • DOI: 10.1016/0005-2728(94)90036-1

Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins
journal, July 2006

  • Arnau, José; Lauritzen, Conni; Petersen, Gitte E.
  • Protein Expression and Purification, Vol. 48, Issue 1, p. 1-13
  • DOI: 10.1016/j.pep.2005.12.002

Infrared-Detectable Group Senses Changes in Charge Density on the Nickel Center in Hydrogenase from Chromatium vinosum
journal, April 1995

  • Bagley, Kimberly A.; Duin, Evert C.; Roseboom, W.
  • Biochemistry, Vol. 34, Issue 16
  • DOI: 10.1021/bi00016a026

Generation of New Hydrogen-Recycling Rhizobiaceae Strains by Introduction of a Novel hup Minitransposon
journal, October 2000


Maturation of [NiFe]-hydrogenases in Escherichia coli: The HypC Cycle
journal, November 2002


Catalytic partial oxidation of natural gas to syngas
journal, April 1995


Maturation of Hydrogenases
book, September 2006


Deciphering the message in protein sequences: tolerance to amino acid substitutions
journal, March 1990


[Fe 2 (SR) 2 (μ-CO)(CNMe) 6 ] 2+ and Analogues:  A New Class of Diiron Dithiolates as Structural Models for the H o x A ir State of the Fe-Only Hydrogenase
journal, November 2004

  • Boyke, Christine A.; Rauchfuss, Thomas B.; Wilson, Scott R.
  • Journal of the American Chemical Society, Vol. 126, Issue 46
  • DOI: 10.1021/ja049050k

Thermotolerant Hydrogenases: Biological Diversity, Properties, and Biotechnological Applications
journal, January 2008


Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100°C
journal, June 1986

  • Fiala, Gerhard; Stetter, Karl O.
  • Archives of Microbiology, Vol. 145, Issue 1, p. 56-61
  • DOI: 10.1007/BF00413027

Molecular Biology of Hydrogen Utilization in Aerobic Chemolithotrophs
journal, October 1993


Structural bases for the catalytic mechanism of [NiFe] hydrogenases
journal, August 1998

  • Garcin, E.; Montet, Y.; Volbeda, A.
  • Biochemical Society Transactions, Vol. 26, Issue 3
  • DOI: 10.1042/bst0260396

Review of fuel processing catalysts for hydrogen production in PEM fuel cell systems
journal, October 2002


Biological activition of hydrogen
journal, January 1997

  • Happe, Randolph P.; Roseboom, Winfried; Pierik, Antonio J.
  • Nature, Vol. 385, Issue 6612
  • DOI: 10.1038/385126a0

Large-scale applications of NAD(P)-dependent oxidoreductases: recent developments
journal, December 1999


Stability and sulfur-reduction activity in non-aqueous phase liquids of the hydrogenase from the hyperthermophile Pyrococcus furiosus
journal, October 1999


Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System
journal, March 2006


Requirements for Heterologous Production of a Complex Metalloenzyme: the Membrane-Bound [NiFe] Hydrogenase
journal, September 2005


[18] Hydrogenases I and II from Pyrococcus furiosus
book, January 2001


Characterization of Hydrogenase II from the Hyperthermophilic Archaeon Pyrococcus furiosus and Assessment of Its Role in Sulfur Reduction
journal, April 2000


Hydrogen production from pyruvate by enzymes purified from the hyperthermophilic archaeon, Pyrococcus furiosus : A key role for NADPH
journal, October 1994


Enhanced hydrogen production from glucose by metabolically engineered Escherichia coli
journal, October 2007

  • Maeda, Toshinari; Sanchez-Torres, Viviana; Wood, Thomas K.
  • Applied Microbiology and Biotechnology, Vol. 77, Issue 4
  • DOI: 10.1007/s00253-007-1217-0

Inhibition of hydrogen uptake in Escherichia coli by expressing the hydrogenase from the cyanobacterium Synechocystis sp. PCC 6803
journal, January 2007

  • Maeda, Toshinari; Vardar, Gönül; Self, William T.
  • BMC Biotechnology, Vol. 7, Issue 1
  • DOI: 10.1186/1472-6750-7-25

