DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Keto-isovalerate decarboxylase enzymes and methods of use thereof

Abstract

Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
Butamax Advanced Biofuels LLC, Wilmington, DE (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1236558
Patent Number(s):
9238828
Application Number:
13/560,147
Assignee:
Butamax Advanced Biofuels LLC
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AR0000006
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Jul 27
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 60 APPLIED LIFE SCIENCES

Citation Formats

McElvain, Jessica, O'Keefe, Daniel P., Paul, Brian James, Payne, Mark S., Rothman, Steven Cary, and He, Hongxian. Keto-isovalerate decarboxylase enzymes and methods of use thereof. United States: N. p., 2016. Web.
McElvain, Jessica, O'Keefe, Daniel P., Paul, Brian James, Payne, Mark S., Rothman, Steven Cary, & He, Hongxian. Keto-isovalerate decarboxylase enzymes and methods of use thereof. United States.
McElvain, Jessica, O'Keefe, Daniel P., Paul, Brian James, Payne, Mark S., Rothman, Steven Cary, and He, Hongxian. Tue . "Keto-isovalerate decarboxylase enzymes and methods of use thereof". United States. https://www.osti.gov/servlets/purl/1236558.
@article{osti_1236558,
title = {Keto-isovalerate decarboxylase enzymes and methods of use thereof},
author = {McElvain, Jessica and O'Keefe, Daniel P. and Paul, Brian James and Payne, Mark S. and Rothman, Steven Cary and He, Hongxian},
abstractNote = {Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {1}
}

Works referenced in this record:

Use of the valine biosynthetic pathway to convert glucose into isobutanol
journal, December 2010


Branched-Chain Keto Acid Decarboxylase from Lactococcus lactis (KdcA), a Valuable Thiamine Diphosphate-Dependent Enzyme for Asymmetric C-C Bond Formation
journal, June 2007


Application of α-keto acid decarboxylases in biotransformations
journal, June 1998


Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes
journal, July 2009


Aspartate-27 and glutamate-473 are involved in catalysis by Zymomonas mobilis pyruvate decarboxylase
journal, April 1999


The role of induced fit and conformational changes of enzymes in specificity and catalysis
journal, March 1988


Identification, Cloning, and Characterization of a Lactococcus lactis Branched-Chain  -Keto Acid Decarboxylase Involved in Flavor Formation
journal, January 2005


Determinants of substrate specificity in KdcA, a thiamin diphosphate-dependent decarboxylase
journal, December 2006


Solvent tolerant microorganisms and methods of isolation
patent, June 2009


Fermentive production of four carbon alcohols
patent, December 2010


Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
patent, March 2011


Fermentive production of four carbon alcohols
patent, August 2011


Solvent tolerant microorganisms
patent, September 2011


Ketol-acid reductoisomerase using NADH
patent, March 2012


Fermentive production of four carbon alcohols
patent, May 2012


Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
patent, May 2012


Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase
patent, June 2012


Ketol-acid reductoisomerase using NADH
patent, July 2012


Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof
patent, August 2012


Fermentive production of four carbon alcohols
patent, September 2012


Fermentive production of four carbon alcohols
patent, October 2012


Production of four carbon alcohols using improved strain
patent, February 2013


Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway
patent, June 2013


Strain comprising increased expression of a CFA coding region for butanol production
patent, August 2013


Yeast with increased butanol tolerance involving filamentous growth response
patent, October 2013


Expression of hexose kinase in recombinant host cells
patent, January 2014


Enhanced pyruvate to acetolactate conversion in yeast
patent, March 2014


Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
patent, April 2014


Fermentive production of four carbon alcohols
patent, May 2014


Alcohol dehydrogenases (ADH) useful for fermentive production of lower alkyl alcohols
patent, July 2014


Yeast with increased butanol tolerance involving cell wall integrity pathway
patent, August 2014


Production of isobutanol in yeast mitochondria
patent, September 2014


Fermentive production of four carbon alcohols
patent, September 2014


Fermentive Production Of Four Carbon Alcohols
patent-application, July 2008


Identification and use of Bacterial [2FE-2S] Dihydroxy-acid Dehydratases
patent-application, April 2010


Carbon Pathway Optimized Production Hosts for the Production of Isobutanol
patent-application, May 2010


Cytosolic Isobutanol Pathway Localization for the Production of Isobutanol
patent-application, March 2011


Bacterial Strains for Butanol Production
patent-application, August 2011


Fermentive Production of Isobutanol Using Highly Effective Ketol-acid Reductoisomerase Enzymes
patent-application, October 2011


Engineering Resistance to Aliphatic Alcohols
patent-application, March 2012


Recombinant host cells comprising phosphoketolases
patent-application, June 2012


Fermentive Production of Four Carbon Alcohols
patent-application, August 2012


Lignocellulosic Hydrolysates as Feedstocks for Isobutanol Fermentation
patent-application, February 2013


Acetate Supplementation of Medium for Butanologens
patent-application, September 2013


Host Cells and Methods for Production of Isobutanol
patent-application, January 2014


Host Cells and Methods for Production of Isobutanol
patent-application, January 2014


Activity of FE-S Cluster Requiring Proteins
patent-application, February 2014


Dhad Variants for Butanol Production
patent-application, July 2014