Consolidated bioprocessing method using thermophilic microorganisms

Mielenz, Jonathan Richard

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A - human necessities
A01 - agriculture
A21 - baking
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B - performing operations
B01 - physical or chemical processes or apparatus in general
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Title: Consolidated bioprocessing method using thermophilic microorganisms

Abstract

The present invention is directed to a method of converting biomass to biofuel, and particularly to a consolidated bioprocessing method using a co-culture of thermophilic and extremely thermophilic microorganisms which collectively can ferment the hexose and pentose sugars produced by degradation of cellulose and hemicelluloses at high substrate conversion rates. A culture medium therefor is also provided as well as use of the methods to produce and recover cellulosic ethanol.

Inventors:: Mielenz, Jonathan Richard

Issue Date:: 2016-02-02

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1237278

Patent Number(s):: 9249442

Application Number:: 13/762,619

Assignee:: UT-BATTELLE, LLC

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N1/20 - Bacteria {

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P2203/00 - Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
C12P39/00 - Processes involving microorganisms of different genera in the same process, simultaneously
C12P7/10 - substrate containing cellulosic material
C12P7/14 - Multiple stages of fermentation
C12P7/54 - Acetic acid

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
Y02E50/10 - Biofuels

Show less

DOE Contract Number:: AC05-00OR22725

Resource Type:: Patent

Resource Relation:: Patent File Date: 2013 Feb 08

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES

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                    Mielenz, Jonathan Richard. Consolidated bioprocessing method using thermophilic microorganisms.  United States: N. p., 2016. 
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                    Mielenz, Jonathan Richard. Consolidated bioprocessing method using thermophilic microorganisms.  United States.

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                    Mielenz, Jonathan Richard. Tue .  
        "Consolidated bioprocessing method using thermophilic microorganisms".  United States.  https://www.osti.gov/servlets/purl/1237278.

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@article{osti_1237278,

  title        = {Consolidated bioprocessing method using thermophilic microorganisms},

  author       = {Mielenz, Jonathan Richard},

  abstractNote = {The present invention is directed to a method of converting biomass to biofuel, and particularly to a consolidated bioprocessing method using a co-culture of thermophilic and extremely thermophilic microorganisms which collectively can ferment the hexose and pentose sugars produced by degradation of cellulose and hemicelluloses at high substrate conversion rates. A culture medium therefor is also provided as well as use of the methods to produce and recover cellulosic ethanol.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2016},

  month        = {2}

}

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Works referenced in this record:

Ethanol from Cellulosic Biomass [and Discussion]
journal, January 1983

Wang, D. I. C.; Avgerinos, G. C.; Biocic, I.
Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 300, Issue 1100
https://doi.org/10.1098/rstb.1983.0008

Thermophilic, lignocellulolytic bacteria for ethanol production: current state and perspectives
journal, July 2011

Chang, Tinghong; Yao, Shuo
Applied Microbiology and Biotechnology, Vol. 92, Issue 1
https://doi.org/10.1007/s00253-011-3456-3

Thermophilic ethanologenesis: future prospects for second-generation bioethanol production
journal, July 2009

Taylor, Mark P.; Eley, Kirsten L.; Martin, Steve
Trends in Biotechnology, Vol. 27, Issue 7, p. 398-405
https://doi.org/10.1016/j.tibtech.2009.03.006

Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield
journal, September 2008

Shaw, A. J.; Podkaminer, K. K.; Desai, S. G.
Proceedings of the National Academy of Sciences, Vol. 105, Issue 37, p. 13769-13774
https://doi.org/10.1073/pnas.0801266105

Molecular diversity of thermophilic cellulolytic and hemicellulolytic bacteria
journal, February 1999

Bergquist, P.
FEMS Microbiology Ecology, Vol. 28, Issue 2
https://doi.org/10.1016/S0168-6496(98)00078-6

Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002

Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
https://doi.org/10.1128/MMBR.66.3.506-577.2002