Escherichia coli hydrogenase 3 is a reversible enzyme possessing hydrogen uptake and synthesis activities
journal, August 2007

  • Maeda, Toshinari; Sanchez-Torres, Viviana; Wood, Thomas K.
  • Applied Microbiology and Biotechnology, Vol. 76, Issue 5
  • DOI: 10.1007/s00253-007-1086-6

Practical applications of hydrogenase I from Pyrococcus furiosus for NADPH generation and regeneration
journal, October 2003

  • Mertens, Rita; Greiner, Lasse; van den Ban, Eyke C. D.
  • Journal of Molecular Catalysis B: Enzymatic, Vol. 24-25, p. 39-52
  • DOI: 10.1016/S1381-1177(03)00071-7

[FeFe] hydrogenases and their evolution: a genomic perspective
journal, March 2007


Molecular cloning, characterization, and overexpression of a novel [Fe]-hydrogenase isolated from a high rate of hydrogen producing Enterobacter cloacae IIT-BT 08
journal, November 2004

  • Mishra, Jayshree; Khurana, Seema; Kumar, Narendra
  • Biochemical and Biophysical Research Communications, Vol. 324, Issue 2, p. 679-685
  • DOI: 10.1016/j.bbrc.2004.09.108

Overexpression of a hydrogenase gene in Clostridium paraputrificum to enhance hydrogen gas production
journal, May 2005


Fe-only hydrogenases: structure, function and evolution
journal, July 2002

  • Nicolet, Yvain; Cavazza, Christine; Fontecilla-Camps, J. C.
  • Journal of Inorganic Biochemistry, Vol. 91, Issue 1, p. 1-8
  • DOI: 10.1016/S0162-0134(02)00392-6

Crystallographic and FTIR Spectroscopic Evidence of Changes in Fe Coordination Upon Reduction of the Active Site of the Fe-Only Hydrogenase from Desulfovibrio d esulfuricans
journal, February 2001

  • Nicolet, Yvain; de Lacey, Antonio L.; Vernède, Xavier
  • Journal of the American Chemical Society, Vol. 123, Issue 8, p. 1596-1601
  • DOI: 10.1021/ja0020963

A novel FeS cluster in Fe-only hydrogenases
journal, March 2000

  • Nicolet, Yvain; Lemon, Brian J.; Fontecilla-Camps, Juan C.
  • Trends in Biochemical Sciences, Vol. 25, Issue 3, p. 138-143
  • DOI: 10.1016/S0968-0004(99)01536-4

Desulfovibrio desulfuricans iron hydrogenase: the structure shows unusual coordination to an active site Fe binuclear center
journal, January 1999


Carbamoylphosphate requirement for synthesis of the active center of [NiFe]-hydrogenases
journal, January 2001


Carbon Monoxide and Cyanide as Intrinsic Ligands to Iron in the Active Site of [NiFe]-Hydrogenases: NiFe(CN)
journal, February 1999

  • Pierik, Antonio J.; Roseboom, Winfried; Happe, Randolph P.
  • Journal of Biological Chemistry, Vol. 274, Issue 6
  • DOI: 10.1074/jbc.274.6.3331

Expression of a functional NAD-reducing [NiFe] hydrogenase from the gram-positive Rhodococcus opacus in the gram-negative Ralstonia eutropha
journal, November 2001

  • Porthun, Antje; Bernhard, Michael; Friedrich, Bärbel
  • Archives of Microbiology, Vol. 177, Issue 2, p. 159-166
  • DOI: 10.1007/s00203-001-0371-5

Discovery of Two Novel Radical S -Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase
journal, April 2004

  • Posewitz, Matthew C.; King, Paul W.; Smolinski, Sharon L.
  • Journal of Biological Chemistry, Vol. 279, Issue 24
  • DOI: 10.1074/jbc.M403206200

Structure-function relationships among the nickel-containing hydrogenases
journal, February 1992


Development of gfp vectors for expression in Listeria monocytogenes and other low G+C gram positive bacteria
journal, May 2001

