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Title: Compositions and methods for pathogen transport

This disclosure provides a method for transporting a pathogen under ambient conditions, by culturing the pathogen with an amoeba under conditions that favor the incorporation of the pathogen into a trophozoite, starving the amoeba until it encysts, then culturing under conditions that favor conversion of the amoeba back to a trophozoite. In one aspect, the conditions that favor incorporation of the pathogen into the cyst of the amoeba comprises contacting the pathogen with the amoeba in an iron rich environment. Virus and/or bacteria are pathogens that can be transported by the disclosed method. Amoeba that are useful in the disclosed methods include, without limitation Acanthamoeba castellanii, Hartmannella vermiformis and Naegleria gruberi. The disclosed methods have utility in: transporting pathogens from military field hospitals and clinics to the laboratory; transporting pathogens from global satellite laboratories to clinical laboratories; long term storage of pathogens; enriching contaminated patient samples for pathogens of interest; biosurveillance and detection efforts.
Inventors:
;
Issue Date:
OSTI Identifier:
1236565
Assignee:
Lawrence Livermore National Security, LLC LLNL
Patent Number(s):
9,243,222
Application Number:
14/148,484
Contract Number:
AC52-07NA27344
Resource Relation:
Patent File Date: 2014 Jan 06
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Works referenced in this record:

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Acanthamoeba castellanii: Growth, encystment, excystment and biocide susceptibility
journal, January 1998

The Neff strain of Acanthamoeba castellanii, a tool for testing the virulence of Mycobacterium kansasii
journal, May 2007
  • Goy, Geneviève; Thomas, Vincent; Rimann, Katia
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