Methods for point-of-care detection of nucleic acid in a sample

Bearinger, Jane P.; Dugan, Lawrence C.

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Title: Methods for point-of-care detection of nucleic acid in a sample

Abstract

Provided herein are methods and apparatus for detecting a target nucleic acid in a sample and related methods and apparatus for diagnosing a condition in an individual. The condition is associated with presence of nucleic acid produced by certain pathogens in the individual.

Inventors:: Bearinger, Jane P.; Dugan, Lawrence C.

Issue Date:: 2015-12-29

Research Org.:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1233498

Patent Number(s):: 9222126

Application Number:: 13/115,878

Assignee:: Lawrence Livermore National Security, LLC

Patent Classifications (CPCs):: B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2300/0609 - Holders integrated in container to position an object
B01L2300/0851 - Bottom walls
B01L2300/1877 - using chemical reactions
B01L3/502776 - {specially adapted for focusing or laminating flows}
B01L3/5029 - {using swabs}
B01L7/52 - {with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/6844 - Nucleic acid amplification reactions
C12Q1/689 - for bacteria
C12Q1/701 - {Specific hybridization probes}
C12Q2537/101 - Homogeneous assay format, e.g. one pot reaction

Show less

DOE Contract Number:: AC52-07NA27344

Resource Type:: Patent

Resource Relation:: Patent File Date: 2011 May 25

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats


                    Bearinger, Jane P., and Dugan, Lawrence C. Methods for point-of-care detection of nucleic acid in a sample.  United States: N. p., 2015. 
        Web.

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                    Bearinger, Jane P., & Dugan, Lawrence C. Methods for point-of-care detection of nucleic acid in a sample.  United States.

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                    Bearinger, Jane P., and Dugan, Lawrence C. Tue .  
        "Methods for point-of-care detection of nucleic acid in a sample".  United States.  https://www.osti.gov/servlets/purl/1233498.

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@article{osti_1233498,

  title        = {Methods for point-of-care detection of nucleic acid in a sample},

  author       = {Bearinger, Jane P. and Dugan, Lawrence C.},

  abstractNote = {Provided herein are methods and apparatus for detecting a target nucleic acid in a sample and related methods and apparatus for diagnosing a condition in an individual. The condition is associated with presence of nucleic acid produced by certain pathogens in the individual.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2015},

  month        = {12}

}

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Similar Records

Title: Methods for point-of-care detection of nucleic acid in a sample

Abstract

Citation Formats

Assessment of DNA extracted from FTA® cards for use on the Illumina iSelect BeadChip journal, January 2009

Buccal Swabs and Treated Cards: Methodological Considerations for Molecular Epidemiologic Studies Examining Pediatric Populations journal, March 2008

Performance of Real-Time Reverse Transcription Polymerase Chain Reaction for the Detection of Foot-and-Mouth Disease Virus during Field Outbreaks in the United Kingdom in 2007 journal, May 2009

Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue journal, March 2009

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Helicase-dependent isothermal DNA amplification journal, August 2004

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Novel reverse transcription loop-mediated isothermal amplification for rapid detection of foot-and-mouth disease virus journal, February 2006

Comparisons of original laboratory results and retrospective analysis by real-time reverse transcriptase-PCR of virological samples collected from confirmed cases of foot-and-mouth disease in the UK in 2001 journal, September 2006

Development and Initial Results of a Low Cost, Disposable, Point-of-Care Testing Device for Pathogen Detection journal, October 2010

Rapid, Point-of-Care Extraction of Human Immunodeficiency Virus Type 1 Proviral DNA from Whole Blood for Detection by Real-Time PCR journal, July 2009

A polymer lab-on-a-chip for reverse transcription (RT)-PCR based point-of-care clinical diagnostics journal, January 2008

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System for the temperature adjustment treatment of liquid samples patent, July 1999

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Syringe devices and methods for mixing and administering medication patent, August 2010

Integrated PCR Reactor for Cell Lysis, Nucleic Acid Isolation and Purification, and Nucleic Acid Amplification Related Applications patent-application, July 2009

Assessment of DNA extracted from FTA® cards for use on the Illumina iSelect BeadChip
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Buccal Swabs and Treated Cards: Methodological Considerations for Molecular Epidemiologic Studies Examining Pediatric Populations
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Performance of Real-Time Reverse Transcription Polymerase Chain Reaction for the Detection of Foot-and-Mouth Disease Virus during Field Outbreaks in the United Kingdom in 2007
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Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue
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Loop-mediated isothermal amplification of DNA
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DNA Amplification and Detection Made Simple (Relatively)
journal, June 2006

Novel reverse transcription loop-mediated isothermal amplification for rapid detection of foot-and-mouth disease virus
journal, February 2006

Comparisons of original laboratory results and retrospective analysis by real-time reverse transcriptase-PCR of virological samples collected from confirmed cases of foot-and-mouth disease in the UK in 2001
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Development and Initial Results of a Low Cost, Disposable, Point-of-Care Testing Device for Pathogen Detection
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Rapid, Point-of-Care Extraction of Human Immunodeficiency Virus Type 1 Proviral DNA from Whole Blood for Detection by Real-Time PCR
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A polymer lab-on-a-chip for reverse transcription (RT)-PCR based point-of-care clinical diagnostics
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Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems
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System for the temperature adjustment treatment of liquid samples
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