Polysaccharide Degradation and Synthesis by Extremely Thermophilic Anaerobes
journal, March 2008

VanFossen, Amy L.; Lewis, Derrick L.; Nichols, Jason D.
Annals of the New York Academy of Sciences, Vol. 1125, Issue 1, p. 322-337
https://doi.org/10.1196/annals.1419.017

Impact of Pretreated Switchgrass and Biomass Carbohydrates on Clostridium thermocellum ATCC 27405 Cellulosome Composition: A Quantitative Proteomic Analysis
journal, April 2009

Raman, Babu; Pan, Chongle; Hurst, Gregory B.
PLoS ONE, Vol. 4, Issue 4, Article No. e5271
https://doi.org/10.1371/journal.pone.0005271

Regulation of Cellulase Synthesis in Batch and Continuous Cultures of Clostridium thermocellum
journal, December 2004

Zhang, Y.-H. P.; Lynd, L. R.
Journal of Bacteriology, Vol. 187, Issue 1, p. 99-106
https://doi.org/10.1128/JB.187.1.99-106.2005

Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation
journal, January 2011

Raman, Babu; McKeown, Catherine K; Rodriguez, Miguel
BMC Microbiology, Vol. 11, Issue 1, Article No. 134
https://doi.org/10.1186/1471-2180-11-134

Extremely thermophilic microorganisms for biomass conversion status and prospects
journal, June 2008

Blumer-Schuette, Sara E.; Kataeva, Irina; Westpheling, Janet
Current Opinion in Biotechnology, Vol. 19, Issue 3, p. 210-217
https://doi.org/10.1016/j.copbio.2008.04.007

Complete Genome Sequences for the Anaerobic, Extremely Thermophilic Plant Biomass-Degrading Bacteria Caldicellulosiruptor hydrothermalis, Caldicellulosiruptor kristjanssonii, Caldicellulosiruptor kronotskyensis, Caldicellulosiruptor owensensis, and Caldicellulosiruptor lactoaceticus
journal, January 2011

Blumer-Schuette, S. E.; Ozdemir, I.; Mistry, D.
Journal of Bacteriology, Vol. 193, Issue 6, p. 1483-1484
https://doi.org/10.1128/JB.01515-10

Caldicellulosiruptor obsidiansis sp. nov., an Anaerobic, Extremely Thermophilic, Cellulolytic Bacterium Isolated from Obsidian Pool, Yellowstone National Park
journal, December 2009

Hamilton-Brehm, S. D.; Mosher, J. J.; Vishnivetskaya, T.
Applied and Environmental Microbiology, Vol. 76, Issue 4, p. 1014-1020
https://doi.org/10.1128/AEM.01903-09

Quantification of Cell and Cellulase Mass Concentrations during Anaerobic Cellulose Fermentation: Development of an Enzyme-Linked Immunosorbent Assay-Based Method with Application to Clostridium t hermocellum Batch Cultures
journal, January 2003

Zhang, Yiheng; Lynd, Lee R.
Analytical Chemistry, Vol. 75, Issue 2
https://doi.org/10.1021/ac020271n

A defined growth medium with very low background carbon for culturing Clostridium thermocellum
journal, February 2012

Holwerda, Evert K.; Hirst, Kyle D.; Lynd, Lee R.
Journal of Industrial Microbiology & Biotechnology, Vol. 39, Issue 6
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Characterization of 13 newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria
journal, November 2001

Ozkan, M.; Desai, Sg; Zhang, Y.
Journal of Industrial Microbiology and Biotechnology, Vol. 27, Issue 5
https://doi.org/10.1038/sj.jim.7000082

Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives
journal, March 2008

Kumar, Raj; Singh, Sompal; Singh, Om V.
Journal of Industrial Microbiology & Biotechnology, Vol. 35, Issue 5
https://doi.org/10.1007/s10295-008-0327-8

