Keto-isovalerate decarboxylase enzymes and methods of use thereof

Title: Keto-isovalerate decarboxylase enzymes and methods of use thereof

Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.

Inventors:: McElvain, Jessica; O'Keefe, Daniel P.; Paul, Brian James; Payne, Mark S.; Rothman, Steven Cary; He, Hongxian

Issue Date:: 2016-01-19

OSTI Identifier:: 1236558

Assignee:: Butamax Advanced Biofuels LLC ARPA-E

Patent Number(s):: 9,238,828

Application Number:: 13/560,147

Contract Number:: AR0000006

Resource Relation:: Patent File Date: 2012 Jul 27

Research Org:: Butamax Advanced Biofuels LLC, Wilmington, DE (United States)

Sponsoring Org:: USDOE

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 60 APPLIED LIFE SCIENCES

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Works referenced in this record:

Branched-Chain Keto Acid Decarboxylase from Lactococcus lactis (KdcA), a Valuable Thiamine Diphosphate-Dependent Enzyme for Asymmetric C-C Bond Formation
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Gocke, Dörte; Nguyen, Cong Luan; Pohl, Martina
Advanced Synthesis & Catalysis, Vol. 349, Issue 8-9, p. 1425-1435
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Structure of the branched-chain keto acid decarboxylase (KdcA) from Lactococcus lactis provides insights into the structural basis for the chemoselective and enantioselective carboligation reaction
journal, November 2007

Berthold, Catrine L.; Gocke, Dörte; Wood, Martin D.
Acta Crystallographica Section D Biological Crystallography, Vol. 63, Issue 12, p. 1217-1224
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The role of induced fit and conformational changes of enzymes in specificity and catalysis
journal, March 1988

Herschlag, Daniel
Bioorganic Chemistry, Vol. 16, Issue 1, p. 62-96
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Determinants of substrate specificity in KdcA, a thiamin diphosphate-dependent decarboxylase
journal, December 2006

Yep, Alejandra; Kenyon, George L.; McLeish, Michael J.
Bioorganic Chemistry, Vol. 34, Issue 6, p. 325-336
DOI: 10.1016/j.bioorg.2006.08.005

Alcohol dehydrogenases (ADH) useful for fermentive production of lower alkyl alcohols
patent, July 2014

Satagopan, Sriram; O'Keefe, Daniel; Gude, Janardhan
US Patent Document 8,765,433
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=/netahtml/PTO/search-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN/8765433

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