  • Qazi, S. N. A.; Rees, C. E. D.; Mellits, K. H.
  • Microbial Ecology, Vol. 41, Issue 4
  • DOI: 10.1007/s002480000091

Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization
journal, June 1991

  • Rygus, T.; Scheler, A.; Allmansberger, R.
  • Archives of Microbiology, Vol. 155, Issue 6
  • DOI: 10.1007/BF00245346

A simple energy-conserving system: Proton reduction coupled to proton translocation
journal, June 2003

  • Sapra, R.; Bagramyan, K.; Adams, M. W. W.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 13, p. 7545-7550
  • DOI: 10.1073/pnas.1331436100

Applications of a peptide ligand for streptavidin: the Strep-tag
journal, December 1999


Protein production by auto-induction in high-density shaking cultures
journal, May 2005


BLAST 2 Sequences, a new tool for comparing protein and nucleotide sequences
journal, May 1999


De Novo Design of Synthetic Di-Iron(I) Complexes as Structural Models of the Reduced Form of Iron−Iron Hydrogenase
journal, February 2006

  • Tye, Jesse W.; Darensbourg, Marcetta Y.; Hall, Michael B.
  • Inorganic Chemistry, Vol. 45, Issue 4
  • DOI: 10.1021/ic051231f

Classification and phylogeny of hydrogenases
journal, August 2001


Occurrence, Classification, and Biological Function of Hydrogenases:  An Overview
journal, October 2007

  • Vignais, Paulette M.; Billoud, Bernard
  • Chemical Reviews, Vol. 107, Issue 10, p. 4206-4272
  • DOI: 10.1021/cr050196r

Structure of the [NiFe] Hydrogenase Active Site:  Evidence for Biologically Uncommon Fe Ligands
journal, January 1996

  • Volbeda, Anne; Garcin, Elsa; Piras, Claudine
  • Journal of the American Chemical Society, Vol. 118, Issue 51
  • DOI: 10.1021/ja962270g

The active site and catalytic mechanism of NiFe hydrogenases
journal, January 2003

  • Volbeda, Anne; Fontecilla-Camps, Juan C.
  • Dalton Trans., Issue 21
  • DOI: 10.1039/B304316A

In vitro hydrogen production by glucose dehydrogenase and hydrogenase
journal, July 1996

  • Woodward, Jonathan; Mattingly, Susan M.; Danson, Michael
  • Nature Biotechnology, Vol. 14, Issue 7, p. 872-874
  • DOI: 10.1038/nbt0796-872

High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway
journal, May 2007

  • Zhang, Y.-H. Percival; Evans, Barbara R.; Mielenz, Jonathan R.
  • PLoS ONE, Vol. 2, Issue 5, Article No. e456
  • DOI: 10.1371/journal.pone.0000456

Theoretical Studies of [FeFe]-Hydrogenase:  Infrared Fingerprints of the Dithiol-Bridging Ligand in the Active Site
journal, February 2007

  • Zilberman, Silviu; Stiefel, Edward I.; Cohen, Morrel H.
  • Inorganic Chemistry, Vol. 46, Issue 4
  • DOI: 10.1021/ic061432z

Effect of nickel on activity and subunit composition of purified hydrogenase from Nocardia opaca 1b
journal, February 1984


Conjugal transfer of hydrogen-oxidizing ability of Alcaligeneshydrogenophilus to Pseudomonasoxalaticus
journal, May 1986

  • Umeda, F.; Min, H.; Urushihara, M.
  • Biochemical and Biophysical Research Communications, Vol. 137, Issue 1, p. 108-113
  • DOI: 10.1016/0006-291X(86)91182-4

Isolation of hydrogen-oxidation gene from Alcaligeneshydrogenophilus and its expression in Pseudomonasoxalaticus
journal, May 1986

  • Yagi, Kiyohito; Min, Hongki; Urushihara, Masahiro
  • Biochemical and Biophysical Research Communications, Vol. 137, Issue 1, p. 114-119
  • DOI: 10.1016/0006-291X(86)91183-6