Multi-fuelling an engine
patent, December 2012

Courtoy, Pierre; Smith, Andrew; Greenhalgh, Douglas
US Patent Document 8,327,823
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8327823

Bioreactor for Mesophilic and/or Thermophilic Fermentation
patent-application, December 2010

Gray, Vincent Myles
US Patent Application 12/676643; 20100304420
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Similar Records

Title: Consolidated bioprocessing method using thermophilic microorganisms

Abstract

Citation Formats

Ethanol from Cellulosic Biomass [and Discussion] journal, January 1983

Thermophilic, lignocellulolytic bacteria for ethanol production: current state and perspectives journal, July 2011

Thermophilic ethanologenesis: future prospects for second-generation bioethanol production journal, July 2009

Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield journal, September 2008

Molecular diversity of thermophilic cellulolytic and hemicellulolytic bacteria journal, February 1999

Microbial Cellulose Utilization: Fundamentals and Biotechnology journal, September 2002

Polysaccharide Degradation and Synthesis by Extremely Thermophilic Anaerobes journal, March 2008

Impact of Pretreated Switchgrass and Biomass Carbohydrates on Clostridium thermocellum ATCC 27405 Cellulosome Composition: A Quantitative Proteomic Analysis journal, April 2009

Regulation of Cellulase Synthesis in Batch and Continuous Cultures of Clostridium thermocellum journal, December 2004

Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation journal, January 2011

Extremely thermophilic microorganisms for biomass conversion status and prospects journal, June 2008

Caldicellulosiruptor obsidiansis sp. nov., an Anaerobic, Extremely Thermophilic, Cellulolytic Bacterium Isolated from Obsidian Pool, Yellowstone National Park journal, December 2009

Quantification of Cell and Cellulase Mass Concentrations during Anaerobic Cellulose Fermentation: Development of an Enzyme-Linked Immunosorbent Assay-Based Method with Application to Clostridium t hermocellum Batch Cultures journal, January 2003

A defined growth medium with very low background carbon for culturing Clostridium thermocellum journal, February 2012

Characterization of 13 newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria journal, November 2001

Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives journal, March 2008

Multi-fuelling an engine patent, December 2012

Bioreactor for Mesophilic and/or Thermophilic Fermentation patent-application, December 2010

Ethanol from Cellulosic Biomass [and Discussion]
journal, January 1983

Thermophilic, lignocellulolytic bacteria for ethanol production: current state and perspectives
journal, July 2011

Thermophilic ethanologenesis: future prospects for second-generation bioethanol production
journal, July 2009

Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield
journal, September 2008

Molecular diversity of thermophilic cellulolytic and hemicellulolytic bacteria
journal, February 1999

Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002

Polysaccharide Degradation and Synthesis by Extremely Thermophilic Anaerobes
journal, March 2008

Impact of Pretreated Switchgrass and Biomass Carbohydrates on Clostridium thermocellum ATCC 27405 Cellulosome Composition: A Quantitative Proteomic Analysis
journal, April 2009

Regulation of Cellulase Synthesis in Batch and Continuous Cultures of Clostridium thermocellum
journal, December 2004

Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation
journal, January 2011

Extremely thermophilic microorganisms for biomass conversion status and prospects
journal, June 2008

Caldicellulosiruptor obsidiansis sp. nov., an Anaerobic, Extremely Thermophilic, Cellulolytic Bacterium Isolated from Obsidian Pool, Yellowstone National Park
journal, December 2009

Quantification of Cell and Cellulase Mass Concentrations during Anaerobic Cellulose Fermentation: Development of an Enzyme-Linked Immunosorbent Assay-Based Method with Application to Clostridium t hermocellum Batch Cultures
journal, January 2003

A defined growth medium with very low background carbon for culturing Clostridium thermocellum
journal, February 2012

Characterization of 13 newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria
journal, November 2001

Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives
journal, March 2008

Multi-fuelling an engine
patent, December 2012

Bioreactor for Mesophilic and/or Thermophilic Fermentation
patent-application, December 2010